FastQCFastQC Report
Mon 6 Jun 2016
ERR522928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4740133
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181570.38304832374956566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143560.30286070032212176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93500.1972518492624574No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50110.10571433333199723No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87850.047.2434271
TACCTGG47750.041.637982
GTATCAA153150.039.5435941
GTACCTG58850.034.4623871
ACCTGGG59950.032.218243
TCAACGC187000.031.8398064
ATCAACG188100.031.6546123
CAACGCA190400.031.27195
TATCAAC192400.031.12342
AACGCAG197800.030.4509376
TATCACG4300.027.3271452
ACGCAGA222700.026.982947
GTACATG149050.026.1404421
CGCAGAG229550.026.1348768
TACATGG153900.024.6160932
GCAGAGT252300.023.460999
ACATGGG156350.023.3545933
CCTGGGG61200.022.4216144
ATAACGC4500.021.9308743
TATAACG4550.021.6935482
Produced by FastQC (version 0.11.5)