FastQCFastQC Report
Mon 6 Jun 2016
ERR522928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4740133
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162710.343260410625609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140030.2954136518954215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88840.18742090148103438No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90700.044.1427271
TACCTGG51750.041.627662
GTATCAA151000.036.952571
GTACCTG63850.034.4843331
ACCTGGG63700.033.12623
TATCACG5900.029.4969142
TCAACGC186750.029.4677734
ATCAACG189100.029.0776353
CAACGCA190700.028.85745
TATCAAC193850.028.5586382
AACGCAG197750.028.1610156
GTACATG143050.025.8177661
ATAACGC5350.025.4746953
TATAACG5000.025.3992312
TAACGCA5600.025.1758564
TACATGG145100.024.798242
ACGCAGA225550.024.7311977
CGCAGAG231550.024.0939128
ACATGGG150650.023.0848663
CCTGGGG61150.022.3639014
Produced by FastQC (version 0.11.5)