FastQCFastQC Report
Mon 6 Jun 2016
ERR522927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8467060
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT904371.0681039227311486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267670.316130982891346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210400.248492392873087No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC202110.23870150914248864No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC186050.21973388637850683No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA150540.17779488984370018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119330.1409343975358625No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC114760.13553701048533967No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG294250.025.9160671
TACATGG299850.024.8973352
ACATGGG304150.023.8506373
CATGGGG196600.021.424994
GAGTACT234850.020.76533912-13
GTACTTT252700.019.05689414-15
AGAGTAC370050.019.00449410-11
AGTACTT249450.017.64770912-13
GAGTACA235650.017.4971351
ATGGGGG112250.017.3810445
AGTACAT226600.016.8462222
GTATAGC27750.016.6034321
CATGGGA115550.016.3550624
ACTTTTT307850.015.74869116-17
TACTTTT303900.014.43941914-15
GTGTAGC47550.014.4356751
TATACTG34450.014.0560125
GTATAGG24450.013.6525651
GTATAAT36750.013.5607371
TAGCCCT52400.013.4572434
Produced by FastQC (version 0.11.5)