FastQCFastQC Report
Mon 6 Jun 2016
ERR522927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8467060
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT902241.065588291567557No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT258300.3050645678665322No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237640.2806641266271882No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC207530.24510278656345885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199920.2361150151292184No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC193660.22872165781274728No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG152580.18020422673277384No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA151960.17947197728609457No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA129630.1530991867306952No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC117290.13852506064678885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113480.13402526969219541No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG292900.026.6990531
TACATGG298000.025.614292
ACATGGG302300.024.3914953
CATGGGG193650.022.4481434
GAGTACT246600.020.06523512-13
ATGGGGG107300.018.7893265
GTACTTT262500.018.6140614-15
AGAGTAC379150.018.40069410-11
AGTACTT258650.017.84901412-13
GAGTACA230100.017.6980021
GTATAGG24100.017.5609661
AGTACAT221300.016.8211772
CATGGGA113850.016.2224224
ACTTTTT320700.015.37258116-17
GTGTAGC48750.015.14425851
GTATAAT37400.015.0880331
TACTTTT304700.015.07190214-15
GTATAGC27800.014.3779551
GTATAAC25550.013.9876791
TATACTG36700.013.95770555
Produced by FastQC (version 0.11.5)