FastQCFastQC Report
Mon 6 Jun 2016
ERR522926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8225863
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200590.2438528334352274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157240.19115319571940353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88830.10798866939553942No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118450.046.162071
GTATCAA210500.039.835461
TCAACGC246700.033.09024
ATCAACG252150.032.4146273
CAACGCA251550.032.413655
AACGCAG261250.031.29196
TATCAAC263900.031.0826022
ACGCAGA301450.027.1343787
CGCAGAG313950.026.038268
GTACATG254200.025.8048551
TACATGG256500.024.7404582
TACCTGG70950.024.712562
ACATGGG260650.023.6939833
GCAGAGT351050.023.0306349
CATGGGG191200.021.9253064
GAGTACT202400.020.78647612-13
GTACCTG89150.020.5325321
AGAGTAC311050.019.87545610-11
CAGAGTA340250.019.5297310-11
ATGGGGG113750.018.9219915
Produced by FastQC (version 0.11.5)