FastQCFastQC Report
Mon 6 Jun 2016
ERR522926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8225863
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177380.21563694897422922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148400.18040660293029437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117750.046.506651
GTATCAA202400.038.7943761
TCAACGC239550.032.28984
ATCAACG244000.031.7213233
CAACGCA244550.031.5719645
AACGCAG253800.030.4762826
TATCAAC255200.030.4653952
ACGCAGA292800.026.3846827
TACCTGG69800.025.6734492
GTACATG250550.025.6307451
CGCAGAG306850.025.1669318
TACATGG256650.024.4472052
ACATGGG263250.023.1536333
GCAGAGT347500.022.0063959
CATGGGG188300.021.362654
GAGTACT192400.020.27681512-13
GTACCTG91300.020.0448041
AGAGTAC306450.019.27200710-11
CAGAGTA334950.019.17581210-11
ATGGGGG110050.019.1302075
Produced by FastQC (version 0.11.5)