FastQCFastQC Report
Mon 6 Jun 2016
ERR522925_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522925_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9599766
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1177141.226217389048858No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT324200.33771656517460946No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC255070.26570439321125117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253210.2637668459835375No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC243170.2533082577221153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192710.20074447647994753No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG192670.20070280879763114No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA183540.1911921603089075No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA153050.159430969463214No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC150930.15722258230044356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120930.12597182056312622No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG102810.10709636047378655No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG382100.030.701451
TACATGG384350.029.9933432
ACATGGG400850.028.07983
CATGGGG246700.025.7840334
ATGGGGG143300.022.3105325
CATGGGA150100.019.9517484
GAGTACA279100.019.6259861
GAGTACT234950.019.22597112-13
AGTACAT271400.019.124522
AGAGTAC390650.018.83729210-11
GTACTTT251150.017.71492214-15
AGTACTT250300.017.16474512-13
GTATAGC30100.016.8702931
GTGTAGC53200.016.792191
GTATAGG25350.016.692831
TATACTG39950.015.4170655
ATGGGAG76000.015.3421185
ATGGGGA102150.015.1887765
TACCTGG62200.014.663672
CATGGGT66900.014.617044
Produced by FastQC (version 0.11.5)