FastQCFastQC Report
Mon 6 Jun 2016
ERR522925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9599766
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1207451.2577910753241277No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT331100.3449042403741925No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC264860.2759025584581958No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC248120.2584646334087727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237470.24737061299202504No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA193440.20150491168222226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189390.1972860588476844No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA161520.1682541011937166No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC157870.16445192518234297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114490.11926332371018211No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG105250.10963808909508838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG384350.029.1711221
TACATGG388300.028.3503132
ACATGGG397750.026.9041233
CATGGGG244550.024.0603894
ATGGGGG140550.021.0652525
GAGTACT243250.019.61783812-13
CATGGGA153150.019.2711944
AGAGTAC412050.019.04329510-11
GAGTACA290250.018.925371
GTACTTT256200.018.4118814-15
AGTACAT277650.018.4022222
AGTACTT255900.018.13363312-13
GTATACG5250.016.1245251
GTATAGG28900.015.9478441
GTATAGA35450.015.6545111
TAGCCCT54700.015.6368794
CATGGGT63650.015.505544
ATGGGAG74300.015.3067685
TACTTTT302650.015.11235114-15
GTGTAGC55550.015.0698771
Produced by FastQC (version 0.11.5)