FastQCFastQC Report
Mon 6 Jun 2016
ERR522924_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522924_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12301210
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA142580.1159072969244489No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG201000.050.7199942
GTACCTG249800.041.2542531
ACCTGGG246200.040.625193
CCTGGGG240700.029.6331374
TGGGGGG181700.025.4748736
CATGGGG370950.025.2235284
GTACATG624050.024.6535741
ATGGGGG215900.024.3464725
TACATGG640900.023.8383062
ACATGGG652400.022.7045333
CTGGGGG144350.021.561955
TATACCG10550.019.608625
TATCACG9900.019.4744242
GTATCAA395450.018.7568041
GTACACG29600.017.0024241
CAACGCA426350.016.9494045
TCAACGC426300.016.9301624
AACGCAG444700.016.4110016
ATCAACG443200.016.275773
TATCAAC447000.016.2320882
Produced by FastQC (version 0.11.5)