##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522924_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12301210
Sequences flagged as poor quality	0
Sequence length	100
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28789362997624	33.0	31.0	34.0	28.0	34.0
2	31.64039017299924	34.0	31.0	34.0	27.0	35.0
3	31.62560431047027	34.0	31.0	34.0	27.0	36.0
4	33.9147487117121	35.0	33.0	37.0	30.0	37.0
5	33.98918285274375	35.0	34.0	37.0	30.0	37.0
6	34.20806245889632	35.0	35.0	37.0	30.0	37.0
7	34.07718614672866	35.0	34.0	37.0	30.0	37.0
8	34.16442317463079	35.0	34.0	37.0	30.0	37.0
9	35.34341833039189	37.0	34.0	39.0	30.0	39.0
10-11	35.30046271870816	37.0	34.5	39.0	30.0	39.0
12-13	35.31803639641954	37.0	35.0	39.0	30.0	39.0
14-15	36.10460974977258	38.0	35.0	40.0	30.0	41.0
16-17	36.248310288174906	38.0	36.0	40.0	30.0	41.0
18-19	36.0603422346257	38.0	35.0	40.0	29.5	41.0
20-21	36.138049143133074	38.0	35.0	40.0	29.0	41.0
22-23	35.998358657400374	38.0	35.0	40.0	29.0	41.0
24-25	35.97088290501503	38.0	35.0	40.0	28.5	41.0
26-27	35.86258258333936	37.0	35.5	40.0	28.5	41.0
28-29	35.623629138922105	37.5	34.5	40.0	28.0	41.0
30-31	35.473975893428374	37.0	35.0	40.0	27.0	41.0
32-33	35.491272362637496	37.5	34.5	40.0	27.0	41.0
34-35	35.37548420033477	37.5	34.5	40.0	27.0	41.0
36-37	35.22805232168218	37.5	34.5	40.0	27.0	41.0
38-39	35.08676231850363	36.5	34.5	40.0	26.5	41.0
40-41	35.05273058504001	37.0	33.5	40.0	26.0	41.0
42-43	34.87226033048781	36.0	34.0	40.0	26.0	41.0
44-45	34.82539770477864	37.0	33.5	40.0	26.0	41.0
46-47	34.68367416701284	36.5	33.5	40.0	25.0	41.0
48-49	34.60584003524856	37.0	33.0	40.0	24.5	41.0
50-51	33.58780022453076	36.5	32.0	38.5	24.0	40.0
52-53	33.81246462746348	36.5	32.5	38.5	25.0	39.5
54-55	34.695158768934114	37.0	33.0	39.5	26.0	40.5
56-57	34.8411757867722	37.0	34.0	40.0	26.0	41.0
58-59	34.7463675931067	37.0	34.0	40.0	26.0	41.0
60-61	34.47687560817188	36.5	34.0	39.0	26.0	41.0
62-63	34.27239377264513	36.0	33.0	39.0	26.0	41.0
64-65	33.864254126220104	36.0	33.0	39.0	25.0	40.5
66-67	33.66181534174281	36.0	33.0	38.0	25.5	40.0
68-69	33.316895248516204	35.0	32.5	37.5	24.0	40.0
70-71	33.099636133355986	35.0	32.0	37.0	24.0	39.0
72-73	32.77224273059317	35.0	32.0	37.0	23.5	39.0
74-75	32.39446367471167	35.0	32.0	36.0	23.0	38.5
76-77	32.280444444083145	35.0	31.0	36.5	22.5	38.0
78-79	32.01820268087448	35.0	31.0	36.0	22.0	37.0
80-81	31.76894175451033	35.0	31.0	36.0	21.0	37.5
82-83	31.477270203500304	34.0	31.0	35.5	20.5	37.0
84-85	31.258552491990628	34.5	30.5	35.0	20.0	36.5
86-87	31.025909158529934	34.0	30.0	35.0	20.0	37.0
88-89	30.87948693665095	34.0	30.0	35.0	19.0	36.5
90-91	30.677122453807392	34.0	29.5	35.0	18.0	36.0
92-93	30.42429110632206	34.0	29.0	35.0	18.0	36.0
94-95	30.332804374529008	34.0	29.0	35.0	18.0	36.0
96-97	30.139797629664073	34.0	29.0	35.0	17.0	35.5
98-99	30.045362285498744	34.0	29.0	35.0	17.5	36.0
100	28.701493755492347	33.0	25.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22323425928680507
1101	2	0.2161737555558112
1101	3	0.21132335615009978
1101	4	0.18363868480456347
1101	5	0.220704667851777
1101	6	0.3051289680219682
1101	7	0.2735905693595839
1101	8	0.36458252050224615
1101	9	0.32235945857438963
1101	10-11	0.2751041969224417
1101	12-13	0.2392935551145854
1101	14-15	0.17128713040735022
1101	16-17	0.21704642284372966
1101	18-19	0.21912931053454798
1101	20-21	0.24513522207493565
1101	22-23	0.18146231800450607
1101	24-25	0.09080594288621313
1101	26-27	0.17654642262947107
1101	28-29	0.11161776326100181
1101	30-31	0.09481063770565612
1101	32-33	0.08099461149943465
1101	34-35	0.02968958949565348
1101	36-37	0.05585122098810302
1101	38-39	0.06870384525632289
1101	40-41	0.0500741348859961
1101	42-43	0.023505250863259164
1101	44-45	0.009015834694530156
1101	46-47	0.06541255878310182
1101	48-49	0.020525064684740357
1101	50-51	-0.14831563595058128
1101	52-53	0.0335904522272088
1101	54-55	0.09749407993397341
1101	56-57	0.05228628399594726
1101	58-59	0.06737160865951353
1101	60-61	0.018256324887133246
1101	62-63	-0.07222700928584658
1101	64-65	-0.03347683566502724
1101	66-67	-0.10366751539125119
1101	68-69	-0.06397972326327306
1101	70-71	-0.19421685830481294
1101	72-73	-0.10106303854202991
1101	74-75	-0.13043961353726274
1101	76-77	-0.07999262800638007
1101	78-79	-0.3227268562060637
1101	80-81	-0.2262897729012785
1101	82-83	-0.4278994281551256
1101	84-85	-0.3802585477371281
1101	86-87	-0.257110310222437
1101	88-89	-0.5025711818757266
1101	90-91	-0.49909761931833074
1101	92-93	-0.39823691310460774
1101	94-95	-0.47817598007185325
1101	96-97	-0.41129526387920023
1101	98-99	-0.37447475163880384
1101	100	-0.517695200145333
1102	1	0.04943175679302669
1102	2	-4.583150806389824E-4
1102	3	0.03795940020330946
1102	4	-0.02189551959871494
1102	5	0.024296125528152857
1102	6	0.07257827104738368
1102	7	0.03297907508738973
1102	8	0.14456134223357964
1102	9	0.03535030749144852
1102	10-11	0.054392197172354884
1102	12-13	0.027224305027921503
1102	14-15	-0.07607888562914411
1102	16-17	0.017144099787657296
1102	18-19	0.08638565070154414
1102	20-21	-0.00909355543913648
1102	22-23	-0.033460628152447214
1102	24-25	-0.07816095260152878
1102	26-27	-0.051314272569292996
1102	28-29	-0.010143312364597534
1102	30-31	-0.12873352905915425
1102	32-33	-0.1459834483683764
1102	34-35	-0.20791472895384544
1102	36-37	-0.5873991739286168
1102	38-39	-0.282692742164933
1102	40-41	-0.9766673123741114
1102	42-43	-0.9971470053205707
1102	44-45	-0.42331978432260087
1102	46-47	-0.3710712025709597
1102	48-49	-0.4012843287572494
1102	50-51	-0.478209291507504
1102	52-53	-0.3243761127054299
1102	54-55	-0.21696002584364749
1102	56-57	-0.12052349720681832
1102	58-59	-0.11780773564636604
1102	60-61	-0.18129027047543644
1102	62-63	-0.4461926906215652
1102	64-65	-0.2019430276313443
1102	66-67	-0.2017175724979765
1102	68-69	-0.15765573155859158
1102	70-71	-0.240725772463243
1102	72-73	-0.16176538382021022
1102	74-75	-0.24169788085020372
1102	76-77	-0.3741059443248247
1102	78-79	-0.33513226726720546
1102	80-81	-0.438345990863521
1102	82-83	-0.5113414983130227
1102	84-85	-0.6282571725350508
1102	86-87	-0.5302339407872552
1102	88-89	-0.5180471006182934
1102	90-91	-0.49549390647971947
1102	92-93	-0.6026986404546228
1102	94-95	-0.7123027563684161
1102	96-97	-0.8748695180323978
1102	98-99	-0.8318518372533852
1102	100	-0.7918474803828204
1103	1	0.0077615825180856746
1103	2	-0.06616633850771336
1103	3	-4.585427663421626E-4
1103	4	0.024105090371769222
1103	5	0.053820592502198394
1103	6	0.08996188577805952
1103	7	0.09589433798048219
1103	8	-0.0885408809389574
1103	9	0.041455764937936124
1103	10-11	0.02967413114006945
1103	12-13	0.05970547729858566
1103	14-15	0.04216676470574754
1103	16-17	-5.022958148117596E-4
1103	18-19	0.0036302623792039412
1103	20-21	-0.13736487129003905
1103	22-23	-0.06517259182265178
1103	24-25	-0.11970147493278915
1103	26-27	-0.12131633642809447
1103	28-29	-0.29631738838143207
1103	30-31	-0.2362619614632635
1103	32-33	-0.3086932419762789
1103	34-35	-0.3829368954362806
1103	36-37	-0.4631815978967211
1103	38-39	-0.43399799505166214
1103	40-41	-0.3528493952076488
1103	42-43	-0.29179657362892186
1103	44-45	-0.29304103292636086
1103	46-47	-0.18725371776609023
1103	48-49	-0.3415537362525285
1103	50-51	-0.43370285199100067
1103	52-53	-0.1945011755950361
1103	54-55	-0.1406793655546963
1103	56-57	0.005185563803287607
1103	58-59	-0.07966544969815459
1103	60-61	-0.07197330767051824
1103	62-63	-0.06437740892449284
1103	64-65	-0.1527670086303914
1103	66-67	-0.0948442371749394
1103	68-69	-0.28795931300520294
1103	70-71	-0.2144053077696455
1103	72-73	-0.2532734514699939
1103	74-75	-0.2373755722523967
1103	76-77	-0.2312970491697044
1103	78-79	-0.2577585830019302
1103	80-81	-0.19192899858056123
1103	82-83	-0.2420324469197439
1103	84-85	-0.27016736615446035
1103	86-87	-1.057246657532712
1103	88-89	-0.5712061148191481
1103	90-91	-0.8367038156478834
1103	92-93	-0.4433059995289099
1103	94-95	-0.786799734247051
1103	96-97	-0.6124816479533415
1103	98-99	-0.49109695722094315
1103	100	-0.4837646604794763
1104	1	0.031335903171839874
1104	2	-0.00550418293429189
1104	3	0.020761925878016285
1104	4	0.025272373749018584
1104	5	-0.03348055501431446
1104	6	0.09394148964792493
1104	7	0.018059529391265983
1104	8	0.07075405793645473
1104	9	0.06958644455338003
1104	10-11	-0.08431884615975349
1104	12-13	0.05448914295076435
1104	14-15	-0.07095225509051772
1104	16-17	-0.020802305897987594
1104	18-19	0.04561270334600209
1104	20-21	-0.07105191679900713
1104	22-23	-0.026723403033827253
1104	24-25	-0.0386062472810309
1104	26-27	-0.03136543246952783
1104	28-29	-0.04865134616449751
1104	30-31	-0.0165693450419937
1104	32-33	0.04243384302355224
1104	34-35	-0.1270572520187585
1104	36-37	-0.11047079746357724
1104	38-39	-0.0699277027403653
1104	40-41	0.01800427498724133
1104	42-43	-0.25872288343806105
1104	44-45	-0.1668906789545943
1104	46-47	-0.04965758240589935
1104	48-49	-0.0631494440382312
1104	50-51	-0.2317371604525178
1104	52-53	-0.21782188575119932
1104	54-55	-0.02486698000304699
1104	56-57	0.009639737360728873
1104	58-59	-0.00525584435784765
1104	60-61	-0.09618635234777884
1104	62-63	0.06339246104041507
1104	64-65	0.0032881222551779388
1104	66-67	-0.07138141744824367
1104	68-69	-0.08680568311517334
1104	70-71	-0.014979851773240682
1104	72-73	0.002176376439550154
1104	74-75	0.003239496119711305
1104	76-77	-0.026637344626202264
1104	78-79	-0.14472147160155657
1104	80-81	-0.07085425784092081
1104	82-83	-0.10756806366066485
1104	84-85	-0.14497482679068696
1104	86-87	-0.17188712456465538
1104	88-89	-0.08428053633248922
1104	90-91	-0.26630170854830837
1104	92-93	-0.23088567456561293
1104	94-95	-0.18583381788328523
1104	96-97	-0.22625271954761317
1104	98-99	-0.294084419981818
1104	100	-0.24257429158727817
1105	1	0.16520685052544337
1105	2	0.09295752424514703
1105	3	0.14405193334300392
1105	4	0.07992646272435167
1105	5	0.12909867340026437
1105	6	0.18198003192132717
1105	7	0.04348200687491044
1105	8	0.044440103862910973
1105	9	0.13872034394415778
1105	10-11	0.14489846474296542
1105	12-13	0.19150403035278174
1105	14-15	0.11809773001357371
1105	16-17	0.05582675173604201
1105	18-19	0.07570269696395115
1105	20-21	0.05738167885367318
1105	22-23	0.12335561739289602
1105	24-25	0.06813829289573903
1105	26-27	0.0433447038533501
1105	28-29	0.03417453929277059
1105	30-31	-0.032043893301711535
1105	32-33	0.05777104619920692
1105	34-35	0.02107800754971123
1105	36-37	-0.03177883686221605
1105	38-39	0.003228040954752487
1105	40-41	-0.08410038330637093
1105	42-43	-0.08267141701163183
1105	44-45	-0.1267003393461721
1105	46-47	-0.13866752912476699
1105	48-49	-0.0982931873286006
1105	50-51	-0.18954898824247834
1105	52-53	-0.06310942282021159
1105	54-55	0.017162512855477985
1105	56-57	0.06378733212725507
1105	58-59	0.031231899984369704
1105	60-61	0.10007142266675828
1105	62-63	-0.009892600614399782
1105	64-65	0.014664036447236128
1105	66-67	0.13971739877836598
1105	68-69	0.1262894051666379
1105	70-71	0.13615351325108804
1105	72-73	0.03443985082799372
1105	74-75	0.0376786340036972
1105	76-77	0.11993137793150765
1105	78-79	0.1131910070124178
1105	80-81	0.06869758845106233
1105	82-83	0.08670461811482255
1105	84-85	0.027287360945926764
1105	86-87	0.03214171261957688
1105	88-89	-0.013867325468662983
1105	90-91	-0.0663607821999328
1105	92-93	-0.0656322883719831
1105	94-95	-0.01672303973860778
1105	96-97	-0.054641198069457886
1105	98-99	-0.0666217990293525
1105	100	-0.11645860034609967
1106	1	0.02359684172251164
1106	2	0.04514703447150836
1106	3	0.11324221315768312
1106	4	0.1008737797248429
1106	5	0.09997973326541398
1106	6	0.15973100564711729
1106	7	0.14878468217718677
1106	8	-0.9921780761909815
1106	9	0.163678174929899
1106	10-11	0.13333794967841328
1106	12-13	0.11570148602194053
1106	14-15	0.057558951200618935
1106	16-17	0.09922104086697203
1106	18-19	0.14075081128986966
1106	20-21	0.11304981894622301
1106	22-23	0.13886863465479848
1106	24-25	0.11596502975939416
1106	26-27	0.07632536954554325
1106	28-29	0.03228032212676624
1106	30-31	0.06391663757009525
1106	32-33	0.029260157299326295
1106	34-35	0.127042432039012
1106	36-37	0.1328101499926646
1106	38-39	0.04591166030837002
1106	40-41	0.11156668492484556
1106	42-43	0.1342652134501705
1106	44-45	0.007418191058860657
1106	46-47	0.04430164315535734
1106	48-49	0.021425129678583232
1106	50-51	0.046891556509393695
1106	52-53	0.08541284310276609
1106	54-55	0.14654706009319796
1106	56-57	0.0667328463165262
1106	58-59	0.1733703113870817
1106	60-61	0.16632348628741056
1106	62-63	0.061083685062634174
1106	64-65	0.13012698276261148
1106	66-67	0.12748747789107284
1106	68-69	0.1681147302048629
1106	70-71	0.07432667718612151
1106	72-73	0.11659001345790898
1106	74-75	0.141726740953807
1106	76-77	0.10876542828638947
1106	78-79	0.11436347714730744
1106	80-81	0.12739221427773728
1106	82-83	0.16079455269406395
1106	84-85	0.11050099745812503
1106	86-87	0.0769080891415932
1106	88-89	0.0971110247594531
1106	90-91	0.06950888474013439
1106	92-93	0.11219834413920893
1106	94-95	0.001736073657490067
1106	96-97	-0.008232373375559376
1106	98-99	0.024189608796223894
1106	100	0.01815893429028037
1107	1	-0.08447740022139882
1107	2	-0.11539530665792341
1107	3	-0.1594059077959038
1107	4	0.10425912963895456
1107	5	0.06442066947869307
1107	6	0.050964713200649214
1107	7	0.1247648641314143
1107	8	0.1661726186277761
1107	9	0.09485072533676231
1107	10-11	0.16675910191674603
1107	12-13	0.0969198790018595
1107	14-15	0.08570330320131347
1107	16-17	0.13586293164919283
1107	18-19	0.1351583833729677
1107	20-21	0.14392188722417387
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2310	2	0.20514592367777595
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2310	4	0.26915211798510796
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2310	6	0.23500439767229864
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2310	8	0.2824566030327347
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2311	3	0.10870768684830168
2311	4	0.025640383867127525
2311	5	-2.466594935270905E-4
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2312	56-57	-1.2539447573954376
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2312	68-69	0.26423449097218565
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2312	80-81	0.4371790409266332
2312	82-83	0.3960848680410649
2312	84-85	0.4024163050712275
2312	86-87	0.5270497703124448
2312	88-89	0.5240901985627495
2312	90-91	0.5551127924702222
2312	92-93	0.6760692081960933
2312	94-95	0.6601289407085815
2312	96-97	0.5670637255539681
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2313	1	0.09506158688597566
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2313	4	0.12389909535529853
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2313	64-65	0.1836461045183242
2313	66-67	0.23005426758592762
2313	68-69	0.16907098333660286
2313	70-71	0.2079514190489533
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2313	80-81	0.1537574634484109
2313	82-83	0.3029188692230669
2313	84-85	0.29737205532525834
2313	86-87	0.4237810114858078
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2313	92-93	0.49121456792433094
2313	94-95	0.4725802660475864
2313	96-97	0.6052169785139405
2313	98-99	0.553274285975558
2313	100	0.6335873876593006
2314	1	-0.5009835752541214
2314	2	-0.3215311921464057
2314	3	-0.3166139385197191
2314	4	-0.3125170280425351
2314	5	-0.30785602020237945
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2314	10-11	-0.25737974411259046
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2314	14-15	-0.18045173156678374
2314	16-17	-0.15065471401393182
2314	18-19	-9.233297388320238E-4
2314	20-21	-0.030985913375630503
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2314	24-25	0.033276579891669655
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2314	54-55	0.048780155475377285
2314	56-57	-0.031357900020864804
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2314	78-79	0.1524324828127419
2314	80-81	0.14019100555330866
2314	82-83	0.14749606145196736
2314	84-85	0.22287641240276912
2314	86-87	0.30847337050335
2314	88-89	0.3727233484723165
2314	90-91	0.3533981420168679
2314	92-93	0.4238965615515191
2314	94-95	0.4180448816795277
2314	96-97	0.5430680957237897
2314	98-99	0.5723915111177256
2314	100	0.4331916151916566
2315	1	-0.020829893082733975
2315	2	-0.04534946003811413
2315	3	-0.0016936403620775309
2315	4	0.022646390866633226
2315	5	-0.002997743830050581
2315	6	-0.05830904765002032
2315	7	-0.06143053958339806
2315	8	0.020530467142606312
2315	9	-0.10175876693899255
2315	10-11	-1.4507149797764924
2315	12-13	-0.009125139856692499
2315	14-15	-0.040255050915810386
2315	16-17	-0.08964980425846392
2315	18-19	-0.044720497734950015
2315	20-21	-0.02529652910630631
2315	22-23	-0.05044478491547011
2315	24-25	-0.037426585383094846
2315	26-27	-0.023940825240714503
2315	28-29	0.01798710653333302
2315	30-31	0.04524191473564798
2315	32-33	-0.008727410875458474
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2315	90-91	0.4036708578043253
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2315	94-95	0.4139741120778986
2315	96-97	0.5477289871471847
2315	98-99	0.601449066056599
2315	100	0.6275263004866396
2316	1	-0.24438260172587079
2316	2	-0.41210108736927964
2316	3	-0.2933851924789792
2316	4	-0.31738916218691315
2316	5	-0.26519526031964347
2316	6	-0.15446166648666804
2316	7	-0.11916809373450832
2316	8	-0.13160890756687138
2316	9	-0.08514752758711808
2316	10-11	-0.3244589540404448
2316	12-13	-0.2713420213650366
2316	14-15	-0.3075315678917363
2316	16-17	-0.36787776292331387
2316	18-19	-0.25506875315884514
2316	20-21	-0.13325690987667826
2316	22-23	-0.18604684361430657
2316	24-25	-0.1910942715612194
2316	26-27	-0.26085424053400175
2316	28-29	-0.11454996967554365
2316	30-31	-0.16938068556095942
2316	32-33	-0.1819510728954299
2316	34-35	-0.21485466780023899
2316	36-37	-0.08653432618044832
2316	38-39	-0.22178044263663566
2316	40-41	-0.1342159203587059
2316	42-43	-0.143500989219838
2316	44-45	-0.08948606316275232
2316	46-47	-1.520800981512231
2316	48-49	-0.22577758176871754
2316	50-51	0.01242786561397935
2316	52-53	-0.05667655398794125
2316	54-55	-0.1356804879382807
2316	56-57	-0.18648163739328538
2316	58-59	-0.369557916408354
2316	60-61	-0.230450730626103
2316	62-63	-0.19986823892392636
2316	64-65	-0.3761462497698673
2316	66-67	-0.30247671177671265
2316	68-69	-0.06770555352412089
2316	70-71	-0.05882077489261661
2316	72-73	-0.1461007044631657
2316	74-75	-0.06951553418387135
2316	76-77	-0.1945095895979918
2316	78-79	-0.3168091852642263
2316	80-81	-0.17342522067305666
2316	82-83	-0.09484932219348963
2316	84-85	0.03878255625815541
2316	86-87	0.07827630887000225
2316	88-89	0.05719799216925736
2316	90-91	0.1824356802734428
2316	92-93	0.37049405172393435
2316	94-95	0.3084074423092886
2316	96-97	0.3775808057286305
2316	98-99	0.33979161678489334
2316	100	0.055300426294238036
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	22361.0
1	2020.0
2	1337.0
3	945.0
4	820.0
5	747.0
6	854.0
7	1295.0
8	2302.0
9	4124.0
10	7504.0
11	14667.0
12	24368.0
13	32135.0
14	37743.0
15	43394.0
16	49279.0
17	56088.0
18	64211.0
19	73920.0
20	84252.0
21	95441.0
22	108150.0
23	121656.0
24	136916.0
25	156987.0
26	183058.0
27	212376.0
28	251269.0
29	301193.0
30	364764.0
31	451373.0
32	575655.0
33	766360.0
34	1107079.0
35	1736730.0
36	1780464.0
37	1809978.0
38	1415377.0
39	201987.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.952868823264154	16.685862250794926	11.989043252303734	25.37222567363719
2	16.726194040147135	19.030881235080194	39.18539316702115	25.057531557751535
3	20.54805613569252	24.14818270114488	27.228175818332907	28.075585344829694
4	14.064789720620752	14.772244840975295	34.65459361839649	36.50837182000746
5	15.222561005693535	35.86457022681259	32.514683176835916	16.39818559065796
6	34.07746706863853	32.77946461494523	17.97737066190056	15.165697654515679
7	30.9105694582283	29.70225085061405	20.390578234974225	18.99660145618343
8	27.981265191324393	33.43439894947618	19.573732894490284	19.01060296470914
9	28.44462972059512	15.334759749564174	18.560059226053983	37.66055130378672
10-11	27.11931945404577	24.430720408004845	26.668651110177976	21.78130902777141
12-13	27.110947648326345	21.669882378111836	26.686225147345738	24.53294482621608
14-15	24.627518630485266	23.171143792870772	23.992879849269418	28.20845772737454
16-17	24.98237376995317	25.804980065980608	24.69566028037033	24.51698588369589
18-19	24.964529839698958	25.511868735162018	25.51746783907719	24.006133586061836
20-21	25.47530047991453	24.953131038180157	25.244151582851586	24.327416899053723
22-23	25.300044854334246	24.656343595935468	24.981737179679424	25.061874370050862
24-25	25.051620027213666	24.84870940458158	24.955500608829663	25.14416995937509
26-27	24.92826815236355	24.836970665782623	25.203069267801574	25.031691914052256
28-29	24.910218151746818	25.069738410004593	25.19246040712895	24.827583031119634
30-31	25.554530814058314	24.88767060774505	25.039218262808504	24.51858031538813
32-33	25.173601984763174	24.941305442589197	25.101820499097098	24.783272073550535
34-35	25.228220792106313	25.092447062750246	25.127840964650268	24.551491180493173
36-37	25.32213478209741	24.969058843653585	25.015640696468783	24.69316567778022
38-39	25.271208974544113	25.162263312013337	24.860057978308824	24.706469735133727
40-41	25.157704019392874	24.904591029570007	25.128833643752362	24.80887130728476
42-43	25.014588048981288	24.87062682483046	24.996976443479298	25.117808682708958
44-45	24.878504244146892	24.92545205963118	25.168832664141245	25.027211032080682
46-47	24.876894969183862	24.733056320105963	25.18330637232962	25.206742338380554
48-49	24.931866246891385	24.81601961316515	25.273367290835395	24.97874684910807
50-51	24.69512849775053	24.958345354809815	25.38894679887011	24.95757934856955
52-53	24.724670947708823	24.998637867511395	25.13872662271954	25.137964562060244
54-55	24.553917435299287	24.792147934484554	25.429659972250935	25.22427465796523
56-57	24.695561500970115	24.849033944397476	25.201957802699106	25.2534467519333
58-59	24.70695392239368	24.8318991602803	25.403422046258957	25.057724871067062
60-61	25.010917707580475	24.865249126237057	25.395211235537346	24.728621930645115
62-63	24.720342920215572	24.91098230821647	25.400302977035278	24.96837179453268
64-65	24.600238503238735	24.727058225445756	25.446908291425185	25.225794979890324
66-67	24.692592901135598	24.777175195106203	25.262077542966892	25.26815436079131
68-69	24.425872016897458	24.74014377201183	25.439896291054463	25.394087920036252
70-71	24.424738210690737	24.541204973798138	25.58905524700726	25.445001568503873
72-73	24.410590037826804	24.768211947530087	25.35553854069582	25.465659473947287
74-75	24.35906361684763	24.80236441221618	25.47849248870226	25.36007948223393
76-77	24.181562343363005	24.544706432339115	25.612580190702104	25.661151033595768
78-79	24.135992005917632	24.492340227180502	25.742475344976317	25.629192421925556
80-81	24.230819922158716	24.7706766368803	25.419651504942088	25.578851936018904
82-83	24.18918374186883	24.687966060874228	25.54636890343823	25.576481293818716
84-85	24.165626226327884	24.75114116898603	25.292456844570175	25.79077576011591
86-87	24.01466767120639	24.697280780971482	25.665330939698432	25.622720608123693
88-89	23.84051634053988	24.495827177526955	25.80444328458542	25.859213197347742
90-91	24.25686333901259	24.3800232819779	25.73564271575684	25.627470663252673
92-93	24.151423645957383	24.31756776967825	25.787802056977462	25.743206527386903
94-95	23.98745379897219	24.197020470872626	25.855732954676075	25.95979277547911
96-97	23.956679849901153	24.10263985476472	25.9368796857741	26.003800609560024
98-99	23.94695754769847	24.053621171335475	25.845844736203972	26.153576544762085
100	24.044469420893595	23.9808778522551	25.857799306150763	26.116853420700544
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6354.0
1	8682.5
2	7898.5
3	2874.5
4	842.0
5	684.0
6	616.0
7	554.0
8	516.0
9	517.0
10	511.0
11	530.5
12	582.0
13	624.0
14	681.5
15	802.0
16	933.0
17	1020.5
18	1151.0
19	1373.0
20	1563.0
21	1733.5
22	1990.0
23	2364.0
24	2884.0
25	3585.5
26	4840.5
27	6766.5
28	9536.0
29	13765.5
30	20127.0
31	28913.0
32	41639.5
33	58769.0
34	79164.5
35	104400.0
36	140590.0
37	177442.5
38	209726.0
39	242449.5
40	269727.5
41	296096.0
42	326582.5
43	361326.0
44	396506.5
45	431281.5
46	467346.5
47	504949.0
48	547854.0
49	586156.0
50	622058.0
51	628983.5
52	603460.0
53	591349.0
54	583771.5
55	558721.5
56	517082.5
57	472811.5
58	432929.0
59	381563.0
60	320235.5
61	265864.0
62	220023.0
63	176156.5
64	133630.0
65	100253.5
66	75615.5
67	58589.0
68	45152.5
69	33877.0
70	26443.0
71	20522.5
72	15678.0
73	12119.0
74	9363.5
75	6746.0
76	4657.5
77	3010.5
78	1869.5
79	1221.0
80	791.0
81	515.0
82	372.5
83	242.5
84	163.0
85	149.0
86	123.0
87	89.5
88	74.5
89	62.0
90	43.0
91	36.5
92	37.0
93	28.0
94	19.0
95	15.0
96	16.5
97	14.5
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14635145648273626
2	0.07174903932214799
3	0.31525354009890083
4	0.3131724440116054
5	0.314660102542758
6	0.2630472937215119
7	0.2636000848696998
8	0.2624375976021871
9	0.27343651559480736
10-11	0.2501176713510297
12-13	0.26031991974773216
14-15	0.23344857944868838
16-17	0.2416510245740053
18-19	0.25517408450063045
20-21	0.2571251120824699
22-23	0.24695131617133598
24-25	0.24823980730350917
26-27	0.24660582170371856
28-29	0.256954397169059
30-31	0.24243550024753663
32-33	0.25471478009073906
34-35	0.23836679481124215
36-37	0.24661801562610505
38-39	0.24724397030861192
40-41	0.264839800312327
42-43	0.25112976690910893
44-45	0.23634666833587917
46-47	0.23959838097227837
48-49	0.24937790672624888
50-51	0.24200464832321375
52-53	0.2588810369061255
54-55	0.2686686919416871
56-57	0.26467315003971154
58-59	0.26204739208581923
60-61	0.24274847758879006
62-63	0.27057094383398056
64-65	0.26512432516801193
66-67	0.2705099742220481
68-69	0.2747819116981175
70-71	0.29539776981288834
72-73	0.3041977171351436
74-75	0.28306971428013994
76-77	0.2812040441550059
78-79	0.2775011563903063
80-81	0.2633805942667429
82-83	0.2750501779906205
84-85	0.2859027689146027
86-87	0.2782815674230421
88-89	0.28738229816416433
90-91	0.2816023789529648
92-93	0.2604865700203476
94-95	0.28215110546035715
96-97	0.2804073745590881
98-99	0.2661120328813182
100	0.2880448346138307
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.230121E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.223794981758495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.866804018634205	28.24432447060229
2	17.615517035949146	15.580500277909785
3	7.704890653660936	10.222185138731051
4	3.7942603329078777	6.71186364279747
5	2.100178114542412	4.643892318134987
6	1.218967765126164	3.2344428320587086
7	0.8058606779387798	2.494675218351786
8	0.5425257687692544	1.919403869629794
9	0.3922083562786024	1.5610447744377667
>10	1.776114923380823	13.661700531610926
>50	0.11056754054916226	3.364113589601246
>100	0.06577687482794706	5.483251925433332
>500	0.0047224632686386535	1.3837139295147558
>1k	0.0014795546257508141	1.0594059168862457
>5k	9.44396551877041E-5	0.2369873939156791
>10k+	3.147988506256803E-5	0.19849417038417158
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	14258	0.1159072969244489	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.690497113698571E-5
2	0.0	0.0	0.0	0.0	7.316353431898163E-5
3	0.0	0.0	0.0	0.0	7.316353431898163E-5
4	0.0	0.0	0.0	0.0	7.316353431898163E-5
5	0.0	0.0	0.0	0.0	7.316353431898163E-5
6	0.0	0.0	0.0	0.0	8.129281590997959E-5
7	0.0	0.0	0.0	0.0	8.129281590997959E-5
8	0.0	0.0	0.0	0.0	1.0568066068297346E-4
9	0.0	0.0	0.0	0.0	1.4632706863796326E-4
10-11	0.0	0.0	0.0	0.0	1.5039170943346224E-4
12-13	0.0	0.0	0.0	0.0	1.585209910244602E-4
14-15	0.0	4.064640795498979E-6	0.0	0.0	1.6665027261545816E-4
16-17	0.0	8.129281590997959E-6	0.0	4.064640795498979E-6	2.4387844772993876E-4
18-19	0.0	8.129281590997959E-6	0.0	1.6258563181995917E-5	2.763955740939306E-4
20-21	0.0	8.129281590997959E-6	0.0	2.0323203977494897E-5	2.8858949648042753E-4
22-23	0.0	8.129281590997959E-6	0.0	3.6581767159490814E-5	3.576883900039102E-4
24-25	0.0	8.129281590997959E-6	0.0	1.0161601988747449E-4	4.2272264273189385E-4
26-27	0.0	8.129281590997959E-6	0.0	2.3168452534344183E-4	4.471104875048877E-4
28-29	0.0	8.129281590997959E-6	0.0	9.714491501242561E-4	4.471104875048877E-4
30-31	4.064640795498979E-6	8.129281590997959E-6	0.0	0.0032842297627631756	4.471104875048877E-4
32-33	8.129281590997959E-6	8.129281590997959E-6	0.0	0.011194020750804189	4.918215362553765E-4
34-35	8.129281590997959E-6	8.129281590997959E-6	0.0	0.02832648170383239	5.85308274551853E-4
36-37	8.129281590997959E-6	2.0323203977494897E-5	0.0	0.06312387155409915	5.93437556142851E-4
38-39	8.129281590997959E-6	3.2517126363991835E-5	0.0	0.1401935256775553	6.422132456888387E-4
40-41	8.129281590997959E-6	3.2517126363991835E-5	0.0	0.24637007253757964	6.706657312573316E-4
42-43	8.129281590997959E-6	3.2517126363991835E-5	0.0	0.352522231552831	6.869242944393275E-4
44-45	8.129281590997959E-6	3.2517126363991835E-5	0.0	0.4869602258639597	7.072474984168224E-4
46-47	8.129281590997959E-6	3.2517126363991835E-5	0.0	0.651078227263822	7.316353431898163E-4
48-49	8.129281590997959E-6	3.2517126363991835E-5	0.0	0.8266991621149464	8.04798877508798E-4
50-51	8.129281590997959E-6	3.2517126363991835E-5	0.0	1.016668278974182	8.04798877508798E-4
52-53	8.129281590997959E-6	3.2517126363991835E-5	0.0	1.2154454724372643	8.698331302367816E-4
54-55	8.129281590997959E-6	4.0646407954989794E-5	0.0	1.4465528187877452	9.470613053512622E-4
56-57	8.129281590997959E-6	4.0646407954989794E-5	0.0	1.7148272405722689	9.755137909197549E-4
58-59	8.129281590997959E-6	4.0646407954989794E-5	0.0	2.0094445993524213	0.001003966276488248
60-61	8.129281590997959E-6	4.0646407954989794E-5	0.0	2.3167761545408947	0.0010242894804657428
62-63	8.129281590997959E-6	4.0646407954989794E-5	0.0	2.6396630900537428	0.0010405480436477387
64-65	8.129281590997959E-6	4.0646407954989794E-5	0.0	2.9687567320613173	0.0010446126844432376
66-67	8.129281590997959E-6	4.0646407954989794E-5	0.0	3.341760688582668	0.0010974530147847244
68-69	8.129281590997959E-6	4.0646407954989794E-5	0.0	3.772844297430903	0.0011380994227397142
70-71	8.129281590997959E-6	4.877568954598775E-5	0.0	4.245988809230962	0.001158422626717209
72-73	8.129281590997959E-6	5.690497113698571E-5	0.0	4.694269913284954	0.0013047496953551723
74-75	8.129281590997959E-6	6.503425272798367E-5	0.0	5.143144454895088	0.0013088143361506713
76-77	8.129281590997959E-6	6.503425272798367E-5	0.0	5.63126716802656	0.00135352538490116
78-79	8.129281590997959E-6	6.503425272798367E-5	0.0	6.175384372756826	0.0013982364336516488
80-81	8.129281590997959E-6	6.503425272798367E-5	0.0	6.746824092914437	0.0015039170943346224
82-83	1.2193922386496938E-5	6.503425272798367E-5	0.0	7.33885934798284	0.0016908905709275754
84-85	1.6258563181995917E-5	6.503425272798367E-5	0.0	7.939247439885995	0.0016908905709275754
86-87	1.6258563181995917E-5	6.503425272798367E-5	0.0	8.556922449092406	0.0016990198525185735
88	1.6258563181995917E-5	6.503425272798367E-5	0.0	9.044191587656824	0.0017315369788825652
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	20100	0.0	50.719994	2
GTACCTG	24980	0.0	41.254253	1
ACCTGGG	24620	0.0	40.62519	3
CCTGGGG	24070	0.0	29.633137	4
TGGGGGG	18170	0.0	25.474873	6
CATGGGG	37095	0.0	25.223528	4
GTACATG	62405	0.0	24.653574	1
ATGGGGG	21590	0.0	24.346472	5
TACATGG	64090	0.0	23.838306	2
ACATGGG	65240	0.0	22.704533	3
CTGGGGG	14435	0.0	21.56195	5
TATACCG	1055	0.0	19.60862	5
TATCACG	990	0.0	19.474424	2
GTATCAA	39545	0.0	18.756804	1
GTACACG	2960	0.0	17.002424	1
CAACGCA	42635	0.0	16.949404	5
TCAACGC	42630	0.0	16.930162	4
AACGCAG	44470	0.0	16.411001	6
ATCAACG	44320	0.0	16.27577	3
TATCAAC	44700	0.0	16.232088	2
>>END_MODULE