FastQCFastQC Report
Mon 6 Jun 2016
ERR522924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12301210
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA140130.1139156229346544No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG208000.050.3432852
ACCTGGG251650.040.895793
GTACCTG259450.040.7400251
CCTGGGG244850.030.42014
TGGGGGG167000.027.9706866
CATGGGG366400.024.3428944
ATGGGGG209400.023.4515235
GTACATG627450.023.208161
CTGGGGG143050.023.1925375
TACATGG647500.022.291012
ACATGGG650950.021.6645573
TATCACG11950.019.6649042
GTACACG29750.018.8078291
GTATACG7600.017.9416771
GTATATA28050.017.6009081
GTATCAA388350.017.3985121
GTACCCG25400.017.030711
CAACGCA420250.015.6549535
TCAACGC420300.015.6083684
TACCGTA11250.015.4554777
Produced by FastQC (version 0.11.5)