FastQCFastQC Report
Mon 6 Jun 2016
ERR522923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727851
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97970.3591471821591429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79130.29008182631675994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44810.16426850293509432No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27540.10095859341291001No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG37100.046.0104682
ACCTGGG39750.041.8791663
GTACCTG48350.035.7904661
GGTATCA83700.028.5400181
GTATCAA119300.027.276061
CCTGGGG42350.026.9826584
ATAACGC3200.024.982213
TATAACG2701.8189894E-1224.383062
TCAACGC134900.024.052994
TAACGCA3150.023.8858854
CAACGCA135700.023.8414425
ATCAACG136250.023.7801533
GTACATG92800.023.7651671
AACGCAG140550.023.213526
TATCAAC140100.023.1598052
TACATGG94050.022.8497452
ACATGGG94400.022.217483
CATGGGG58650.022.129574
ACGCAGA153950.021.250947
GTATAAC6900.021.1265491
Produced by FastQC (version 0.11.5)