FastQCFastQC Report
Mon 6 Jun 2016
ERR522923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727851
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93510.3427973155425278No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80840.2963504971495877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44060.16151908590315234No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT35130.128782693776163No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27960.10249826695079753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG42000.047.224622
ACCTGGG44750.043.6923683
GTACCTG53400.037.3388441
TAACGCA2550.035.0202064
CCTGGGG42150.031.7799034
TATCACG2750.029.0550422
GTATCAA116650.027.2923031
TATAACG2600.027.1158492
GGTATCA82750.026.8231771
ATAACGC2950.025.4919623
TCAACGC133150.023.4386314
CAACGCA134700.023.1693465
ATCAACG135050.023.1436773
GTACATG86750.022.7675361
TATCAAC138700.022.670182
AACGCAG139650.022.5483766
TACATGG88200.022.0616042
ACATGGG88350.020.9601783
CTGGGGG24200.020.781735
ACGCAGA154950.020.2309257
Produced by FastQC (version 0.11.5)