FastQCFastQC Report
Mon 6 Jun 2016
ERR522922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7691726
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT779731.0137256579342528No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT235480.3061471508475471No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234880.3053670918594864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182290.23699492155596805No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC179480.23334164529521723No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC165180.21475023941310442No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA133290.17329010419767943No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG130170.1692337974597639No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA112520.14628706222764565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101420.13185597094852314No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG312150.028.3936751
TACATGG316700.027.6276252
ACATGGG327800.025.8467083
CATGGGG238500.024.6603934
ATGGGGG156250.022.7779925
GAGTACT197150.020.62667812-13
GTATAGG20150.019.8343281
TGGGGGG146500.019.0821386
GTACTTT212900.018.91211514-15
AGAGTAC348500.018.76635410-11
TACCTGG52700.018.3781532
AGTACTT208850.017.87386312-13
GAGTACA241150.017.0411051
AGTACAT234500.016.0796572
ACTTTTT257700.015.80576716-17
CATGGGA102950.015.663864
TACTTTT243650.015.17550814-15
TACCGTA4101.2376404E-814.8968987
ATGGGGA82200.014.8710325
GTACCTG66950.014.8185421
Produced by FastQC (version 0.11.5)