##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522922_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7691726
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.933422745428008	34.0	31.0	34.0	30.0	34.0
2	32.239175836476754	34.0	31.0	34.0	30.0	35.0
3	32.087848683117414	34.0	31.0	34.0	28.0	35.0
4	34.702272415840085	35.0	35.0	37.0	32.0	37.0
5	34.709840808161914	37.0	34.0	37.0	32.0	37.0
6	34.88410234581939	37.0	35.0	37.0	33.0	37.0
7	34.736934857013885	37.0	35.0	37.0	32.0	37.0
8	34.87761290508788	37.0	35.0	37.0	33.0	37.0
9	36.12966985563448	39.0	35.0	39.0	32.0	39.0
10-11	36.229231904516624	38.0	35.5	39.0	33.0	39.0
12-13	36.27698529562805	38.0	35.5	39.0	32.5	39.0
14-15	37.3595027956014	39.0	36.5	41.0	32.5	41.0
16-17	37.25280125683104	39.0	36.5	41.0	32.0	41.0
18-19	37.27985337751241	39.0	36.5	41.0	32.5	41.0
20-21	37.30069882884544	39.0	36.0	41.0	32.0	41.0
22-23	37.21001892163085	39.0	36.0	41.0	32.0	41.0
24-25	37.20375979331557	39.0	36.5	41.0	32.0	41.0
26-27	37.0217275680387	39.0	36.5	41.0	32.0	41.0
28-29	36.837046522458024	39.0	36.0	41.0	31.0	41.0
30-31	36.75713831979974	38.5	36.0	41.0	30.5	41.0
32-33	36.58687926481001	38.0	35.0	41.0	30.5	41.0
34-35	36.481068358389265	38.5	35.0	41.0	30.0	41.0
36-37	36.366429134371145	38.0	35.5	40.5	30.5	41.0
38-39	36.125488934473225	38.0	35.5	40.0	30.0	41.0
40-41	36.08901877159951	38.0	35.0	40.0	30.0	41.0
42-43	36.046297801039714	38.0	35.0	40.0	29.0	41.0
44-45	35.941282749801545	38.0	35.0	40.0	29.0	41.0
46-47	35.6561709296457	38.0	35.0	40.0	28.0	41.0
48-49	35.762781851563616	37.5	35.0	40.0	28.0	41.0
50-51	34.73948701500808	37.5	34.0	39.0	26.0	40.0
52-53	34.91144952381299	37.0	34.5	39.5	27.5	40.0
54-55	35.498989238566224	37.0	35.0	40.0	28.0	41.0
56-57	35.63255802403778	37.0	35.0	40.0	28.0	41.0
58-59	35.61498427011051	38.0	35.0	40.0	28.0	41.0
60-61	35.32454718225792	37.0	35.0	40.0	28.0	41.0
62-63	35.11579572647284	37.0	34.0	40.0	27.0	41.0
64-65	34.88042553777917	36.5	34.0	39.5	26.5	41.0
66-67	34.622882627384286	37.0	34.0	39.0	26.0	41.0
68-69	34.26686851559715	36.0	34.0	39.0	26.0	41.0
70-71	33.84716694276421	36.0	34.0	38.0	26.0	40.0
72-73	33.58421445329696	35.5	33.5	37.5	25.5	39.5
74-75	33.33219221537533	35.0	33.5	37.0	25.0	39.0
76-77	32.99914356023602	35.0	33.0	36.5	24.5	39.0
78-79	32.69729069912267	35.0	33.0	37.0	24.0	37.5
80-81	32.46903217821331	35.0	32.0	36.0	23.0	37.5
82-83	32.24851801793251	35.0	32.0	36.0	23.0	37.5
84-85	31.964215053942375	35.0	32.0	35.5	21.5	37.0
86-87	31.89572749991354	35.0	32.0	36.0	21.0	37.0
88-89	31.59143025895618	35.0	32.0	35.0	19.5	37.0
90-91	31.48938164203977	35.0	31.0	35.0	20.0	36.5
92-93	31.252386655478887	35.0	31.0	35.0	19.0	36.0
94-95	31.155319170235654	34.5	31.0	35.0	18.5	36.0
96-97	30.8911861914998	34.0	30.5	35.0	19.0	36.0
98-99	30.853424445956605	34.5	30.5	35.0	18.5	36.5
100	29.601037530458054	34.0	29.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.026658054228608563
1101	2	0.08241741341391418
1101	3	0.022262401716986346
1101	4	0.07220749146830485
1101	5	0.06843239360630804
1101	6	0.10332591428430504
1101	7	0.16796871145704984
1101	8	0.14340838026127756
1101	9	-0.027559342441378476
1101	10-11	0.09499238938052201
1101	12-13	0.04896948499943932
1101	14-15	0.09950302960415058
1101	16-17	0.036688829309056814
1101	18-19	0.08570008480208458
1101	20-21	0.08296137276674642
1101	22-23	0.0786791458694438
1101	24-25	-0.0489978481461506
1101	26-27	-0.04817867778042029
1101	28-29	-0.04937720522357125
1101	30-31	-0.01778484875865871
1101	32-33	-0.025398623909381968
1101	34-35	-0.13632809153886427
1101	36-37	-0.07497525099018532
1101	38-39	-0.09659184101931118
1101	40-41	-0.09451886458254677
1101	42-43	-0.032050006531115116
1101	44-45	-0.07739799819076154
1101	46-47	-0.10744851403651268
1101	48-49	-0.21351008993102738
1101	50-51	-0.33405587784567814
1101	52-53	-0.2960627375442968
1101	54-55	-0.10015533415496236
1101	56-57	-0.060738461125652066
1101	58-59	-0.07564929534038356
1101	60-61	-0.05408794965327246
1101	62-63	-0.05293891802325845
1101	64-65	-0.027099798629691918
1101	66-67	-0.3489086411562994
1101	68-69	-0.08612392126671153
1101	70-71	-0.18286115623861576
1101	72-73	-0.18601800700374582
1101	74-75	-0.16059081286394417
1101	76-77	-0.19606927475894764
1101	78-79	-0.4805650920501776
1101	80-81	-0.208593073696683
1101	82-83	-0.19632461018061775
1101	84-85	-0.5426933046769058
1101	86-87	-0.8482924185628384
1101	88-89	-0.39254626865760756
1101	90-91	-0.6154220400725734
1101	92-93	-0.3325968139406932
1101	94-95	-0.5761883678779149
1101	96-97	-0.5127076727668651
1101	98-99	-0.5147492471079822
1101	100	-0.6355715690795023
1102	1	-0.05612668697686729
1102	2	-0.06571377657743227
1102	3	0.05490972796915372
1102	4	0.0029090616671041403
1102	5	-3.8957198560041206E-4
1102	6	-0.011491850642855184
1102	7	0.1305175537853529
1102	8	-0.006750203086468787
1102	9	-0.07096832121045793
1102	10-11	-0.014997903948419378
1102	12-13	-0.0866187855376026
1102	14-15	-0.05746853521231543
1102	16-17	-0.13092596475583207
1102	18-19	-0.0669726537868911
1102	20-21	-0.0903482496840411
1102	22-23	-0.166523698239061
1102	24-25	0.002463117462774278
1102	26-27	-0.14526497443950603
1102	28-29	-0.47768732844586026
1102	30-31	-0.2415504384258753
1102	32-33	-0.25772349778210213
1102	34-35	-0.1811687636623276
1102	36-37	-0.22702959876885842
1102	38-39	-0.2427873074933089
1102	40-41	-0.7006657130277105
1102	42-43	-0.09217308770168842
1102	44-45	-0.2047940658642844
1102	46-47	-0.29287755120641634
1102	48-49	-0.5598833623655253
1102	50-51	-0.45519488457671997
1102	52-53	-0.607183329055097
1102	54-55	-0.2764027106638949
1102	56-57	-0.23168568220297203
1102	58-59	-0.21636622737889866
1102	60-61	-0.669045793757924
1102	62-63	-0.282192895051395
1102	64-65	-0.32848518665577586
1102	66-67	-0.3007391513385542
1102	68-69	-0.4850304176032125
1102	70-71	-0.20556696627438242
1102	72-73	-0.5045442180518762
1102	74-75	-0.33078303642545137
1102	76-77	-0.5389161663705266
1102	78-79	-0.29859330272043394
1102	80-81	-0.32553848036463506
1102	82-83	-0.3639400542648801
1102	84-85	-0.3411130305757055
1102	86-87	-0.439186014751467
1102	88-89	-0.32607161714711097
1102	90-91	-0.3551728294826333
1102	92-93	-0.357691881413988
1102	94-95	-0.5260605492634305
1102	96-97	-0.5076008211263527
1102	98-99	-0.610236761219177
1102	100	-0.7829583671344729
1103	1	-0.02251888293965365
1103	2	-0.052552786623984105
1103	3	-0.05927737965321711
1103	4	-0.0365203378208534
1103	5	0.0030287923436276287
1103	6	-0.047356454484031474
1103	7	0.1157050923365901
1103	8	-0.02754581606165374
1103	9	-0.09470988218689769
1103	10-11	-0.08872657930689343
1103	12-13	-0.045887516156369657
1103	14-15	-0.07234322785864578
1103	16-17	-0.06817735682504633
1103	18-19	-0.15257658791392004
1103	20-21	-0.15861414124205453
1103	22-23	-0.22550117164399097
1103	24-25	-0.14897635393115394
1103	26-27	-0.321067558322774
1103	28-29	-0.39345543802009075
1103	30-31	-0.26724186801125427
1103	32-33	-0.3448612429257025
1103	34-35	-0.41857643081625184
1103	36-37	-0.47177678716926863
1103	38-39	-0.30516265498251727
1103	40-41	-0.2588290266171711
1103	42-43	-0.24974442332767666
1103	44-45	-0.3462851219779779
1103	46-47	-0.3569549409604491
1103	48-49	-0.375331950862261
1103	50-51	-0.41334184821815256
1103	52-53	-0.22881405681789602
1103	54-55	-0.16462483336877654
1103	56-57	-0.12966509056300168
1103	58-59	-0.1008925436130852
1103	60-61	-0.0639430045497349
1103	62-63	-0.09912683619158713
1103	64-65	-0.15617346454574488
1103	66-67	-0.17824850925455138
1103	68-69	-0.135437053907701
1103	70-71	-0.10148412653767735
1103	72-73	-0.14411037408191874
1103	74-75	-0.12039722834933997
1103	76-77	-0.27897201813100025
1103	78-79	-0.27249042056679684
1103	80-81	-0.29204956199607324
1103	82-83	-0.2760417196781937
1103	84-85	-0.2985475674116742
1103	86-87	-0.39783805236902126
1103	88-89	-0.245852519393857
1103	90-91	-0.3010665421460246
1103	92-93	-0.309425102649481
1103	94-95	-0.46188666524244226
1103	96-97	-0.38567904738935255
1103	98-99	-0.30607276321327603
1103	100	-0.22492568413757397
1104	1	0.01097894791954701
1104	2	0.021058458814209757
1104	3	-0.020403071534332184
1104	4	-0.0702421450786872
1104	5	-0.05705880824223186
1104	6	-0.052866246058655975
1104	7	0.07555062585477401
1104	8	-0.104172810818838
1104	9	-0.10394095917312995
1104	10-11	-0.16455888924180329
1104	12-13	-0.14556725912971302
1104	14-15	-0.11315927358629096
1104	16-17	-0.12197853689330174
1104	18-19	-0.22529112759337266
1104	20-21	-0.2289831908905029
1104	22-23	-0.14118917370354467
1104	24-25	-0.1812998916310633
1104	26-27	-0.1743399168241737
1104	28-29	-0.28393553654465364
1104	30-31	-0.2512972219795486
1104	32-33	-0.1772352431553159
1104	34-35	-0.2872804779076574
1104	36-37	-0.362647209273284
1104	38-39	-0.4855496213512751
1104	40-41	-0.26093631124253847
1104	42-43	-0.2335112137298765
1104	44-45	-0.25042431923932895
1104	46-47	-0.31279716597358487
1104	48-49	-0.27678259638603464
1104	50-51	-0.41155951723544604
1104	52-53	-0.31686369135159254
1104	54-55	-0.2553966787213895
1104	56-57	-0.8613072884401234
1104	58-59	-0.22012346404545013
1104	60-61	-0.22995599504169917
1104	62-63	-0.2513981370846352
1104	64-65	-0.30414602239240196
1104	66-67	-0.34783237168196735
1104	68-69	-0.34723051760981605
1104	70-71	-0.3257642396739371
1104	72-73	-0.26721134088901977
1104	74-75	-0.2707204251097224
1104	76-77	-0.3507057652304013
1104	78-79	-0.2668877055748595
1104	80-81	-0.32879647701516035
1104	82-83	-0.19444243495541258
1104	84-85	-0.3669648078192722
1104	86-87	-0.38123502774831763
1104	88-89	-0.33562572502562205
1104	90-91	-0.6037703143984174
1104	92-93	-0.7677041059927774
1104	94-95	-0.38218538741498875
1104	96-97	-0.4427395571983226
1104	98-99	-0.5575066052886974
1104	100	-0.48864202940495005
1105	1	-0.05702633800273915
1105	2	-0.057246742529507344
1105	3	-0.02228002436234533
1105	4	-0.008920698447425934
1105	5	-0.02627239083376054
1105	6	-0.028684191020957428
1105	7	0.08524935564883407
1105	8	-0.04574864794008704
1105	9	-0.06874684298246336
1105	10-11	-0.10947886450355071
1105	12-13	-0.009852463047934634
1105	14-15	-0.09387437389354858
1105	16-17	-0.07738007900718458
1105	18-19	-0.11168928200351758
1105	20-21	-0.07363173549946112
1105	22-23	-0.13141598732850923
1105	24-25	-0.13430548566783074
1105	26-27	-0.2321865462373225
1105	28-29	-0.1769767576665231
1105	30-31	-0.09652828503886468
1105	32-33	-0.15673147049409408
1105	34-35	-0.2918747070418064
1105	36-37	-0.16351051140991757
1105	38-39	-0.11124589733077528
1105	40-41	-0.1820997759285703
1105	42-43	-0.22131986781192126
1105	44-45	-0.30376936599053295
1105	46-47	-0.13043192787744573
1105	48-49	-0.16450659764876008
1105	50-51	-0.2990687575764426
1105	52-53	-0.21430916419171098
1105	54-55	-0.2205977109597086
1105	56-57	-0.12560433081331013
1105	58-59	-0.14890694639388613
1105	60-61	-0.13019118556233877
1105	62-63	-0.16991657238913405
1105	64-65	-0.10860421447418389
1105	66-67	-0.1608792063916482
1105	68-69	-0.11803240826958472
1105	70-71	-0.1787955046145271
1105	72-73	-0.1424817011842734
1105	74-75	-0.11866599798540278
1105	76-77	-0.15262918978341844
1105	78-79	-0.18631189670185933
1105	80-81	-0.08429862743506078
1105	82-83	-0.15074006343552782
1105	84-85	-0.17144552071876262
1105	86-87	-0.13449168117389831
1105	88-89	-0.03889288934461277
1105	90-91	-0.18920378763776569
1105	92-93	-0.1569409529071848
1105	94-95	-0.14861566740037446
1105	96-97	-0.12380025836789699
1105	98-99	-0.1872134075761771
1105	100	-0.35330704673696545
1106	1	0.028295922389940387
1106	2	0.015230794610552323
1106	3	-0.00729003643079551
1106	4	0.033881673245403476
1106	5	-0.03105561313154226
1106	6	-0.07223066175055948
1106	7	0.018294003166289485
1106	8	-0.009880310835171713
1106	9	-0.045312123743990185
1106	10-11	-0.035226656811126134
1106	12-13	-0.02186780986369996
1106	14-15	-0.020293375790629398
1106	16-17	-0.1171499877229536
1106	18-19	-0.10301708151477129
1106	20-21	-0.06385966116064878
1106	22-23	-0.11228922790532181
1106	24-25	-0.07326712284049819
1106	26-27	-0.14263253719445146
1106	28-29	-0.14605813112356003
1106	30-31	-0.17343897137494224
1106	32-33	-0.15946277009598475
1106	34-35	-0.19219105957099458
1106	36-37	-0.15472140871275286
1106	38-39	-0.16376269272370791
1106	40-41	-0.08422584826983126
1106	42-43	-0.04208559020438685
1106	44-45	-0.1947185256267474
1106	46-47	-0.22642370001664602
1106	48-49	-0.2010392611959375
1106	50-51	-0.16627278858033634
1106	52-53	-0.12943122636345095
1106	54-55	-0.04522417694165881
1106	56-57	-0.0816610603430874
1106	58-59	-0.007813300593525696
1106	60-61	0.02654496881576307
1106	62-63	-0.05321002855708201
1106	64-65	-0.007464550211508936
1106	66-67	-0.036324753197426674
1106	68-69	-0.03138931130261824
1106	70-71	-0.024866646975205242
1106	72-73	0.02052353637711235
1106	74-75	-0.020980868891463444
1106	76-77	-0.046586909726173076
1106	78-79	-0.04180969755343966
1106	80-81	-0.05436232188797163
1106	82-83	-0.015693253924958128
1106	84-85	-0.02159786428656929
1106	86-87	-0.06641119951248342
1106	88-89	-0.0739110721540186
1106	90-91	-0.03672253120107882
1106	92-93	-0.08407532269410112
1106	94-95	-0.05633476773672896
1106	96-97	-0.02410881170910173
1106	98-99	-0.03457127041443542
1106	100	-0.09297749739955918
1107	1	-0.02623116730520536
1107	2	-0.020291697629232885
1107	3	0.01427668771994206
1107	4	0.06208455812073055
1107	5	0.04791542999408449
1107	6	0.07874595232188852
1107	7	0.2036846504668972
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2316	84-85	-1.4022201448342528
2316	86-87	0.21420630951908848
2316	88-89	0.3943112231136361
2316	90-91	-0.48932593899917265
2316	92-93	0.37422249974811805
2316	94-95	0.3827992738184989
2316	96-97	0.45274783822503295
2316	98-99	0.436637183090415
2316	100	0.4397243533249622
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	17892.0
1	1462.0
2	1009.0
3	839.0
4	727.0
5	768.0
6	838.0
7	1109.0
8	1846.0
9	2527.0
10	3954.0
11	7328.0
12	11875.0
13	15625.0
14	18227.0
15	20277.0
16	22733.0
17	26918.0
18	31838.0
19	37792.0
20	45342.0
21	54408.0
22	64001.0
23	71220.0
24	74718.0
25	76699.0
26	81969.0
27	89977.0
28	103573.0
29	122608.0
30	148815.0
31	186928.0
32	244798.0
33	340704.0
34	538504.0
35	976870.0
36	1215166.0
37	1306207.0
38	1389772.0
39	333528.0
40	335.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.81308390273186	17.97461573415933	13.031817878627441	23.18048248448137
2	15.826877482608309	20.95776304788659	38.810543992499795	24.4048154770053
3	20.823351507889633	24.973594216644088	29.558948382989954	24.64410589247633
4	13.158607615616644	15.89510611056335	37.9946228336967	32.951663440123305
5	13.499619451111736	38.14099519015438	33.84131476769813	14.51807059103575
6	31.106957336418713	35.92506356292495	17.670506176845254	15.297472923811084
7	27.566063306511047	32.773726245710236	21.722478676738177	17.93773177104054
8	26.190083056378743	32.98801067700039	20.18723095234555	20.63467531427531
9	26.720353883257502	16.70403170673772	20.670021889430526	35.90559252057425
10-11	25.657338067150242	25.096625795972454	27.871393554681067	21.37464258219623
12-13	26.984955231643294	23.077915244611898	27.374150312833027	22.56297921091178
14-15	23.692873388392204	24.758366818472048	26.49566898589924	25.053090807236504
16-17	22.73759219807941	27.631213158908373	26.691127208639987	22.94006743437223
18-19	22.430783663518422	27.912595828711762	27.759355968715045	21.897264539054774
20-21	23.742019739741963	25.95230165584307	27.352546845644742	22.953131758770223
22-23	23.110356120678254	25.755980142398194	27.73039416551879	23.40326957140476
24-25	23.818240033805374	25.767572403755107	27.431137201301087	22.983050361138428
26-27	23.19776256525938	25.269598810471127	28.740392522618603	22.792246101650885
28-29	22.4851273311765	27.516486870669173	27.568317424477367	22.430068373676963
30-31	23.562955157231393	25.78374423554452	27.458814139348537	23.19448646787555
32-33	23.49205788209711	26.022679767147107	27.69438712833912	22.790875222416666
34-35	24.117515221573328	25.93719838800749	27.52325727812296	22.42202911229622
36-37	23.572874673467144	25.64903541397871	28.406728337704497	22.37136157484965
38-39	23.216015313551157	26.56472633060118	27.921377828286698	22.297880527560967
40-41	23.253686609954993	26.555236537805776	27.453221741850832	22.7378551103884
42-43	22.703037309109657	26.78255566158808	26.639023719323546	23.875383309978716
44-45	23.072319056949045	25.91126842189825	27.25378740456502	23.762625116587692
46-47	23.74371815058493	26.362209092970918	27.074511768918452	22.819560987525705
48-49	23.367773241622288	26.396172305727138	27.164994568092947	23.071059884557627
50-51	22.2402768389051	26.65915351130772	28.218589776826025	22.881979872961153
52-53	23.153339590899233	27.26012816267375	26.51955384964254	23.066978396784478
54-55	22.394770475355998	26.078119423232	27.446869292349707	24.080240809062293
56-57	23.34455349271988	25.91950765434341	26.999293047754897	23.736645805181812
58-59	24.255595229949094	26.03944261952671	26.803340128911373	22.901622021612823
60-61	23.509279068071717	26.323816016010763	28.040788950034965	22.12611596588255
62-63	22.87139867493679	26.475235304401828	27.69375592012045	22.959610100540935
64-65	22.52441207432736	27.200328716782103	27.059447300226214	23.215811908664328
66-67	23.093966779975943	26.15938296165725	26.577199776833904	24.169450481532902
68-69	22.392888050008093	26.431894267622035	27.187904565799936	23.98731311656994
70-71	23.42460672540898	26.15034713987602	26.91198993697233	23.513056197742667
72-73	22.99310408092548	25.898305142249654	27.731684136071692	23.37690664075317
74-75	22.248694203558113	27.602922802483416	26.87036290911824	23.278020084840236
76-77	22.6388507674839	26.030399834689355	26.762954813002764	24.567794584823982
78-79	22.71574505576399	26.160876432049356	27.312380422683898	23.810998089502753
80-81	23.39638275127639	26.13017410982101	26.80346529857161	23.66997784033099
82-83	23.06894495564587	25.80342544571893	27.67561687638253	23.452012722252675
84-85	22.655143611193694	26.617387832980675	27.207462950353406	23.520005605472225
86-87	22.408597688911158	27.022117923986684	26.88945735341705	23.67982703368511
88-89	22.051997333817965	26.630123864742792	26.564362998935536	24.7535158025037
90-91	22.92189565663066	25.584824851344024	26.889658460512283	24.603621031513033
92-93	23.443060109403678	26.219304657451108	26.889754745137644	23.447880488007563
94-95	22.948419853492325	25.935607526810518	27.04064240460433	24.07533021509283
96-97	21.719968753963826	25.968876369363013	28.091405356508087	24.219749520165074
98-99	22.328577894074186	26.4081209324338	26.71900005095416	24.54430112253785
100	22.204463795126394	25.048870477898326	26.938576811287785	25.808088915687495
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11255.0
1	8667.5
2	4575.0
3	2515.0
4	1710.0
5	1614.5
6	2183.5
7	3536.5
8	5838.5
9	7369.5
10	6786.5
11	6083.0
12	6408.5
13	6986.5
14	7573.5
15	8011.0
16	8415.0
17	8970.0
18	9490.5
19	9995.5
20	10625.5
21	11629.5
22	13105.5
23	15351.5
24	18342.0
25	22534.5
26	28108.0
27	34679.0
28	42152.5
29	51648.0
30	63499.0
31	75097.5
32	88118.5
33	101254.0
34	113476.5
35	126543.0
36	143765.5
37	161073.0
38	174025.0
39	186224.5
40	195467.5
41	204181.0
42	214202.5
43	226634.5
44	240237.5
45	254239.5
46	273040.5
47	335695.5
48	452087.0
49	451494.5
50	353054.0
51	312259.0
52	298638.5
53	288328.5
54	277989.0
55	261363.5
56	238971.0
57	215856.0
58	191856.5
59	163956.5
60	136397.0
61	113620.5
62	93483.0
63	73177.5
64	54660.0
65	40921.0
66	30820.5
67	23649.5
68	18256.5
69	13728.0
70	10380.0
71	7707.5
72	5712.5
73	4360.5
74	3285.5
75	2450.0
76	1757.0
77	1183.5
78	795.0
79	543.0
80	371.0
81	269.5
82	200.0
83	156.0
84	130.0
85	104.5
86	81.0
87	63.5
88	53.5
89	50.0
90	40.0
91	32.5
92	28.0
93	20.5
94	16.5
95	15.5
96	15.5
97	13.0
98	9.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20528552369130154
2	0.18285882778455706
3	0.37224414910255516
4	0.3667577342198617
5	0.3784586190407719
6	0.3085653337105352
7	0.3278327907156339
8	0.3172759924105461
9	0.3311220394486231
10-11	0.3036314606110514
12-13	0.3117310731037481
14-15	0.29935413715985204
16-17	0.2943422581615622
18-19	0.31637242408270916
20-21	0.3198826895289822
22-23	0.3111395283711354
24-25	0.3160018960633803
26-27	0.30526308399441165
28-29	0.3302054701376518
30-31	0.31262164044845075
32-33	0.31158806228927033
34-35	0.3129661665015108
36-37	0.32255439156309
38-39	0.3291003865712325
40-41	0.35624643935574407
42-43	0.32231387337510464
44-45	0.3078372786550119
46-47	0.3146107908680054
48-49	0.33685547301086904
50-51	0.32728024893242424
52-53	0.35627894181357994
54-55	0.3561814344400724
56-57	0.3619733724264229
58-59	0.34702874231349373
60-61	0.33002995686533815
62-63	0.3534447274902928
64-65	0.34802331752327115
66-67	0.33395625377191024
68-69	0.3443505397878187
70-71	0.36439805578097817
72-73	0.367674303530833
74-75	0.3567209752401477
76-77	0.34007971682818655
78-79	0.3506040126754385
80-81	0.36439805578097817
82-83	0.370014480495015
84-85	0.36125181786246674
86-87	0.3615053370335865
88-89	0.34885538044386916
90-91	0.36041325445030153
92-93	0.34261490853938376
94-95	0.3626689250241103
96-97	0.3727901903941976
98-99	0.3637090036748579
100	0.3852971361694371
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7691726.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.905292429947494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8857372336168	36.59364475419628
2	15.06301532830025	15.335744003278117
3	5.529432183104049	8.444320867574238
4	2.5414367980787724	5.174903335937174
5	1.3950456871121297	3.550760432779
6	0.8292721957574927	2.5328606177435895
7	0.5503574563093468	1.9611275078100547
8	0.3835561308456261	1.5620029603196643
9	0.29431660688763733	1.348404564654457
>10	1.4185845243498374	12.652049748955562
>50	0.07252102294081876	2.514799757060149
>100	0.03370446099587549	3.0057630020934445
>500	0.0017847715647894948	0.5882110211938587
>1k	8.923778764171793E-4	0.7964882923596194
>5k	0.0	0.0
>10k+	3.432222600996003E-4	3.938919134044836
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	77973	1.0137256579342528	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	23548	0.3061471508475471	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23488	0.3053670918594864	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18229	0.23699492155596805	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	17948	0.23334164529521723	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	16518	0.21475023941310442	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	13329	0.17329010419767943	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	13017	0.1692337974597639	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	11252	0.14628706222764565	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10142	0.13185597094852314	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.600196626868924E-5
2	0.0	0.0	0.0	0.0	3.9002949403033855E-5
3	0.0	0.0	0.0	0.0	3.9002949403033855E-5
4	0.0	0.0	0.0	0.0	3.9002949403033855E-5
5	0.0	0.0	0.0	0.0	3.9002949403033855E-5
6	0.0	0.0	0.0	0.0	9.100688194041233E-5
7	0.0	0.0	0.0	0.0	9.100688194041233E-5
8	0.0	0.0	0.0	0.0	9.100688194041233E-5
9	0.0	0.0	0.0	1.300098313434462E-5	1.1700884820910158E-4
10-11	0.0	0.0	0.0	3.250245783586154E-5	1.1700884820910158E-4
12-13	0.0	0.0	0.0	4.5503440970206166E-5	1.4301081447779082E-4
14-15	0.0	0.0	0.0	7.150540723889541E-5	2.4701867955254775E-4
16-17	0.0	0.0	0.0	1.1700884820910158E-4	3.3152506992578776E-4
18-19	1.300098313434462E-5	0.0	0.0	2.080157301495139E-4	3.5102654462730473E-4
20-21	1.300098313434462E-5	0.0	0.0	2.9902261208992624E-4	3.965299855975109E-4
22-23	1.300098313434462E-5	0.0	0.0	4.940373591050956E-4	4.940373591050955E-4
24-25	1.300098313434462E-5	0.0	0.0	7.54057021791988E-4	5.785437494783356E-4
26-27	1.300098313434462E-5	0.0	0.0	0.0014171071616435635	5.980452241798525E-4
28-29	1.300098313434462E-5	0.0	0.0	0.0036207738029149766	5.980452241798525E-4
30-31	1.300098313434462E-5	0.0	0.0	0.010166768811057492	6.110462073141971E-4
32-33	1.300098313434462E-5	0.0	0.0	0.028231634876229345	6.760511229859202E-4
34-35	1.300098313434462E-5	0.0	0.0	0.05659327958380213	7.15054072388954E-4
36-37	1.300098313434462E-5	0.0	0.0	0.09848244724266049	7.215545639561263E-4
38-39	1.300098313434462E-5	0.0	0.0	0.15969107583915496	7.475565302248156E-4
40-41	1.300098313434462E-5	0.0	0.0	0.22570356770378974	7.735584964935049E-4
42-43	1.300098313434462E-5	0.0	0.0	0.29416674488924854	7.995604627621941E-4
44-45	1.300098313434462E-5	0.0	0.0	0.378426116582936	8.19061937463711E-4
46-47	1.300098313434462E-5	0.0	0.0	0.4800092983031377	8.84066853135434E-4
48-49	1.300098313434462E-5	0.0	0.0	0.5880214661832728	9.88074718210191E-4
50-51	1.300098313434462E-5	0.0	0.0	0.705920881737077	9.945752097773634E-4
52-53	1.300098313434462E-5	0.0	0.0	0.8274150691275275	0.0010595801254490865
54-55	1.300098313434462E-5	0.0	0.0	0.9682287174556139	0.0011310855326879818
56-57	1.300098313434462E-5	0.0	0.0	1.138405086192618	0.0011570874989566711
58-59	1.300098313434462E-5	0.0	0.0	1.3263537988742709	0.001176588973658188
60-61	1.300098313434462E-5	0.0	0.0	1.5249958201839222	0.0012220924146283943
62-63	1.300098313434462E-5	0.0	0.0	1.7378349150762782	0.0012870973303001174
64-65	1.300098313434462E-5	0.0	0.0	1.952377138759233	0.001300098313434462
66-67	2.600196626868924E-5	0.0	0.0	2.209036047305897	0.0013456017544046682
68-69	2.600196626868924E-5	0.0	0.0	2.50360582267231	0.001365103229106185
70-71	2.600196626868924E-5	0.0	0.0	2.8201667610104675	0.001365103229106185
72-73	2.600196626868924E-5	0.0	0.0	3.1283550662101067	0.0015796194508228712
74-75	2.600196626868924E-5	0.0	0.0	3.4546797428821567	0.0015861199423900436
76-77	2.600196626868924E-5	0.0	0.0	3.8068828764831197	0.0015991209255243881
78-79	2.600196626868924E-5	0.0	0.0	4.234842738807909	0.0016251228917930774
80-81	2.600196626868924E-5	0.0	0.0	4.678585794657792	0.0016836273158976282
82-83	3.9002949403033855E-5	0.0	0.0	5.1167969321840125	0.001846139605076936
84-85	3.9002949403033855E-5	0.0	0.0	5.572058859090925	0.001852640096644108
86-87	3.9002949403033855E-5	0.0	0.0	6.040484281421361	0.0018591405882112805
88	3.9002949403033855E-5	0.0	0.0	6.415751159102651	0.0018591405882112805
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	31215	0.0	28.393675	1
TACATGG	31670	0.0	27.627625	2
ACATGGG	32780	0.0	25.846708	3
CATGGGG	23850	0.0	24.660393	4
ATGGGGG	15625	0.0	22.777992	5
GAGTACT	19715	0.0	20.626678	12-13
GTATAGG	2015	0.0	19.834328	1
TGGGGGG	14650	0.0	19.082138	6
GTACTTT	21290	0.0	18.912115	14-15
AGAGTAC	34850	0.0	18.766354	10-11
TACCTGG	5270	0.0	18.378153	2
AGTACTT	20885	0.0	17.873863	12-13
GAGTACA	24115	0.0	17.041105	1
AGTACAT	23450	0.0	16.079657	2
ACTTTTT	25770	0.0	15.805767	16-17
CATGGGA	10295	0.0	15.66386	4
TACTTTT	24365	0.0	15.175508	14-15
TACCGTA	410	1.2376404E-8	14.896898	7
ATGGGGA	8220	0.0	14.871032	5
GTACCTG	6695	0.0	14.818542	1
>>END_MODULE