FastQCFastQC Report
Mon 6 Jun 2016
ERR522922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7691726
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT808231.050778459867135No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT234370.30470404171963483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216750.28179630943691963No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC188200.2446785025883657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182740.2375799657970136No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC169430.22027565724520087No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA138030.17945257020335878No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC102180.13284404566673333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98190.1276566533961298No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG310700.026.8963721
TACATGG316100.026.0804672
ACATGGG323850.025.0030163
CATGGGG234700.023.407364
ATGGGGG148150.022.712075
GAGTACT202450.021.43140612-13
GTACTTT218000.019.74938214-15
TGGGGGG127550.019.269336
AGAGTAC362100.018.12922310-11
AGTACTT216400.017.63865912-13
GAGTACA246350.016.7224181
ACTTTTT263650.016.53309216-17
AGTACAT237050.016.2586382
CATGGGA104700.015.8894034
TACCTGG50600.015.8838532
GGGTTAG26350.015.3484791
TACTTTT250850.014.99898214-15
GTATAGG22950.014.7535771
GTAAGTA50050.014.6497712
TATAGGG11400.014.4302382
Produced by FastQC (version 0.11.5)