##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522921_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3365515 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03030086034381 33.0 31.0 34.0 30.0 34.0 2 32.06101829883391 33.0 31.0 34.0 30.0 34.0 3 32.27151119516627 34.0 31.0 34.0 30.0 35.0 4 35.29151794004781 37.0 35.0 37.0 32.0 37.0 5 35.25908040819904 36.0 35.0 37.0 32.0 37.0 6 35.50847611732528 36.0 35.0 37.0 33.0 37.0 7 35.35162374851991 36.0 35.0 37.0 33.0 37.0 8 35.42170514765199 36.0 35.0 37.0 33.0 37.0 9 36.54358723702019 37.0 35.0 39.0 33.0 39.0 10-11 36.34709784386639 37.0 35.0 39.0 32.0 39.0 12-13 36.44920955633833 37.0 35.0 39.0 33.0 39.0 14-15 37.27667771500053 38.0 36.5 40.0 32.0 41.0 16-17 37.14974914686162 38.0 36.5 40.0 32.5 41.0 18-19 37.19719359444245 38.0 36.0 40.0 32.0 41.0 20-21 37.262409616358866 38.0 36.0 40.0 32.5 41.0 22-23 37.19194179791206 38.0 35.5 40.0 32.0 41.0 24-25 37.036105024045355 38.0 36.5 40.0 31.5 41.0 26-27 36.576456797845196 37.5 35.5 40.0 31.0 41.0 28-29 36.65767779968296 38.0 36.0 40.0 31.0 41.0 30-31 36.49709658105817 37.0 35.5 40.0 31.0 41.0 32-33 36.300938192223185 37.5 35.0 40.0 30.0 41.0 34-35 36.02557305494108 37.0 35.0 40.0 30.0 41.0 36-37 35.83170569734499 37.0 35.0 40.0 30.0 41.0 38-39 35.721570695718185 36.5 34.5 40.0 30.0 41.0 40-41 36.03614023410979 37.5 35.0 40.0 30.0 41.0 42-43 36.04396934793041 37.5 35.0 40.0 30.0 41.0 44-45 35.848015979723755 37.0 35.0 40.0 30.0 41.0 46-47 35.65485341767902 37.0 35.0 40.0 28.0 41.0 48-49 35.38272077230379 37.5 34.5 40.0 28.0 41.0 50-51 35.15236702258049 37.5 34.0 40.0 27.0 41.0 52-53 35.33379542209737 37.5 35.0 40.0 26.5 41.0 54-55 35.25735006380896 38.0 34.0 40.0 26.0 41.0 56-57 34.87960133887384 37.5 34.5 40.0 25.0 41.0 58-59 34.748427655202846 37.0 34.0 40.0 25.0 41.0 60-61 34.29876378503735 37.0 33.5 39.0 22.5 41.0 62-63 34.06855756102706 36.5 33.5 39.0 23.5 41.0 64-65 33.92834009059535 37.0 33.0 39.0 23.0 40.5 66-67 33.18799069384626 36.0 33.0 38.0 22.5 39.5 68-69 32.94652334041001 36.5 32.5 37.0 22.0 39.0 70-71 32.26075281197677 35.0 31.5 37.0 21.0 38.5 72-73 31.877323232848465 35.0 31.5 36.0 20.5 37.0 74-75 31.730954252172403 35.0 31.0 37.0 20.0 38.0 76-77 30.12440087178337 33.5 28.5 35.5 17.5 36.0 78-79 31.105896274418626 34.0 30.5 36.0 19.0 37.0 80-81 31.165308429764835 34.0 31.0 36.0 19.5 37.5 82-83 31.114935604209165 34.0 30.5 36.0 19.0 37.0 84-85 30.797843123563556 34.0 31.0 35.0 18.0 36.0 86-87 30.82701711327984 34.0 30.0 35.0 18.0 36.0 88-89 30.711780515017765 34.0 30.5 35.0 17.5 37.0 90-91 30.42077497797514 34.0 30.0 35.0 16.0 36.0 92-93 30.22701443909773 34.0 29.0 35.0 15.0 36.5 94-95 30.361265066416284 34.0 29.5 35.0 15.0 36.5 96-97 30.095513910946764 34.0 29.0 35.0 12.5 37.0 98-99 30.00665173086437 34.0 29.0 35.0 10.0 36.0 100 28.697062113822106 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13839017983847413 1101 2 -0.12785161417797042 1101 3 -0.1141358175757361 1101 4 -0.0727030271163116 1101 5 -0.1708026518652659 1101 6 -0.09045971754211024 1101 7 -0.04165959034331479 1101 8 -0.14201996219355095 1101 9 -0.1817272339668321 1101 10-11 -0.1442774948305967 1101 12-13 -0.19937094765375463 1101 14-15 -0.3394122568171696 1101 16-17 -0.2811188546524477 1101 18-19 -0.41609467343567985 1101 20-21 -0.31382793688600685 1101 22-23 -0.7917218167510072 1101 24-25 -0.39970002212426436 1101 26-27 -0.2088617728327975 1101 28-29 -0.29731353074981826 1101 30-31 -0.27768503829720714 1101 32-33 -0.351725032845394 1101 34-35 -0.4704597370901453 1101 36-37 -0.41546194462844 1101 38-39 -0.3062821220932648 1101 40-41 -0.39594399927820945 1101 42-43 -0.4434596838186664 1101 44-45 -0.5303478668236892 1101 46-47 -0.4425528482446026 1101 48-49 -0.534399849906201 1101 50-51 -0.4610773336955063 1101 52-53 -0.4587981996617927 1101 54-55 -0.5665946187968345 1101 56-57 -0.6114512981846119 1101 58-59 -0.49772414348532834 1101 60-61 -0.6283622342888009 1101 62-63 -0.49051129403050453 1101 64-65 -0.49635435530899485 1101 66-67 -0.3450366339675739 1101 68-69 -0.30020323284055195 1101 70-71 -0.4292600866616141 1101 72-73 -0.4041181944140426 1101 74-75 -0.34334349327016156 1101 76-77 -0.3282082111231368 1101 78-79 -0.2618185908163966 1101 80-81 -0.23571975116045607 1101 82-83 -0.29359268850746645 1101 84-85 -0.384317416685537 1101 86-87 -0.5334604088714592 1101 88-89 -0.5735346508898935 1101 90-91 -0.6252754393111744 1101 92-93 -0.6377416914070473 1101 94-95 -0.7417882572923844 1101 96-97 -0.8915794014493947 1101 98-99 -0.9895471040505512 1101 100 -0.9293177851537315 1102 1 -0.05342355025606338 1102 2 -0.11408586800567377 1102 3 -0.1140352601115282 1102 4 -0.18552751803431278 1102 5 -0.13340816463798433 1102 6 -0.13883326943344798 1102 7 -0.10484568964155017 1102 8 -0.23754650301776792 1102 9 -0.23045214662597857 1102 10-11 -0.18540938420990472 1102 12-13 -0.1829026458361369 1102 14-15 -0.3305070668614647 1102 16-17 -0.3974548378028757 1102 18-19 -0.2767592697205856 1102 20-21 -0.3590430791871242 1102 22-23 -0.28645012612716414 1102 24-25 -0.193937164371647 1102 26-27 -0.24644237008313752 1102 28-29 -0.1596538059919297 1102 30-31 -0.33402191485445343 1102 32-33 -0.2389532312836451 1102 34-35 -0.13688416062873898 1102 36-37 -0.2228700976347895 1102 38-39 -0.12968402833612203 1102 40-41 -0.11376922666647715 1102 42-43 -0.24022807980805538 1102 44-45 -0.11502320445620029 1102 46-47 -0.03639888886332443 1102 48-49 -0.15875479886344834 1102 50-51 0.1148201834895346 1102 52-53 -0.06745014617191458 1102 54-55 0.0064759742157178835 1102 56-57 -0.09951698680695387 1102 58-59 -0.08762635381120276 1102 60-61 -0.22616115734000175 1102 62-63 -0.21497419959219144 1102 64-65 -0.08958696587817627 1102 66-67 -0.015831125504469412 1102 68-69 0.040800854102094775 1102 70-71 5.016561035375844E-4 1102 72-73 0.007847927363012985 1102 74-75 -0.12078891649360557 1102 76-77 -0.0699437306269104 1102 78-79 -0.0868345329134037 1102 80-81 -0.11449010260879433 1102 82-83 -0.13352001360119203 1102 84-85 -0.13476739165932017 1102 86-87 -0.18985333981947505 1102 88-89 -0.16523297298726192 1102 90-91 -0.2576656872837155 1102 92-93 -0.3833677614904829 1102 94-95 -0.5915745431630874 1102 96-97 -0.5431633340196136 1102 98-99 -0.5872789723934808 1102 100 -0.648637009153159 1103 1 -0.09341628472974506 1103 2 -0.08127285045224397 1103 3 -0.27026411466717803 1103 4 -0.010240644322628611 1103 5 -0.17482067263239287 1103 6 -0.1913653857084583 1103 7 -0.12128756379417638 1103 8 -0.11412243228146224 1103 9 -0.16836076428023716 1103 10-11 -0.2696759484740525 1103 12-13 -0.17730385546095562 1103 14-15 -0.2188144303692141 1103 16-17 -0.26465224633923157 1103 18-19 -0.3646229341627816 1103 20-21 -0.2527493914156693 1103 22-23 -0.33569544487733083 1103 24-25 -0.25096117939632734 1103 26-27 -0.1701332634045869 1103 28-29 -0.23301379744471973 1103 30-31 -0.35327565879782696 1103 32-33 -0.22471001213565955 1103 34-35 -0.29045034462333064 1103 36-37 -0.44272314829774473 1103 38-39 -0.3302670659907676 1103 40-41 -0.3164994832881618 1103 42-43 -0.358860067706253 1103 44-45 -0.22073165229713254 1103 46-47 -0.35359783998480054 1103 48-49 -0.37368214211225137 1103 50-51 -0.33571848624259104 1103 52-53 -0.4155034781508178 1103 54-55 -1.1579990642273899 1103 56-57 -0.4658773321119014 1103 58-59 -0.360429953163262 1103 60-61 -0.36323646158392364 1103 62-63 -0.32112230899291916 1103 64-65 -0.26404967256845424 1103 66-67 -0.20314178062102428 1103 68-69 -0.20373142565717473 1103 70-71 -0.31751213708747983 1103 72-73 -0.3528477576940787 1103 74-75 -0.49315105873661835 1103 76-77 -0.32531358847396774 1103 78-79 -0.41712541883442356 1103 80-81 -1.2449939942385662 1103 82-83 -0.26815133724537077 1103 84-85 -0.30824815115879645 1103 86-87 -0.3066237102016025 1103 88-89 -0.36365579626325584 1103 90-91 -0.33854144112919116 1103 92-93 -0.2145781471853745 1103 94-95 -0.4376452316550363 1103 96-97 -0.3241667901046945 1103 98-99 -0.34178518097618493 1103 100 -0.4304606145942067 1104 1 -0.07158257861598827 1104 2 -0.20380445814253179 1104 3 -0.11579204724582581 1104 4 -0.025993664709154984 1104 5 -0.05245038684979875 1104 6 -0.21305054983407956 1104 7 -0.35834170189281167 1104 8 -0.13409656179198493 1104 9 -0.2668127424544977 1104 10-11 -0.28826724153451977 1104 12-13 -0.20062730696725595 1104 14-15 -0.40980757561359127 1104 16-17 -0.31359786365260334 1104 18-19 -0.27743984667578303 1104 20-21 -0.34123499847364513 1104 22-23 -0.28886480594427155 1104 24-25 -0.22964220911966748 1104 26-27 -0.2759835370128414 1104 28-29 -0.31153642498045286 1104 30-31 -0.35313095078556245 1104 32-33 -0.2317624632849089 1104 34-35 -0.2050438134159478 1104 36-37 -0.30061988025603625 1104 38-39 -0.22156342957279662 1104 40-41 -0.15540559885324257 1104 42-43 -0.18450077422739497 1104 44-45 -0.22659660315146368 1104 46-47 -0.14624408748584017 1104 48-49 -0.18837699498981664 1104 50-51 -0.12485305761588705 1104 52-53 -0.25024855021383274 1104 54-55 -0.15477115146745035 1104 56-57 -0.17355259789490418 1104 58-59 -0.09240771059802455 1104 60-61 -0.20089029249260193 1104 62-63 -0.2121673013878862 1104 64-65 -0.37126528396768066 1104 66-67 -0.2835168574971547 1104 68-69 -0.1530578157585083 1104 70-71 -0.09228375009421796 1104 72-73 -0.023874539686740803 1104 74-75 -0.1721834772636477 1104 76-77 -0.1354066380852217 1104 78-79 -0.0022108791396000527 1104 80-81 -0.08291969335915184 1104 82-83 -0.18563149494564612 1104 84-85 -0.841100703918233 1104 86-87 -0.16927270440227105 1104 88-89 -0.3330108763009427 1104 90-91 -0.3669501209359751 1104 92-93 -0.23664130200879185 1104 94-95 -0.2701215730747144 1104 96-97 -0.05402563687621509 1104 98-99 -0.08559350656291898 1104 100 -1.1275920535109094 1105 1 -0.06750233518863524 1105 2 -0.15938748669825742 1105 3 -0.18587856157682836 1105 4 -0.17671501599362927 1105 5 -0.1338036146078636 1105 6 -0.17641442165593446 1105 7 -0.16083556459030746 1105 8 -0.22304047891676504 1105 9 -0.33992160309483666 1105 10-11 -0.2981247380768295 1105 12-13 -0.1999254265311734 1105 14-15 -0.35657947595321815 1105 16-17 -0.27560703127193875 1105 18-19 -0.4010580521847089 1105 20-21 -0.3408560190952912 1105 22-23 -0.3494693397294171 1105 24-25 -0.1844769574105598 1105 26-27 -0.32578212949443497 1105 28-29 -0.27770653756977737 1105 30-31 -0.34893693763496003 1105 32-33 -0.25171410498487035 1105 34-35 -0.3466074136327464 1105 36-37 -0.5594257896774977 1105 38-39 -0.5000561505878025 1105 40-41 -0.35896017681785963 1105 42-43 -0.43331667716616806 1105 44-45 -0.6376553903111741 1105 46-47 -0.6026558131875106 1105 48-49 -0.5150083996843833 1105 50-51 -0.5973168942156164 1105 52-53 -0.6176187198872825 1105 54-55 -1.536549796387483 1105 56-57 -0.5360849923765727 1105 58-59 -0.5566783242675513 1105 60-61 -0.44674875194997554 1105 62-63 -0.3527376226530876 1105 64-65 -0.38548388997638483 1105 66-67 -0.496953033346621 1105 68-69 -0.33310561422423035 1105 70-71 -0.06341622473656372 1105 72-73 -0.18522448814402637 1105 74-75 -0.17974361295989993 1105 76-77 -0.25997860203720435 1105 78-79 -0.25729142316952647 1105 80-81 -0.23016319244899464 1105 82-83 -0.21735559871729748 1105 84-85 -0.21034759958940796 1105 86-87 -0.2204705031716223 1105 88-89 -0.3292037656078399 1105 90-91 -0.30171129750658565 1105 92-93 -0.1360167735980511 1105 94-95 -0.3159929276992095 1105 96-97 -0.2642337510201713 1105 98-99 -0.3211783417704659 1105 100 -0.1091480741401405 1106 1 -0.025258409868314402 1106 2 -0.1703381964002446 1106 3 -0.07664076369935202 1106 4 -0.12433574123377866 1106 5 -0.059693473533883434 1106 6 -0.1707896280784098 1106 7 -0.13391247611593826 1106 8 -0.18337605531749546 1106 9 -0.16726963633285408 1106 10-11 -0.24995564005785553 1106 12-13 -0.2128595212314579 1106 14-15 -0.2239123796577971 1106 16-17 -0.14376489681367133 1106 18-19 -0.2625242572355688 1106 20-21 -0.26841964636452786 1106 22-23 -0.25896667135624796 1106 24-25 -0.16836410441503347 1106 26-27 -0.023330069337795578 1106 28-29 -0.06041120742077055 1106 30-31 -0.220201799939737 1106 32-33 -0.07469132850252436 1106 34-35 -0.17539254322351638 1106 36-37 -0.0891266455645976 1106 38-39 -0.08143459269545161 1106 40-41 -0.1110832852052539 1106 42-43 -0.08432696759695801 1106 44-45 -0.12442938043711393 1106 46-47 -0.15321160784704801 1106 48-49 -0.10980210734928875 1106 50-51 -0.03223801250145897 1106 52-53 -0.10718836092763695 1106 54-55 -0.06535106208436048 1106 56-57 0.08096106997214036 1106 58-59 -0.07317769752589953 1106 60-61 -0.04226508049711697 1106 62-63 -0.13760674226877967 1106 64-65 0.039986548504323594 1106 66-67 -0.09603345731940038 1106 68-69 0.021311214797030686 1106 70-71 0.03603806908309082 1106 72-73 -0.04541090794980818 1106 74-75 -0.09895869378468092 1106 76-77 -0.03810673364084849 1106 78-79 0.003978072463851845 1106 80-81 0.03868258268976987 1106 82-83 0.07758762048228363 1106 84-85 0.06665000347530636 1106 86-87 0.1572017493059228 1106 88-89 0.06332467120756391 1106 90-91 0.05398882065194144 1106 92-93 0.16480629522721912 1106 94-95 0.15115812855136213 1106 96-97 0.2111749780034593 1106 98-99 -0.04034934636183962 1106 100 0.08099241009411884 1107 1 -0.02999866805413376 1107 2 -0.12421992006046167 1107 3 -0.09121179521689271 1107 4 -0.07233239201408992 1107 5 -0.23809728813095887 1107 6 -0.22476663747073644 1107 7 -0.18140577200693286 1107 8 -0.18360329036997314 1107 9 -0.1254829550607468 1107 10-11 -0.15009879364121304 1107 12-13 -0.14785612215505495 1107 14-15 -0.24603643433747635 1107 16-17 -0.12574056745405215 1107 18-19 -0.251224645524033 1107 20-21 -0.1198656082491496 1107 22-23 -0.08775527364198865 1107 24-25 -0.11126889013823416 1107 26-27 -0.13332308399728987 1107 28-29 -0.09456652568393764 1107 30-31 -0.1902977120306346 1107 32-33 0.003590832096961094 1107 34-35 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2316 100 -0.14008777887521973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 0.0 11 4.0 12 19.0 13 226.0 14 925.0 15 2695.0 16 5920.0 17 11306.0 18 16679.0 19 22629.0 20 29568.0 21 37228.0 22 39410.0 23 37818.0 24 39461.0 25 42611.0 26 48083.0 27 55858.0 28 66555.0 29 79240.0 30 95542.0 31 116071.0 32 143540.0 33 184111.0 34 253024.0 35 443725.0 36 669608.0 37 539050.0 38 369626.0 39 14974.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.96343369853461 17.181274767165238 13.21912284513958 17.636168689160574 2 12.99071283238554 27.077711421763052 30.278995394304197 29.652580351547215 3 31.84294231343494 25.099279010790326 30.38768212294404 12.670096552830696 4 13.076777710824041 17.52190159702488 37.35510359803037 32.04621709412071 5 9.460980057328829 42.765362738400896 34.30821658732722 13.465440616943056 6 27.031235050574743 32.408600885332824 15.297187888685057 25.26297617540738 7 19.864121835736878 35.94828726064213 22.51833671815458 21.669254185466414 8 25.174839511932053 22.936549086841094 21.537209015559284 30.35140238566757 9 20.935369475399753 22.83285024728756 25.877525430729026 30.354254846583657 10-11 25.668289350707003 23.41622655317634 23.925747460009458 26.989736636107196 12-13 34.62991837040922 22.425934275885584 22.96669967308051 19.977447680624692 14-15 27.97090684541405 24.1198869045293 30.20931042162965 17.699895828427 16-17 19.353740263583738 27.994688984381916 32.158981036162096 20.492589715872253 18-19 19.774242541519282 32.99177584883381 28.716530563157576 18.517451046489324 20-21 22.571049173878603 25.256159140165792 25.011781359363848 27.16101032659176 22-23 18.536622181376316 27.35112180394293 25.96734298629815 28.144913028382607 24-25 26.82157588068351 27.7949461114071 22.203122451059603 23.180355556849783 26-27 25.147530397242544 20.812101809087086 32.14228772821985 21.89808006545052 28-29 19.744938820085732 30.785183093987506 28.446661664301686 21.023216421625072 30-31 23.136193809753188 26.032710715004203 26.416596116066906 24.414499359175704 32-33 20.463163568796432 27.30119048432681 30.194451023083147 22.04119492379361 34-35 22.55295635595134 25.26283540115012 28.649979912184154 23.534228330714388 36-37 22.732447772729454 23.898503088893666 32.3544247151905 21.014624423186383 38-39 23.777502325521276 24.853147844318663 29.912480765513617 21.456869064646444 40-41 20.05388459206431 28.48022362075992 28.786329347654423 22.67956243952135 42-43 21.031342283913382 28.066626571110874 24.6860304032872 26.216000741688543 44-45 24.04899556768463 22.94198647127553 26.245140083273803 26.763877877766035 46-47 24.78757457828065 27.82378151351655 28.991996890345913 18.396647017856885 48-49 23.435044518146046 25.476831544286345 29.862344424235943 21.22577951333167 50-51 15.836968727168118 27.803062126192383 34.48017570546006 21.87979344117943 52-53 19.03777580096819 30.49780684582663 24.9963114357675 25.468105917437672 54-55 16.975343125165285 25.81810726245695 27.09963778343669 30.106911828941072 56-57 23.4771189354192 22.71034096882344 25.53422628819738 28.278313807559975 58-59 26.843463436507953 22.23821103107957 26.79487792853978 24.123447603872698 60-61 22.274802444078997 25.733162043174453 30.717889892241413 21.274145620505138 62-63 17.696963862381015 26.83543160404093 30.512839140700688 24.954765392877366 64-65 15.994733043511076 32.73595005335 27.184846521078075 24.08447038206085 66-67 20.959373071881522 26.480932417111987 23.419758106510212 29.139936404496286 68-69 19.711639672008253 26.467269536889116 25.386230295157215 28.434860495945415 70-71 23.956766117215274 27.0487802957562 22.625793397936746 26.36866018909178 72-73 22.815175103635312 22.751334385410146 29.6617169929224 24.77177351803214 74-75 20.111947899089998 30.62053171537706 24.141718659067777 25.12580172646517 76-77 20.374121870413234 25.786934706516067 26.15900922363858 27.67993419943212 78-79 21.460990747722217 26.476653877153822 27.72668823426723 24.33566714085674 80-81 22.45694938218965 25.872786185769485 25.53955338187469 26.130711050166173 82-83 19.363429234291868 25.23723171036813 28.351863594143612 27.04747546119639 84-85 20.002959437433525 27.303484514728773 25.646229265181493 27.04732678265621 86-87 20.40387758134007 27.318942207695734 25.02549398306344 27.251686227900755 88-89 19.7275477364926 26.35855459519175 23.622414096895355 30.291483571420297 90-91 21.803958343175857 24.047890075141904 24.816394240497473 29.331757341184773 92-93 22.002434706542687 27.398664328429255 25.870851802615842 24.72804916241222 94-95 19.761884811651356 26.25276555081928 26.56763573112458 27.41771390640479 96-97 16.854499314579336 26.971358820651552 28.87358501526467 27.300556849504442 98-99 21.129092427439076 27.360277426872297 23.250486294827855 28.260143850860768 100 19.09702784839614 23.50227662510385 25.00935965684824 32.39133586965177 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5036.0 1 3146.5 2 1514.5 3 1648.5 4 1301.0 5 1221.0 6 1782.0 7 3043.5 8 4192.0 9 4605.0 10 3814.5 11 2696.0 12 2535.5 13 2591.5 14 2705.0 15 2877.5 16 2983.5 17 3178.5 18 3455.0 19 3743.0 20 4071.5 21 4487.0 22 5068.0 23 5965.0 24 7347.0 25 9077.5 26 10896.5 27 12639.0 28 14709.5 29 16791.5 30 19045.5 31 21212.0 32 22988.5 33 24158.5 34 24043.5 35 23263.5 36 23210.5 37 23381.5 38 22520.0 39 21707.5 40 20702.0 41 19618.0 42 19230.5 43 19608.5 44 20266.0 45 26333.5 46 66903.5 47 244578.5 48 568794.0 49 648121.0 50 566727.5 51 440511.0 52 185752.5 53 50802.0 54 18778.0 55 12127.0 56 9652.5 57 8092.5 58 7031.0 59 6229.5 60 5624.0 61 5199.0 62 4533.5 63 3663.0 64 3142.5 65 2584.0 66 2189.5 67 2023.0 68 1417.0 69 871.5 70 528.5 71 320.0 72 246.5 73 222.5 74 304.0 75 321.0 76 170.0 77 48.5 78 24.0 79 17.5 80 16.5 81 15.0 82 10.5 83 6.5 84 5.5 85 5.5 86 2.5 87 3.0 88 3.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04260863493402941 2 0.009924186937214662 3 0.0 4 2.0799194179791207E-4 5 5.348364217660596E-4 6 2.9713134542558865E-5 7 0.0 8 0.0 9 0.0 10-11 1.4856567271279433E-5 12-13 2.2284850906919147E-4 14-15 0.011692118442496914 16-17 0.0016936486689258553 18-19 1.4856567271279433E-5 20-21 8.616809017342071E-4 22-23 6.091192581224568E-4 24-25 0.004338117643213594 26-27 0.015762817874827476 28-29 0.0130737791987259 30-31 0.009448776784533718 32-33 0.007309431097469481 34-35 0.00864652215188463 36-37 0.004501539883197668 38-39 0.00417469540322952 40-41 0.0051849419776765215 42-43 0.00656660273390551 44-45 0.006863734079331099 46-47 0.0151685551839763 48-49 0.017679315052822525 50-51 0.016802777583817038 52-53 0.011424700231613884 54-55 0.003714141817819858 56-57 0.003981560028702888 58-59 0.009329924246363483 60-61 0.002243341657963194 62-63 1.6342223998407375E-4 64-65 1.1885253817023546E-4 66-67 7.57684930835251E-4 68-69 1.0399597089895604E-4 70-71 1.9313537452663262E-4 72-73 0.0032090185305963573 74-75 0.005927770341240494 76-77 0.0057792046685277 78-79 0.0037884246541762556 80-81 0.0 82-83 8.616809017342071E-4 84-85 3.1198791269686807E-4 86-87 4.4569701813838294E-4 88-89 2.3770507634047092E-4 90-91 4.7541015268094184E-4 92-93 5.051232872235007E-4 94-95 2.9713134542558865E-5 96-97 0.0018867840434524878 98-99 4.6055358540966247E-4 100 2.0799194179791207E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3365515.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.200325622565082 #Duplication Level Percentage of deduplicated Percentage of total 1 72.83617047049687 7.429526558997273 2 10.502181724770745 2.1425134668002754 3 4.283393571449174 1.3107602759515071 4 2.370925494769442 0.9673684829395814 5 1.5611936492787815 0.7962341791262117 6 1.1347892860811932 0.6945132138615799 7 0.894828682660684 0.6389280757684954 8 0.6817347093597772 0.5563132818939596 9 0.5357461629447495 0.49183067817686305 >10 4.284894706701716 8.716779130812077 >50 0.5111811181010382 3.5992703585876265 >100 0.31028539151859363 6.39317472404342 >500 0.041645109256792656 3.0108597893230775 >1k 0.028447715478231602 6.264958830158728 >5k 0.007625160849835275 5.756253446814576 >10k+ 0.014957046282369194 51.230715506744794 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 191942 5.703198470367834 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 125099 3.7170834181395715 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 82999 2.466160453897843 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 78434 2.330519994711062 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 73992 2.1985342510730153 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 57026 1.6944212104239618 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 52494 1.5597612846770852 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 50146 1.4899948447711568 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 49539 1.4719589721038235 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 48628 1.4448903065355525 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 48198 1.4321136586822523 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 44617 1.3257109238853488 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 42648 1.2672057619710506 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 41629 1.236928077872183 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 36417 1.0820632206363663 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 34145 1.0145549789556725 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 33421 0.9930426695468598 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 33291 0.9891799620563272 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 31939 0.9490078041547876 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 29757 0.8841737445829242 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 28617 0.8503007712044072 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 27065 0.8041859863943558 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 26240 0.7796726503967446 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 26096 0.7753939590226162 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 25791 0.7663314529871357 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 24904 0.7399759026478859 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 23255 0.6909789437872065 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 22853 0.6790342637010978 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 22708 0.6747258591924268 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 19718 0.5858835869101757 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 18605 0.5528128681643077 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 18449 0.5481776191756685 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 18403 0.5468108149867108 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 17436 0.5180782138840564 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 14844 0.44106176914974377 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 14639 0.43497057656851923 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14243 0.42320417528966586 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 13963 0.4148844976177494 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13579 0.4034746539534068 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13361 0.396997190623129 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 12836 0.38139779498828563 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 12027 0.3573598691433555 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 11308 0.33599612540725565 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 11265 0.3347184606219256 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 10840 0.3220903784413381 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 10774 0.3201293115615292 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 10640 0.3161477515328263 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 10594 0.31478094734386863 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 10568 0.31400840584576206 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 10303 0.30613442519198397 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 10255 0.3047081947339411 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 9987 0.2967450746765354 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9977 0.2964479433311098 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 9561 0.2840872793614053 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 9418 0.2798383011218194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8677 0.2578208684257833 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 8585 0.25508726004786786 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 8162 0.24251860413636545 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 8061 0.239517577547567 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 8054 0.2393095856057691 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 8030 0.2385964703767477 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8025 0.23844790470403493 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 7918 0.23526859930798108 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7507 0.22305650101098942 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7471 0.2219868281674573 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 7019 0.2085564913542207 RNA PCR Primer, Index 33 (95% over 22bp) CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 6785 0.2016036178712619 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6721 0.19970197726053812 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 6554 0.1947398837919308 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 6521 0.19375935035202638 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 6239 0.18538024641102474 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 6136 0.1823197935531412 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5650 0.1678792101654576 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 5593 0.16618556149653174 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 5363 0.1593515405517432 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5214 0.15492428350490192 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 5192 0.15427059454496564 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4834 0.14363329237872957 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4707 0.13985972429182458 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4573 0.1358781642631217 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 4476 0.13299599021249348 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4467 0.13272857200161045 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4379 0.1301138161618653 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 4095 0.12167528595177855 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 4061 0.12066503937733154 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 4014 0.11926852205383129 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 4003 0.11894167757386313 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3995 0.11870397249752268 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3880 0.1152869620251284 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3827 0.11371216589437277 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3675 0.10919576944390383 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 3565 0.10592732464422235 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 3502 0.10405539716804116 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 3481 0.1034314213426474 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.942626908511773E-5 0.0 10-11 0.0 0.0 0.0 1.6342223998407377E-4 0.0 12-13 0.0 0.0 0.0 1.782788072553532E-4 0.0 14-15 0.0 0.0 0.0 2.2284850906919147E-4 0.0 16-17 0.0 0.0 0.0 4.011273163245447E-4 0.0 18-19 0.0 0.0 0.0 8.61680901734207E-4 0.0 20-21 0.0 0.0 0.0 0.003996416595974168 0.0 22-23 0.0 0.0 0.0 0.01285093068965671 0.0 24-25 0.0 0.0 0.0 0.026102988695637964 0.0 26-27 0.0 0.0 0.0 0.05069060752960543 0.0 28-29 0.0 0.0 0.0 0.14023113847360658 0.0 30-31 0.0 0.0 0.0 0.3229223462085298 0.0 32-33 0.0 0.0 0.0 0.7138283442504342 0.0 34-35 0.0 5.942626908511773E-5 0.0 1.3266023179216258 0.0 36-37 0.0 5.942626908511773E-5 0.0 2.083083866807903 0.0 38-39 0.0 5.942626908511773E-5 0.0 2.9952919538317317 0.0 40-41 0.0 5.942626908511773E-5 0.0 4.077919724024406 0.0 42-43 0.0 5.942626908511773E-5 0.0 5.0152056966021545 0.0 44-45 0.0 5.942626908511773E-5 0.0 5.937917376686777 0.0 46-47 0.0 5.942626908511773E-5 0.0 7.078872029986496 0.0 48-49 0.0 5.942626908511773E-5 0.0 8.015875727786089 0.0 50-51 0.0 5.942626908511773E-5 0.0 8.945109440902804 0.0 52-53 0.0 5.942626908511773E-5 0.0 9.887431789785516 0.0 54-55 0.0 8.91394036276766E-5 0.0 10.908657367445993 0.0 56-57 0.0 1.1885253817023546E-4 0.0 12.192339062520892 0.0 58-59 0.0 1.1885253817023546E-4 0.0 13.233576436295781 0.0 60-61 0.0 1.1885253817023546E-4 0.0 14.237256408008879 0.0 62-63 0.0 1.1885253817023546E-4 0.0 15.139302603019152 0.0 64-65 0.0 1.1885253817023546E-4 0.0 16.094862153340575 0.0 66-67 0.0 1.1885253817023546E-4 0.0 17.20270151819261 0.0 68-69 0.0 1.1885253817023546E-4 0.0 18.52530741951826 0.0 70-71 0.0 1.1885253817023546E-4 0.0 20.014351443984054 0.0 72-73 0.0 1.1885253817023546E-4 0.0 21.143257421226767 0.0 74-75 0.0 1.1885253817023546E-4 0.0 22.34830330573478 0.0 76-77 0.0 1.1885253817023546E-4 0.0 23.418109858372343 0.0 78-79 0.0 1.1885253817023546E-4 0.0 24.94015031874765 0.0 80-81 0.0 1.1885253817023546E-4 0.0 26.345343877534347 0.0 82-83 0.0 1.1885253817023546E-4 0.0 27.644312980331392 0.0 84-85 0.0 1.1885253817023546E-4 0.0 29.06773554715994 0.0 86-87 0.0 1.1885253817023546E-4 0.0 30.33336651300024 0.0 88 0.0 1.1885253817023546E-4 0.0 31.429127488660725 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTCGC 20 0.0021644796 70.49066 7 TACCGTA 70 2.1827873E-11 60.42057 7 GTACCGT 85 1.8189894E-12 55.286797 6 GTACCCA 1620 0.0 53.398655 1 TGTACCG 90 3.6379788E-12 52.21608 5 TACCCAT 1725 0.0 49.586994 2 ACCCATG 1910 0.0 45.02612 3 ACCGTAT 95 4.274625E-10 44.528355 8 TTCGAGT 55 0.002719317 34.1778 5 GCTTATG 1630 0.0 34.0347 1 CTTATGT 1630 0.0 32.87023 2 GCGGCTC 60 0.004161177 31.33477 9 CTAGCGG 30 0.004163438 31.331743 76-77 GGGAGAC 210 0.0 31.329184 7 TATGTAC 1850 0.0 28.704733 4 CCGTATG 150 3.700552E-8 28.201294 9 TCATCCG 150 3.706009E-8 28.197104 94 TTATGTA 1975 0.0 27.36387 3 ATGGGCG 190 4.4929038E-10 27.207327 5 TGGGAGA 575 0.0 25.335775 6 >>END_MODULE