FastQCFastQC Report
Mon 6 Jun 2016
ERR522920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12232331
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400670.32754999844265165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335250.27406877724286566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189960.15529337785251232No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA301350.033.339031
GTATCAA453350.031.747591
TACCTGG123700.029.4236282
GTACCTG143150.026.5158251
TCAACGC534300.026.4966984
CAACGCA539500.026.2308735
ATCAACG544250.026.0073783
TATCAAC553000.025.6806962
AACGCAG554800.025.5960586
ACCTGGG152350.023.365873
GTACATG531200.023.2785261
ACGCAGA625650.022.6598577
TACATGG543200.022.4734572
CATGGGG325400.021.8040854
CGCAGAG648700.021.7886078
ACATGGG551800.021.5353573
GAGTACT341700.020.5855112-13
GCAGAGT702800.019.5959059
GTACTTT376300.018.59388714-15
ATGGGGG179650.018.5809885
Produced by FastQC (version 0.11.5)