FastQCFastQC Report
Mon 6 Jun 2016
ERR522920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12232331
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360130.2944083184145361No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329530.2693926447869993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188560.15414886990876883No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA301150.036.177491
GTATCAA449650.031.3196951
TACCTGG123750.030.1549952
GTACCTG144750.026.3449611
TCAACGC535300.026.0384584
CAACGCA545000.025.5663995
ATCAACG544600.025.5592883
TATCAAC554350.025.2054712
AACGCAG561200.024.9124246
ACCTGGG157800.022.9906713
ACGCAGA640550.021.8264127
GTACATG527300.021.713831
CGCAGAG663800.021.0242658
TACATGG541050.020.8389952
GAGTACT355900.020.5648412-13
ACATGGG543350.019.9184533
CATGGGG328400.019.9051444
TATAACG11100.019.0534462
GCAGAGT727000.018.989679
GTACTTT387250.018.84322414-15
Produced by FastQC (version 0.11.5)