FastQCFastQC Report
Mon 6 Jun 2016
ERR522919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10989245
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270260.24593136289162723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217170.19762049167162984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126490.11510344887205627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141750.052.94421
GTATCAA262700.042.431241
TCAACGC319800.034.2860574
ATCAACG327200.033.4684643
CAACGCA327550.033.4441535
TATCAAC340400.032.286242
AACGCAG341300.032.172216
GTACATG379350.028.7758831
ACGCAGA396500.027.598467
TACATGG391700.027.3737472
CGCAGAG413200.026.436348
ACATGGG403300.025.9062423
CATGGGG281750.024.053744
GCAGAGT468250.022.9856249
ATGGGGG178400.020.3365845
GAGTACT262700.020.0666312-13
GTGGTAT74950.019.9634781
CAGAGTA450150.019.88547310-11
AGAGTAC424750.019.82485610-11
TGGTATC76300.018.2360042
Produced by FastQC (version 0.11.5)