FastQCFastQC Report
Mon 6 Jun 2016
ERR522919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10989245
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242020.22023351012740183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207520.18883917866969022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114840.10450217462619132No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138750.054.892171
GTATCAA255500.041.316971
TCAACGC308750.033.4545064
ATCAACG314900.032.7718933
CAACGCA316700.032.599875
TATCAAC328750.031.616392
AACGCAG329900.031.3520346
GTACATG374900.027.6185681
ACGCAGA386150.026.6754937
TACATGG385550.026.3120822
CGCAGAG401250.025.6426818
ACATGGG395050.024.7549363
CATGGGG280300.022.2475264
GTGGTAT77100.022.2097451
GCAGAGT458200.022.2092329
ATGGGGG170350.020.1930925
GAGTACT255450.020.06616412-13
CAGAGTA439350.019.69110-11
TGGTATC77150.019.6293412
AGAGTAC414650.019.37327410-11
Produced by FastQC (version 0.11.5)