FastQCFastQC Report
Mon 6 Jun 2016
ERR522918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8390605
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT817380.9741609812403277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246950.29431727509518085No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT226970.2705049278329751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193420.23051973010289484No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC172260.20530104801739565No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC168850.20123697873991206No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG137330.16367115362956544No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA131100.15624618248624503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115560.1377254679489739No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA111400.13276754179227837No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC105060.1252114716400069No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG309800.029.4426361
TACATGG320100.028.2435192
ACATGGG331200.026.5323283
CATGGGG218150.025.4729754
ATGGGGG137400.023.0622125
GAGTACT224950.019.94389712-13
GAGTACA235400.019.1543981
AGAGTAC356150.018.72250410-11
GTACTTT240750.018.37164314-15
AGTACAT229500.018.1499822
AGTACTT237750.017.68459712-13
CATGGGA116050.017.0145914
GTATAGC29250.016.3957271
TATAACG5250.016.119012
GTGTAGC40050.015.7310851
TGGGGGG134400.015.4497286
ACTTTTT293750.015.29608516-17
TACTTTT284300.014.59901914-15
ATGGGGA84300.014.3886455
GTATAGA26300.014.1230241
Produced by FastQC (version 0.11.5)