FastQCFastQC Report
Mon 6 Jun 2016
ERR522918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8390605
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT837940.9986645778224574No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT229180.2731388261037196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221310.26375928791785574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184370.2197338570937376No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC177800.21190367083184108No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC168590.2009271083551186No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG140620.1675922058063751No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA134010.1597143471775873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109480.13047926818149586No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC107930.12863196396445786No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG318600.028.9328251
TACATGG326250.027.646552
ACATGGG333250.026.2430933
CATGGGG220550.024.2478924
ATGGGGG136450.022.9715925
GAGTACT221700.019.80808812-13
GAGTACA244200.018.3346161
GTACTTT237350.018.25037214-15
AGAGTAC364900.018.24512910-11
AGTACAT235050.017.7369842
AGTACTT235850.017.60298212-13
CATGGGA118750.017.1353554
TGGGGGG128000.016.2274826
ACTTTTT288000.015.200582516-17
GTATATG33350.014.8072371
GTATAGG25100.014.802461
GTATAGC29400.014.5570921
TACTTTT279250.014.55249414-15
TACCTGG52400.014.1723612
GTGTAGC44350.013.891791
Produced by FastQC (version 0.11.5)