FastQCFastQC Report
Mon 6 Jun 2016
ERR522917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5758421
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT607521.0550114345581887No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT162650.28245590240796914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154020.2674691551729198No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127040.22061603345778294No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC125960.21874051931944538No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC116340.20203455079091992No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG97060.16855315024726394No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA94870.164750024355635No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA85300.1481308851853659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71740.12458276322623858No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG172000.024.6300451
TACATGG177450.023.4200742
ACATGGG176200.022.5470313
CATGGGG117350.020.7106154
TACCTGG42700.019.5976582
GAGTACT162000.018.72088612-13
AGAGTAC236700.017.8634310-11
GTACTTT173200.017.27859114-15
GTACCTG51400.016.9230041
ATGGGGG68100.016.8446645
AGTACTT173300.016.57841712-13
GAGTACA134850.016.073811
GAGTACG9800.015.8327641
TATACTG24850.015.7025915
GTATAGG13500.015.3245521
AGTACAT133150.015.1117462
ATAACGC5251.05501385E-1014.3284513
ATTATAC22100.014.2534863
ACTTTTT212350.014.14703316-17
GTAATAC12700.014.0675493
Produced by FastQC (version 0.11.5)