FastQCFastQC Report
Mon 6 Jun 2016
ERR522917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5758421
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT609821.0590055850379818No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT157900.2742071133736141No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139700.24260122696829564No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC131940.22912531056690716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125020.21710812738422564No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC122110.21205465873370496No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG101100.17556896239437858No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA96750.16801480822607448No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC78470.13626999484754587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70740.1228461760611112No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG180950.023.5702571
TACATGG189550.021.8217532
ACATGGG185300.021.5321623
GAGTACT165500.019.5711412-13
CATGGGG122300.019.3668694
TACCTGG43400.018.628142
GTACTTT173650.018.568814-15
AGAGTAC247000.017.77636310-11
ATGGGGG67950.017.636095
GTACCTG50150.017.4404471
GAGTACA145600.016.5357631
AGTACTT175600.016.45099812-13
TATAACG4451.3460522E-1015.8438982
ACTTTTT209350.015.60186816-17
GTATAGC17400.014.8637721
TATACTG24350.014.8608385
TAACGCA4452.2773747E-914.7850694
AGTACAT146300.014.7468462
GTATAGT15500.014.5621381
TATACCG3555.8857586E-714.5618295
Produced by FastQC (version 0.11.5)