##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522917_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5758421
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3892738999111	34.0	31.0	34.0	31.0	34.0
2	32.67216829057827	34.0	31.0	34.0	30.0	34.0
3	32.73510307773606	34.0	31.0	34.0	30.0	34.0
4	35.54841526869953	37.0	35.0	37.0	33.0	37.0
5	35.7332419772712	37.0	35.0	37.0	34.0	37.0
6	35.87885151155152	37.0	35.0	37.0	34.0	37.0
7	35.8504279211263	37.0	36.0	37.0	35.0	37.0
8	35.85524660319209	37.0	36.0	37.0	34.0	37.0
9	37.17720899531312	39.0	36.0	39.0	35.0	39.0
10-11	37.05517172155353	39.0	36.0	39.0	34.5	39.0
12-13	37.13056539283946	39.0	36.0	39.0	34.5	39.0
14-15	38.233068318554686	40.0	37.0	41.0	34.0	41.0
16-17	38.264281996748764	40.0	37.0	41.0	34.5	41.0
18-19	38.14501457951754	40.0	36.5	41.0	33.5	41.0
20-21	38.144207934779345	39.5	37.0	41.0	34.0	41.0
22-23	37.98559414117169	39.0	36.5	41.0	34.0	41.0
24-25	37.980221400970855	39.0	36.5	41.0	33.5	41.0
26-27	37.75738861399679	39.0	36.5	41.0	34.0	41.0
28-29	37.66723108643845	39.0	37.0	41.0	33.0	41.0
30-31	37.400245657620374	38.5	36.0	41.0	33.0	41.0
32-33	37.29928864179955	38.0	36.5	41.0	32.5	41.0
34-35	37.219684528102405	38.5	36.0	41.0	32.5	41.0
36-37	37.119121457079984	38.0	36.0	40.5	33.0	41.0
38-39	37.10005555342342	38.0	36.0	40.5	32.5	41.0
40-41	37.11964529859835	38.0	36.0	41.0	33.0	41.0
42-43	37.09339973579563	38.0	36.0	41.0	33.0	41.0
44-45	37.12510417352257	38.5	35.5	41.0	32.0	41.0
46-47	36.919358883277205	38.0	35.5	41.0	32.0	41.0
48-49	36.74688521384595	38.0	36.0	41.0	31.5	41.0
50-51	36.635650380547034	38.0	35.5	40.5	31.0	41.0
52-53	36.2823372935046	38.0	35.0	40.0	30.5	41.0
54-55	36.32598042067435	37.5	36.0	40.0	30.0	41.0
56-57	36.20540587428394	37.5	35.5	40.0	30.0	41.0
58-59	35.94110477507637	37.5	35.5	40.0	29.5	41.0
60-61	35.700119529294575	37.0	35.0	40.0	29.0	41.0
62-63	35.50314391740375	36.5	35.0	40.0	29.0	41.0
64-65	35.213270964384165	37.0	34.5	39.0	28.5	41.0
66-67	34.93546512142825	36.5	34.0	39.0	28.5	41.0
68-69	34.7407686933623	36.5	34.0	38.5	28.0	40.0
70-71	34.347982719568435	36.0	34.0	37.5	27.5	40.0
72-73	34.06168444439891	36.0	34.0	37.0	26.5	39.5
74-75	33.80257018373613	35.5	33.5	37.0	26.0	39.0
76-77	32.277045565095015	34.5	31.0	36.5	24.5	37.5
78-79	33.205567811037085	35.0	33.0	36.0	26.0	38.0
80-81	33.236993960670816	35.0	33.0	36.0	26.0	37.5
82-83	33.056947295100514	35.0	33.0	36.0	26.0	37.5
84-85	32.991360478853494	35.0	33.0	36.5	26.0	37.0
86-87	32.68747908497833	35.0	33.0	36.0	26.0	36.5
88-89	32.683478682784745	35.0	33.0	35.5	25.5	37.0
90-91	32.57148635016439	35.0	33.0	36.0	25.0	37.0
92-93	32.34194165379711	35.0	33.0	35.0	24.0	37.0
94-95	32.2501932387368	35.0	32.5	35.0	25.0	36.0
96-97	32.00413472721081	35.0	32.0	35.0	24.0	36.5
98-99	31.932071569619517	35.0	32.0	35.0	24.0	36.0
100	30.780407337358625	34.0	30.0	35.0	20.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.12100030559619768
1101	2	-0.14583764600378402
1101	3	-0.2072252617628294
1101	4	-0.06183059412531833
1101	5	-0.10396463439327874
1101	6	-0.06610186511353788
1101	7	-0.13097460397076333
1101	8	-0.05733828943297681
1101	9	-0.18955984270701265
1101	10-11	-0.15736877184941278
1101	12-13	-0.1007529120203472
1101	14-15	-0.14386585571319443
1101	16-17	-0.14634341941673767
1101	18-19	-0.2788978813018659
1101	20-21	-0.15218361175988093
1101	22-23	-0.22494820046500053
1101	24-25	-0.2554913722762464
1101	26-27	-0.3637025632274984
1101	28-29	-0.24071198096380897
1101	30-31	-0.22236730566515917
1101	32-33	-0.22687672012312987
1101	34-35	-0.20871027950550314
1101	36-37	-0.204032456187349
1101	38-39	-0.2978453239258343
1101	40-41	-0.14187749159319907
1101	42-43	-0.26057164892269213
1101	44-45	-0.31674949961993093
1101	46-47	-0.3147453577083539
1101	48-49	-0.31166820563728237
1101	50-51	-0.2765910260239295
1101	52-53	-0.2815165343298389
1101	54-55	-0.38815540506025314
1101	56-57	-0.330057767012903
1101	58-59	-0.3817230545051231
1101	60-61	-0.35248581548285074
1101	62-63	-0.3572524834674269
1101	64-65	-0.40339162283038377
1101	66-67	-0.46355901328752935
1101	68-69	-0.5806198735107486
1101	70-71	-0.5303685927333035
1101	72-73	-0.4194836814346985
1101	74-75	-0.604116700769012
1101	76-77	-0.5347816388656241
1101	78-79	-0.33616835204565376
1101	80-81	-0.16218795866683422
1101	82-83	-0.5966544776911675
1101	84-85	-0.4506279396958064
1101	86-87	-0.4645851909353311
1101	88-89	-0.5596767347269562
1101	90-91	-0.4509840133666785
1101	92-93	-0.4013584370255252
1101	94-95	-0.7219241921193493
1101	96-97	-0.4181515211576752
1101	98-99	-0.5265277487032947
1101	100	-0.27448812199395434
1102	1	-0.11862395211503696
1102	2	-0.16788098241637783
1102	3	-0.17240060009962122
1102	4	-0.11929042224644348
1102	5	-0.2103091080685573
1102	6	-0.16538875060842173
1102	7	-0.25857780743700687
1102	8	-0.1979534154653777
1102	9	-0.3359983699098521
1102	10-11	-0.23849743403358303
1102	12-13	-0.15068973848112677
1102	14-15	-0.263550180595125
1102	16-17	-0.3589370207829674
1102	18-19	-0.46128780061563646
1102	20-21	-0.35422095839975754
1102	22-23	-0.25337922149194014
1102	24-25	-0.3254178987897731
1102	26-27	-0.2767137214375097
1102	28-29	-0.3128003449021648
1102	30-31	-0.3112884298682701
1102	32-33	-0.2744610668239602
1102	34-35	-0.2561530217850603
1102	36-37	-0.2901315179352153
1102	38-39	-0.15906792845849083
1102	40-41	-0.22991742235246448
1102	42-43	-0.2086641237073792
1102	44-45	-0.28087161474626754
1102	46-47	-0.3175436953429269
1102	48-49	-0.37367352079125027
1102	50-51	-0.25618624087650943
1102	52-53	-0.2831278864282183
1102	54-55	-0.3692640947175576
1102	56-57	-0.40247128011485955
1102	58-59	-0.39999667574064546
1102	60-61	0.0579326863830687
1102	62-63	-0.330327641118366
1102	64-65	-0.30419586447095526
1102	66-67	-0.9385543427736707
1102	68-69	-0.3469964024960248
1102	70-71	-0.375996991760573
1102	72-73	-0.36039026540001373
1102	74-75	-0.3679059338299311
1102	76-77	-0.43346126821841224
1102	78-79	-0.35479923532965785
1102	80-81	-0.3964179512476136
1102	82-83	-0.20676529146442135
1102	84-85	-0.3071500020914968
1102	86-87	-0.2934108083415268
1102	88-89	-0.3700347631169336
1102	90-91	-0.36075430151306165
1102	92-93	-0.4910951391642726
1102	94-95	-1.2855949599041807
1102	96-97	-0.549124195127618
1102	98-99	-0.6311862214720669
1102	100	-0.5911806278690719
1103	1	-0.13238055344831423
1103	2	-0.17383866735643494
1103	3	-0.16228419748627942
1103	4	-0.22168089245508327
1103	5	-0.2198416387736799
1103	6	-0.18124525586894435
1103	7	-0.27277974806757044
1103	8	-0.1947352716066959
1103	9	-0.14026274804909633
1103	10-11	-0.17006183506592265
1103	12-13	-0.23312896903813396
1103	14-15	-0.30134610327514366
1103	16-17	-0.3856653031546955
1103	18-19	-0.320634838502734
1103	20-21	-0.18338999753521534
1103	22-23	-0.27803871107362
1103	24-25	-0.3674734057545592
1103	26-27	-0.2767106885907964
1103	28-29	-0.32701666709613875
1103	30-31	-0.2951356839679917
1103	32-33	-0.26442792074445265
1103	34-35	-0.2753540721283585
1103	36-37	-0.3777004662809489
1103	38-39	-0.3340310940460469
1103	40-41	-0.4003053919300683
1103	42-43	-0.436177424207699
1103	44-45	-0.29804506031448597
1103	46-47	-0.3313187872577501
1103	48-49	-0.37197405046324405
1103	50-51	-0.26847523790431893
1103	52-53	-0.24909308500140526
1103	54-55	-0.3042704832623997
1103	56-57	-0.4307374114351674
1103	58-59	-0.43823050235182137
1103	60-61	-0.4858987969137729
1103	62-63	-0.31788504507985493
1103	64-65	-0.28477273148160975
1103	66-67	-0.20215284474306117
1103	68-69	-0.3486584024921129
1103	70-71	-0.3724657798545934
1103	72-73	-0.4147228336733093
1103	74-75	-0.35838592584669726
1103	76-77	-0.3995602055480596
1103	78-79	-0.3655157502561579
1103	80-81	-0.38320374030627846
1103	82-83	-0.08040055936262291
1103	84-85	-0.32182105562417007
1103	86-87	-0.25371818246371447
1103	88-89	-0.3121276425798669
1103	90-91	-0.21635861089472996
1103	92-93	-0.20576364901906885
1103	94-95	-0.8735992685031775
1103	96-97	-0.2513812200810932
1103	98-99	-0.2659326657038861
1103	100	-0.2404068606234233
1104	1	-0.09264796121847496
1104	2	-0.2298526891444581
1104	3	-0.18522369785942772
1104	4	-0.12019824849095073
1104	5	-0.15925265126450938
1104	6	-0.12356883880970315
1104	7	-0.17063574788778624
1104	8	-0.1604013986136863
1104	9	-0.19074162188639576
1104	10-11	-0.22694831707471508
1104	12-13	-0.14433698896153402
1104	14-15	-0.23654163705288056
1104	16-17	-0.2876603272989229
1104	18-19	-0.29493854798417374
1104	20-21	-0.27274549905197887
1104	22-23	-0.9808480626361487
1104	24-25	-0.3189327185670905
1104	26-27	-0.2288265197213306
1104	28-29	-0.19271669059896368
1104	30-31	-0.13833620608639308
1104	32-33	-0.27104106153569774
1104	34-35	-0.16475275578321913
1104	36-37	-0.336543706224127
1104	38-39	-0.2620467351252316
1104	40-41	-0.11351346116881444
1104	42-43	-0.09831926762149124
1104	44-45	-0.24902097351449726
1104	46-47	-0.20065386660395035
1104	48-49	-0.2284477661652815
1104	50-51	-0.21663551027483408
1104	52-53	-0.08953811228246877
1104	54-55	-0.10761020886308614
1104	56-57	-0.45604039648043937
1104	58-59	-0.3473162770287672
1104	60-61	-0.342534956734184
1104	62-63	-0.30140413807703936
1104	64-65	-0.4525132865788706
1104	66-67	-0.3567099258490174
1104	68-69	-0.4958542789802749
1104	70-71	-0.30121383182047623
1104	72-73	-0.2553485798409838
1104	74-75	-0.7548945243514424
1104	76-77	-0.48082622718251145
1104	78-79	-0.2655979789101863
1104	80-81	-0.18252240825514576
1104	82-83	-0.16568289438796313
1104	84-85	-0.20391128335181463
1104	86-87	-0.13940607376770942
1104	88-89	-0.1430082866507476
1104	90-91	-0.3707506572661359
1104	92-93	-0.2519971428145382
1104	94-95	-0.2517373239991727
1104	96-97	-0.5349084126553265
1104	98-99	-0.2855961446641828
1104	100	-0.3421534041016123
1105	1	-0.031527193367296036
1105	2	-0.15358720799406456
1105	3	-0.1388331237882383
1105	4	-0.0614210355629865
1105	5	-0.0596128838435348
1105	6	-0.11390542611330545
1105	7	-0.14524051585837583
1105	8	-0.08776376683546516
1105	9	0.016695052420161005
1105	10-11	-0.05863448827710016
1105	12-13	-0.07805633623761565
1105	14-15	-0.8244387268617075
1105	16-17	-0.1591208477597661
1105	18-19	-0.23684382036056917
1105	20-21	-0.1612065231739308
1105	22-23	-0.15967873696600066
1105	24-25	-0.1532728330896873
1105	26-27	-0.14196649250848026
1105	28-29	-0.049407649859823266
1105	30-31	-0.15486272537442147
1105	32-33	-0.09539954603579304
1105	34-35	-0.09351752071389541
1105	36-37	-0.24054781202007547
1105	38-39	-0.18194794295077088
1105	40-41	-0.14101232008648168
1105	42-43	-0.2670451495073962
1105	44-45	-0.1710407818508486
1105	46-47	-0.13823518324014827
1105	48-49	-0.14707255620836435
1105	50-51	-0.17150981745438543
1105	52-53	-0.057803675413836686
1105	54-55	-0.15297392022358025
1105	56-57	-0.12841516400740005
1105	58-59	-0.20036434964507777
1105	60-61	-0.2434074908840671
1105	62-63	-0.11521027635209435
1105	64-65	-0.32977911710317187
1105	66-67	-0.26126824662900816
1105	68-69	-0.04016815004138152
1105	70-71	-0.10160540239340321
1105	72-73	-0.009645341191230727
1105	74-75	-0.1266569677818623
1105	76-77	-0.25685798933888293
1105	78-79	-0.09185483550857754
1105	80-81	-0.1061538780296516
1105	82-83	-0.11214487402116902
1105	84-85	-0.20608696184786623
1105	86-87	-0.23460602684505005
1105	88-89	-0.30844993277283805
1105	90-91	-0.1452617613290883
1105	92-93	-0.14389368243423917
1105	94-95	-0.029515542021606223
1105	96-97	-0.04556466722817021
1105	98-99	-0.25913640104951696
1105	100	-0.25008462485888927
1106	1	-0.09997570203089623
1106	2	-0.18438275254018066
1106	3	-0.152265800571449
1106	4	-0.0983574441239412
1106	5	-0.12239844336439631
1106	6	-0.04399985991278044
1106	7	-0.12435448297119223
1106	8	-0.11854296699703326
1106	9	-0.13127534878633895
1106	10-11	-0.16139540325348634
1106	12-13	-0.11152064003841389
1106	14-15	-0.13641717153260657
1106	16-17	-0.2509546864386607
1106	18-19	-0.25127321952452775
1106	20-21	-0.14240405088938246
1106	22-23	-0.16518115637837383
1106	24-25	-0.18703893785161796
1106	26-27	-0.10276055845794474
1106	28-29	-0.10241559310401271
1106	30-31	-0.09627957319135305
1106	32-33	-0.15605238509972708
1106	34-35	-0.10357767945207996
1106	36-37	-0.20104609528954143
1106	38-39	-0.18181563579138782
1106	40-41	-0.16588815661172163
1106	42-43	-0.19177754822067783
1106	44-45	-0.1911642351684364
1106	46-47	-0.2935851057436949
1106	48-49	-0.3016182463018495
1106	50-51	-0.20718545946878208
1106	52-53	-0.11978411562006386
1106	54-55	-0.28050028377919745
1106	56-57	-0.21240778420102657
1106	58-59	-0.2606597028098463
1106	60-61	-0.2534651789594733
1106	62-63	-0.2552598593865767
1106	64-65	-0.27992522829896416
1106	66-67	-0.231385548629774
1106	68-69	0.07614745444935522
1106	70-71	-0.22257009738248712
1106	72-73	-0.20826984086617983
1106	74-75	-0.33844496869765806
1106	76-77	-0.30778557117199057
1106	78-79	-0.20819441809833705
1106	80-81	-0.07068038691092937
1106	82-83	-0.05160273965487505
1106	84-85	-0.16428524812014444
1106	86-87	-0.14343625797618387
1106	88-89	-0.21960290366880741
1106	90-91	-0.18780366028557438
1106	92-93	-0.11555654237971424
1106	94-95	-0.08989128675222702
1106	96-97	-0.06136718474485292
1106	98-99	-0.1707812872400183
1106	100	-0.19897478413301783
1107	1	-0.015520184248337898
1107	2	-0.13608646744800978
1107	3	-0.0928455079611652
1107	4	-0.10408606085368888
1107	5	-0.10125573562707757
1107	6	-0.026766125945904662
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2316	88-89	0.05001047505697187
2316	90-91	0.06125329126960111
2316	92-93	0.054698437913607734
2316	94-95	0.1331376859319846
2316	96-97	0.23747804512088067
2316	98-99	0.21604805604665955
2316	100	0.0754789585872011
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	15.0
13	87.0
14	367.0
15	1063.0
16	2494.0
17	4826.0
18	8604.0
19	13063.0
20	18161.0
21	24898.0
22	33299.0
23	38950.0
24	41990.0
25	45167.0
26	51295.0
27	60811.0
28	73607.0
29	90557.0
30	111959.0
31	140619.0
32	181311.0
33	244068.0
34	378193.0
35	771761.0
36	1071826.0
37	1026811.0
38	1101915.0
39	220631.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52088844005191	18.72747766728102	13.15240809011661	23.599225802550457
2	15.9405489017484	21.487785027318644	38.347118576714	24.22454749421895
3	20.840956227410256	24.874944016771263	29.660144682023077	24.623955073795404
4	13.031397562195043	16.446908564687586	37.9633879476804	32.558305925436976
5	13.501917800701893	37.79386321141271	34.4651544113063	14.239064576579091
6	31.20559087022271	36.69108437986464	17.24284643549549	14.860478314417161
7	27.261917806982154	32.23645857084781	22.150204022943097	18.35141959922694
8	25.58376842198491	33.61086967658579	20.12538194170087	20.67997995972843
9	25.57007400816092	16.693279874215474	20.847336742949757	36.88930937467384
10-11	25.37156712147068	25.253748232260808	28.265636313606812	21.109048332661708
12-13	26.888822705553522	23.171254590021732	27.57277827318802	22.36714443123672
14-15	23.087102963787316	24.97870416967538	26.635554071522204	25.298638795015094
16-17	22.420325465720722	27.6714458854672	26.980282314343405	22.927946334468675
18-19	22.16766540688845	27.850099879810802	27.90194568962568	22.080289023675068
20-21	23.43175972552007	25.951078957783448	27.605693336373538	23.01146798032294
22-23	22.704011681105676	26.036150229605344	27.853925050522204	23.405913038766773
24-25	23.560829974182372	26.045627723091748	27.667337208857063	22.726205093868813
26-27	23.12854117073524	25.428991448176063	28.845943599696035	22.596523781392662
28-29	22.304979011700304	27.443109290253336	27.827038001240016	22.424873696806337
30-31	23.052892035954585	25.98247945610508	27.66413733008168	23.30049117785866
32-33	23.03607351641087	26.222782091661145	27.94978750326606	22.791356888661923
34-35	23.833627666341854	25.934330019170865	27.808582066288924	22.423460248198356
36-37	23.400741784133768	25.60513306145812	28.46808916344623	22.526035990961883
38-39	22.76217298791265	26.591875977123426	28.15881617684409	22.487134858119834
40-41	23.049908379464878	26.697797590470667	27.591313801917472	22.660980228146986
42-43	22.608791845806227	26.808094662447147	26.865924099645156	23.71718939210147
44-45	23.086835856618123	25.919112252034065	27.294448484023327	23.69960340732449
46-47	23.760408983364904	26.56396348174252	27.18473808186927	22.490889453023303
48-49	23.34598147952916	26.531572284355242	27.18297757635999	22.939468659755605
50-51	22.207186181509027	26.826564147944104	28.109918156677704	22.85633151386916
52-53	23.199435351262508	27.279079792063655	26.53908983736477	22.982395019309067
54-55	22.582321022181365	26.341763260943956	27.195149832817677	23.880765884057
56-57	23.343758270658675	26.191660814253076	26.979583238687475	23.484997676400777
58-59	24.262697325993514	26.160567858103896	26.61917786237688	22.957556953525707
60-61	23.16835054929187	26.579629282148233	27.89581806027176	22.356202108288137
62-63	22.620337975527992	26.70559604874673	27.642084125983104	23.031981849742174
64-65	22.40307564173987	27.61750296262439	26.879113115986225	23.100308279649514
66-67	22.951795292494246	26.564539133210303	26.4360403659267	24.047625208368753
68-69	22.56726360543258	26.686909531845394	26.90453914558843	23.8412877171336
70-71	23.391809666507942	26.432551206081968	26.677037606639903	23.498601520770187
72-73	23.01427172191503	25.925899233099003	27.665510642140656	23.39431840284531
74-75	22.304544030068286	27.737904915923995	26.66930469260355	23.28824636140417
76-77	22.724744685327614	26.34914454573391	26.676385662417523	24.249725106520952
78-79	22.88143246199282	26.587278018649403	26.971917181840187	23.559372337517587
80-81	23.43445429629144	26.341432706405072	26.73420027464381	23.489912722659678
82-83	22.965162042112237	26.18323716060419	27.45196175435382	23.399639042929753
84-85	22.583841329728866	26.90963837741931	27.086623088642398	23.419897204209427
86-87	22.554952351951375	27.134975641323866	26.665463211656736	23.644608795068024
88-89	22.256450438463276	26.958393180614017	26.222156579067786	24.56299980185492
90-91	22.804020099629856	26.193130070785976	26.421300515966678	24.58154931361749
92-93	23.27736743645281	26.7673540516991	26.595258159034145	23.360020352813944
94-95	22.8873580640198	26.42092273452063	26.796217564374587	23.895501637084983
96-97	21.863239021335488	26.503258374214063	27.6632241385134	23.97027846593705
98-99	22.438506076433136	26.991430797015152	26.360677579103125	24.20938554744859
100	22.31226233719278	25.541880317538435	26.55253931589927	25.59331802936951
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	505.0
1	405.0
2	562.5
3	778.0
4	637.0
5	653.5
6	1007.0
7	1830.5
8	2930.0
9	3677.5
10	3589.5
11	3332.5
12	3514.0
13	3856.0
14	4300.5
15	4671.0
16	4987.0
17	5464.5
18	6049.5
19	6671.0
20	7412.0
21	8489.0
22	9922.5
23	11904.5
24	14701.5
25	18416.5
26	23303.0
27	29169.0
28	35749.5
29	43500.0
30	52249.5
31	62340.0
32	74979.0
33	84809.0
34	93218.0
35	102745.0
36	114878.5
37	127203.0
38	136275.0
39	144037.5
40	150838.5
41	158974.0
42	166606.0
43	174593.0
44	183289.0
45	193838.0
46	205763.0
47	247545.0
48	330553.5
49	325022.0
50	246954.0
51	216662.0
52	210043.0
53	206933.0
54	203199.0
55	190959.5
56	173442.0
57	157316.5
58	141865.0
59	124950.5
60	106730.5
61	90553.0
62	74016.5
63	55669.5
64	40681.0
65	30386.0
66	22424.5
67	17252.0
68	13374.0
69	10241.5
70	8214.5
71	6761.5
72	5404.0
73	3918.0
74	2720.5
75	1958.0
76	1429.0
77	966.0
78	640.0
79	424.5
80	279.5
81	180.0
82	115.5
83	77.5
84	63.5
85	47.5
86	29.0
87	19.0
88	11.0
89	4.5
90	3.0
91	3.5
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.057307376449203695
2	0.014153185395788186
3	0.0
4	7.640983526560494E-4
5	3.4731743302547696E-5
6	5.73073764492037E-4
7	0.0
8	2.7785394642038157E-4
9	3.4731743302547696E-5
10-11	1.1287816573328E-4
12-13	1.0419522990764308E-4
14-15	0.014561283379593122
16-17	0.0015281967053120988
18-19	0.0
20-21	6.946348660509539E-4
22-23	0.0010766840423789784
24-25	0.002526734325260345
26-27	0.00914313142439568
28-29	0.007832008114724504
30-31	0.0056178594791870895
32-33	0.005774152324048554
34-35	0.0035426378168598653
36-37	0.002465953774480886
38-39	0.002231514507188689
40-41	0.0027698565283781787
42-43	0.0018755141383375758
44-45	0.0020839045981528616
46-47	0.009273375461780234
48-49	0.013753770347808888
50-51	0.01343250172226032
52-53	0.008769765183893292
54-55	0.003238735062962572
56-57	0.001962343496593945
58-59	0.0026309295551679877
60-61	9.117082616918769E-4
62-63	7.9014716013296E-4
64-65	2.2575633146656E-4
66-67	0.0
68-69	2.3443926729219697E-4
70-71	3.994150479792985E-4
72-73	0.003143222768880566
74-75	0.005687322965792186
76-77	0.004888492869833588
78-79	0.0029001005657627327
80-81	9.290741333431509E-4
82-83	0.002057855790675951
84-85	2.4312220311783385E-4
86-87	8.682935825636924E-6
88-89	2.4312220311783385E-4
90-91	1.1287816573328E-4
92-93	6.078055077945846E-5
94-95	2.604880747691077E-4
96-97	0.001814733587558117
98-99	0.0011721963364609849
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5758421.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.4160896425366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.91085604987103	45.862370839507
2	14.486360218214164	17.50418475075406
3	4.503381275306688	8.16230060470349
4	1.8837738967649282	4.552410104528816
5	0.9229939395938503	2.7881842297010038
6	0.578542114065566	2.097195133522072
7	0.3637640400530345	1.5384040596802784
8	0.26227681065110686	1.2676591442764698
9	0.1864239602732862	1.0136706025848787
>10	0.8580194699222589	8.7362794436891
>50	0.03234455703825971	1.3258618320683961
>100	0.009979985295756956	1.0220703056007383
>500	6.24494780469603E-4	0.26553199797057936
>1k	2.7755291352026565E-4	0.33233391414077346
>5k	1.387764567590855E-4	0.6230396255995254
>10k+	2.428587993283996E-4	2.9085034116728754
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	60982	1.0590055850379818	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	15790	0.2742071133736141	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13970	0.24260122696829564	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	13194	0.22912531056690716	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12502	0.21710812738422564	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	12211	0.21205465873370496	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	10110	0.17556896239437858	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	9675	0.16801480822607448	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	7847	0.13626999484754587	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7074	0.1228461760611112	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.4731743302547696E-5
2	0.0	1.7365871651273848E-5	0.0	0.0	3.4731743302547696E-5
3	0.0	1.7365871651273848E-5	0.0	0.0	3.4731743302547696E-5
4	0.0	1.7365871651273848E-5	0.0	1.7365871651273848E-5	3.4731743302547696E-5
5	0.0	1.7365871651273848E-5	0.0	1.7365871651273848E-5	3.4731743302547696E-5
6	0.0	1.7365871651273848E-5	0.0	1.7365871651273848E-5	5.209761495382154E-5
7	0.0	1.7365871651273848E-5	0.0	1.7365871651273848E-5	5.209761495382154E-5
8	0.0	1.7365871651273848E-5	0.0	1.7365871651273848E-5	5.209761495382154E-5
9	0.0	1.7365871651273848E-5	0.0	3.4731743302547696E-5	5.209761495382154E-5
10-11	0.0	1.7365871651273848E-5	0.0	3.4731743302547696E-5	6.946348660509539E-5
12-13	0.0	1.7365871651273848E-5	0.0	6.078055077945847E-5	2.691710105947446E-4
14-15	0.0	1.7365871651273848E-5	0.0	8.682935825636924E-5	3.646833046767508E-4
16-17	0.0	1.7365871651273848E-5	0.0	1.3024403738455385E-4	4.5151266293312E-4
18-19	0.0	1.7365871651273848E-5	0.0	1.5629284486146463E-4	5.383420211894893E-4
20-21	0.0	1.7365871651273848E-5	0.0	1.9102458816401232E-4	7.120007377022278E-4
22-23	0.0	1.7365871651273848E-5	0.0	3.7336624050238773E-4	8.422447750867816E-4
24-25	0.0	1.7365871651273848E-5	0.0	6.7726899439968E-4	8.509277109124185E-4
26-27	0.0	1.7365871651273848E-5	0.0	0.0010332693632507939	8.682935825636924E-4
28-29	0.0	1.7365871651273848E-5	0.0	0.0023096609296194216	8.856594542149662E-4
30-31	0.0	1.7365871651273848E-5	0.0	0.0073891783876170215	9.0302532586624E-4
32-33	0.0	1.7365871651273848E-5	0.0	0.023574170766604247	9.724888124713354E-4
34-35	1.7365871651273848E-5	1.7365871651273848E-5	0.0	0.04803400098742346	0.0010072205557738832
36-37	1.7365871651273848E-5	1.7365871651273848E-5	0.0	0.08534457623018532	0.0010072205557738832
38-39	1.7365871651273848E-5	1.7365871651273848E-5	0.0	0.13891829027436514	0.0010766840423789784
40-41	1.7365871651273848E-5	1.7365871651273848E-5	0.0	0.19504478745128223	0.0010940499140302525
42-43	1.7365871651273848E-5	2.6048807476910774E-5	0.0	0.25280367656341907	0.0011114157856815263
44-45	1.7365871651273848E-5	3.4731743302547696E-5	0.0	0.3315839533094228	0.0011548304648097108
46-47	1.7365871651273848E-5	3.4731743302547696E-5	0.0	0.4297966404332021	0.0011895622081122587
48-49	1.7365871651273848E-5	3.4731743302547696E-5	0.0	0.5276793759956071	0.0012763915663686278
50-51	1.7365871651273848E-5	6.946348660509539E-5	0.0	0.6308847512191276	0.0014066356037531816
52-53	1.7365871651273848E-5	6.946348660509539E-5	0.0	0.7348629077311297	0.0016410748710453786
54-55	1.7365871651273848E-5	6.946348660509539E-5	0.0	0.8586121091181071	0.0016497578068710155
56-57	1.7365871651273848E-5	6.946348660509539E-5	0.0	1.0144621242524643	0.0016758066143479262
58-59	1.7365871651273848E-5	6.946348660509539E-5	0.0	1.1782222939239766	0.0017279042293017479
60-61	1.7365871651273848E-5	7.814642243073231E-5	0.0	1.353990963842345	0.0017626359726042955
62-63	1.7365871651273848E-5	8.682935825636924E-5	0.0	1.5329896858878502	0.0019449776249426708
64-65	1.7365871651273848E-5	8.682935825636924E-5	0.0	1.7228333947795758	0.0020057581757221294
66-67	1.7365871651273848E-5	8.682935825636924E-5	0.0	1.948346604043018	0.002040489919024677
68-69	1.7365871651273848E-5	8.682935825636924E-5	0.0	2.2117781940570165	0.0020839045981528616
70-71	1.7365871651273848E-5	8.682935825636924E-5	0.0	2.4930184854493964	0.0021012704698041354
72-73	1.7365871651273848E-5	8.682935825636924E-5	0.0	2.772921604724629	0.002300977993793785
74-75	1.7365871651273848E-5	8.682935825636924E-5	0.0	3.06952200959256	0.0024659537744808864
76-77	1.7365871651273848E-5	8.682935825636924E-5	0.0	3.394072784883217	0.002518051389434708
78-79	1.7365871651273848E-5	8.682935825636924E-5	0.0	3.789893097430702	0.002613563683516714
80-81	1.7365871651273848E-5	8.682935825636924E-5	0.0	4.199684253721637	0.0026569783626448984
82-83	1.7365871651273848E-5	8.682935825636924E-5	0.0	4.605064478613148	0.0027872224000294525
84-85	1.7365871651273848E-5	8.682935825636924E-5	0.0	5.025075450370857	0.0028653688224601847
86-87	1.7365871651273848E-5	8.682935825636924E-5	0.0	5.464892198746844	0.0029956128598447387
88	1.7365871651273848E-5	8.682935825636924E-5	0.0	5.819859298234707	0.003143222768880566
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	18095	0.0	23.570257	1
TACATGG	18955	0.0	21.821753	2
ACATGGG	18530	0.0	21.532162	3
GAGTACT	16550	0.0	19.57114	12-13
CATGGGG	12230	0.0	19.366869	4
TACCTGG	4340	0.0	18.62814	2
GTACTTT	17365	0.0	18.5688	14-15
AGAGTAC	24700	0.0	17.776363	10-11
ATGGGGG	6795	0.0	17.63609	5
GTACCTG	5015	0.0	17.440447	1
GAGTACA	14560	0.0	16.535763	1
AGTACTT	17560	0.0	16.450998	12-13
TATAACG	445	1.3460522E-10	15.843898	2
ACTTTTT	20935	0.0	15.601868	16-17
GTATAGC	1740	0.0	14.863772	1
TATACTG	2435	0.0	14.860838	5
TAACGCA	445	2.2773747E-9	14.785069	4
AGTACAT	14630	0.0	14.746846	2
GTATAGT	1550	0.0	14.562138	1
TATACCG	355	5.8857586E-7	14.561829	5
>>END_MODULE