FastQCFastQC Report
Mon 6 Jun 2016
ERR522916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5483182
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT197230.3596998968846921No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196270.35794908868609504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152350.2778496136002781No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87090.15883113126647994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG82800.049.753642
ACCTGGG93400.042.64733
GTACCTG118550.035.1465951
CCTGGGG82100.031.3895074
TATAACG6250.030.0975462
ATAACGC7150.026.3092823
TAACGCA7700.026.2618224
GTACATG213450.024.9843441
TACATGG216050.024.2918722
TATCACG4400.023.5137062
CATGGGG129350.023.4135114
ACATGGG213300.023.2162763
ATGGGGG82400.021.4000845
TACCGGG6800.020.7473872
CTGGGGG54050.020.2708365
GTACCGG8500.019.9174921
GAGTACT172200.019.78936812-13
GTACACG11350.019.4739061
TGGGGGG96600.019.0762716
AGTACTT177800.018.14829612-13
Produced by FastQC (version 0.11.5)