FastQCFastQC Report
Mon 6 Jun 2016
ERR522916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5483182
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT203460.3710619125901712No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT201200.3669402182893072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183440.334550266615261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152520.278159652552113No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT107470.19599933031586403RNA PCR Primer, Index 26 (100% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85670.1562413941393884No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT60140.109680838607947RNA PCR Primer, Index 26 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG83150.053.3632432
ACCTGGG91700.047.4642073
GTACCTG123800.036.5083961
TATAACG6550.035.1631132
CCTGGGG84500.034.320144
TAACGCA7350.030.0559964
ATAACGC7600.028.449123
GTACATG210000.024.1429021
CTGGGGG50500.022.9897425
TACATGG214950.022.9169242
TACCGGG6200.022.743752
GTACACG10800.022.6448571
TGGGGGG76950.022.5984176
CATGGGG120000.022.5611824
ACATGGG209400.021.9526393
ATGGGGG73750.021.924265
GTATAAC17050.020.6883981
GAGTACT173900.020.62044312-13
GTACTTT190950.018.5454214-15
TATCACG3851.4551915E-1118.3131482
Produced by FastQC (version 0.11.5)