FastQCFastQC Report
Mon 6 Jun 2016
ERR522915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5506051
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176410.32039296403175344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152670.2772767633282002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77510.1407723974950468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93650.050.839511
GTATCAA165350.040.061491
TCAACGC198250.032.7837834
ATCAACG200800.032.3458183
TATCAAC204500.031.9936082
CAACGCA203050.031.9622145
AACGCAG210250.030.9517216
ACGCAGA235900.027.3477
CGCAGAG244850.026.2888588
GTACATG181200.026.2755011
TACATGG185650.025.1403642
ACATGGG189450.023.7653773
GCAGAGT270950.023.5644829
GAGTACT160650.021.67119412-13
CATGGGG131950.021.0132244
CAGAGTA262550.020.88154810-11
AGAGTAC242950.019.89767510-11
GTGGTAT50500.019.5639511
GTACTTT183250.018.9353614-15
TACCTGG41700.018.5979542
Produced by FastQC (version 0.11.5)