FastQCFastQC Report
Mon 6 Jun 2016
ERR522915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5506051
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163870.2976180206104157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151720.2755513888265837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74980.1361774527696892No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100000.050.155971
GTATCAA173300.038.6070371
TCAACGC204900.032.1316344
ATCAACG206750.031.889873
CAACGCA208250.031.6373165
TATCAAC216200.030.6723652
AACGCAG215650.030.6382986
ACGCAGA248800.026.518297
CGCAGAG256550.025.7216478
GTACATG187450.024.5732561
TACATGG188150.024.2216322
TACCTGG44400.023.515482
GCAGAGT286000.022.8758349
ACATGGG189850.022.8718853
GAGTACT169500.021.7674512-13
GTGGTAT54300.021.0558071
TAACGCA5050.020.4723634
CAGAGTA278100.020.44148810-11
TGGTATC52950.020.4289342
CATGGGG131350.020.3572374
Produced by FastQC (version 0.11.5)