##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522915_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5506051
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67256078812201	34.0	31.0	34.0	30.0	35.0
2	32.733486122812884	34.0	31.0	34.0	31.0	34.0
3	33.00583467170936	34.0	31.0	34.0	31.0	36.0
4	35.788156339271104	37.0	35.0	37.0	33.0	37.0
5	35.844395556815584	37.0	35.0	37.0	35.0	37.0
6	35.92102706640385	37.0	35.0	37.0	35.0	37.0
7	35.75048088003544	37.0	35.0	37.0	35.0	37.0
8	35.801185096178735	37.0	36.0	37.0	34.0	37.0
9	37.021282222049884	39.0	36.0	39.0	34.0	39.0
10-11	37.14455069522603	39.0	36.0	39.0	34.0	39.0
12-13	37.01108771059331	38.0	36.5	39.0	33.5	39.0
14-15	38.10463442855869	39.0	36.5	41.0	34.5	41.0
16-17	38.12504179492707	39.0	37.0	41.0	34.5	41.0
18-19	38.13539086361532	39.0	37.0	41.0	34.0	41.0
20-21	38.02923774225847	39.0	36.5	41.0	34.0	41.0
22-23	37.944619746529774	39.0	36.5	41.0	34.0	41.0
24-25	37.81273221043539	39.0	36.0	41.0	34.0	41.0
26-27	37.68450274071199	39.0	36.5	41.0	34.0	41.0
28-29	37.64479733297058	39.0	36.5	41.0	34.5	41.0
30-31	37.48914267230725	38.5	36.0	41.0	34.0	41.0
32-33	37.340093744137135	38.0	36.5	41.0	33.0	41.0
34-35	37.16262145047331	38.0	36.0	41.0	33.0	41.0
36-37	37.17964245155012	38.0	35.5	41.0	33.0	41.0
38-39	37.3142899511828	38.0	36.0	41.0	33.0	41.0
40-41	37.211359738585784	38.0	36.5	41.0	33.0	41.0
42-43	37.238696753807766	38.0	36.5	41.0	33.0	41.0
44-45	37.07156145121068	38.0	36.0	41.0	33.0	41.0
46-47	36.804761797520584	38.0	36.0	41.0	31.5	41.0
48-49	36.76325464475356	38.0	36.5	41.0	32.0	41.0
50-51	36.36909955973891	38.0	35.0	40.0	30.5	41.0
52-53	36.42111206380035	38.0	35.5	40.0	30.5	41.0
54-55	36.23390865794741	37.5	35.5	40.0	31.0	41.0
56-57	35.93953243440716	37.0	35.0	40.0	29.0	41.0
58-59	35.81631799269567	36.5	35.0	40.0	29.5	41.0
60-61	35.55472324902185	36.5	35.0	40.0	29.0	41.0
62-63	35.375647810018464	37.5	35.0	39.5	28.5	41.0
64-65	35.244540597244736	37.0	35.0	39.0	28.0	41.0
66-67	35.02615050241997	36.5	34.5	39.0	28.0	41.0
68-69	34.561125659751426	36.0	34.0	38.5	27.0	40.0
70-71	34.31866468363624	36.0	34.0	38.0	26.5	40.0
72-73	34.05746795661719	36.0	34.0	37.0	26.0	39.5
74-75	33.77655383141202	35.5	33.5	37.5	26.0	39.0
76-77	32.29420931625951	34.5	31.0	36.5	24.5	37.5
78-79	33.30188868573866	35.0	33.0	37.0	26.0	38.0
80-81	33.29249892527331	35.0	33.0	36.5	26.0	37.0
82-83	33.10771858088492	35.0	33.0	36.0	26.0	37.5
84-85	32.900895578337355	35.0	33.0	36.5	25.0	37.0
86-87	32.65174196533958	35.0	33.0	36.0	25.0	37.0
88-89	32.4898640604673	35.0	33.0	36.0	25.0	36.5
90-91	32.34618949225134	35.0	32.5	35.5	24.5	37.0
92-93	32.19128346250335	35.0	32.0	35.5	24.0	36.5
94-95	32.068801124435645	35.0	32.0	35.0	24.0	36.0
96-97	31.895846133644604	35.0	32.0	35.0	23.0	36.5
98-99	31.76675134320405	35.0	31.5	35.0	22.0	36.5
100	30.581176781689816	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.09846281261533107
1101	2	-0.06312268989194081
1101	3	-0.10932426504613346
1101	4	-0.0923318949642109
1101	5	-0.12271511625565523
1101	6	-0.23186642925244882
1101	7	-0.14074437205798063
1101	8	-0.0926193557208137
1101	9	-0.22664019382278866
1101	10-11	-0.12613539383411876
1101	12-13	-0.18087676452233836
1101	14-15	-0.26141201813445036
1101	16-17	-0.13489319560434154
1101	18-19	-0.12224534573700652
1101	20-21	-0.19278310940494237
1101	22-23	-0.19664019895587614
1101	24-25	-0.27217774032632036
1101	26-27	-0.2979499784510722
1101	28-29	-0.3368647838057086
1101	30-31	-0.21671411920995354
1101	32-33	-0.23776977959343526
1101	34-35	-0.24009264900442417
1101	36-37	-0.38348838245784833
1101	38-39	-0.24430829764409623
1101	40-41	-0.35673393026294775
1101	42-43	-0.3991115671434926
1101	44-45	-0.4318756034107025
1101	46-47	-0.45828042158398574
1101	48-49	-0.5361941995763146
1101	50-51	-0.338544985278574
1101	52-53	-0.4546182969774506
1101	54-55	-0.5048659592064055
1101	56-57	-0.5644007832379643
1101	58-59	-0.6097004469615115
1101	60-61	-0.6048153271743075
1101	62-63	-0.34519796622531373
1101	64-65	-0.3698303086138779
1101	66-67	-0.4489520830520135
1101	68-69	-0.4034737055780653
1101	70-71	-0.43412468438190643
1101	72-73	-0.4944196383081305
1101	74-75	-0.5850521647845994
1101	76-77	-0.5713403835954125
1101	78-79	-0.37823059726167685
1101	80-81	-0.20991376236882076
1101	82-83	-0.316172850098404
1101	84-85	-0.483347293694699
1101	86-87	-0.5070798231009448
1101	88-89	-0.44476718670902926
1101	90-91	-0.5702240111765633
1101	92-93	-0.765735209342445
1101	94-95	-0.6946453748826897
1101	96-97	-0.5485753137324778
1101	98-99	-0.6086957320788038
1101	100	-0.6525021010554184
1102	1	-0.03184104422666678
1102	2	-0.07209593079151233
1102	3	-0.11342673288363869
1102	4	-0.0456938781601437
1102	5	-0.1072473723267322
1102	6	-0.15548116117687272
1102	7	-0.1519965803536678
1102	8	-0.09343219333065633
1102	9	-0.16006153361676922
1102	10-11	-0.1362148580893745
1102	12-13	-0.14052865187916552
1102	14-15	-0.21227605409271888
1102	16-17	-0.12082684971442603
1102	18-19	-0.2058859189500879
1102	20-21	-0.21571232048906808
1102	22-23	-0.13269749469795045
1102	24-25	-0.13262146004073117
1102	26-27	-0.14553288771359263
1102	28-29	-0.13937075868318516
1102	30-31	-0.13726275124941623
1102	32-33	-0.16578547138875876
1102	34-35	-0.1551857512158321
1102	36-37	-0.2144902728958087
1102	38-39	-0.26506883019584393
1102	40-41	-0.23264867651970889
1102	42-43	-0.23220637548020306
1102	44-45	-0.3318753533068204
1102	46-47	-0.26611360910444404
1102	48-49	-0.24231611382416673
1102	50-51	-0.1736201473997312
1102	52-53	-0.21776891530024045
1102	54-55	-0.29402331685612637
1102	56-57	-0.17037716134334602
1102	58-59	-0.39297800134017535
1102	60-61	-0.4125835397909583
1102	62-63	-0.2869009402175067
1102	64-65	-0.26678254273279123
1102	66-67	-0.3427754307911002
1102	68-69	-0.32546378701396605
1102	70-71	-0.31575272377799735
1102	72-73	-0.4319858246526138
1102	74-75	-0.7731050463321409
1102	76-77	-0.3410729197272566
1102	78-79	-0.2604635059441023
1102	80-81	-0.21896368442325098
1102	82-83	-0.2427438619888278
1102	84-85	-0.2897417239090814
1102	86-87	-0.3813409832397525
1102	88-89	-0.31271424861023434
1102	90-91	-0.23382559111056622
1102	92-93	-0.3325000927290773
1102	94-95	-0.487686845788069
1102	96-97	-0.45707163084863467
1102	98-99	-0.7492395232187228
1102	100	-0.4577777158784926
1103	1	-0.1707038330554127
1103	2	-0.12953240170948277
1103	3	-0.1710278955124096
1103	4	-0.060357789558459274
1103	5	-0.16728534086939106
1103	6	-0.09938961994659223
1103	7	-0.08031246992918994
1103	8	-0.07391775149874746
1103	9	-0.1129982551678097
1103	10-11	-0.11944252447272419
1103	12-13	-0.0906650881750437
1103	14-15	-0.19214351848230393
1103	16-17	-0.15783002533542856
1103	18-19	-0.15753501090068056
1103	20-21	-0.15161588516134827
1103	22-23	-0.03602014557853295
1103	24-25	-0.1599590913775515
1103	26-27	-0.2634410153459825
1103	28-29	-0.22633248952116247
1103	30-31	-0.18113714917756596
1103	32-33	-0.14890085275266784
1103	34-35	-0.06909876312379737
1103	36-37	-0.12493753278704389
1103	38-39	-0.16335594870827919
1103	40-41	-0.25076185322461697
1103	42-43	-0.20274953540726415
1103	44-45	-0.26168644717899525
1103	46-47	-0.2534299269836495
1103	48-49	-0.20312445020637426
1103	50-51	-0.10642720974509956
1103	52-53	-0.18865010540218208
1103	54-55	-0.24042084791866358
1103	56-57	-0.08348718277255784
1103	58-59	-0.16987901548331052
1103	60-61	-0.14965396014243026
1103	62-63	-0.15229067025990162
1103	64-65	-0.26377057864294784
1103	66-67	-0.33870259952135484
1103	68-69	-0.20236588857842008
1103	70-71	-0.3963140331174415
1103	72-73	-0.483858574611304
1103	74-75	-0.3210889146124458
1103	76-77	-0.28198025354209477
1103	78-79	-0.30158311492142786
1103	80-81	-0.2712235739061768
1103	82-83	-0.2728347914569298
1103	84-85	-0.2707289516196667
1103	86-87	-0.2531806299891812
1103	88-89	-0.24134214040549296
1103	90-91	-0.3083057248002419
1103	92-93	-0.3860751130480722
1103	94-95	-0.09170958552149955
1103	96-97	-0.8528985814281427
1103	98-99	-1.106961003753355
1103	100	-0.3938495853065547
1104	1	5.599599722430071E-4
1104	2	-0.05532488258608481
1104	3	-0.05320851273665994
1104	4	-0.11769515822246035
1104	5	-0.11507804430280544
1104	6	-0.13021999043500188
1104	7	-0.15867864184196634
1104	8	-0.04902483979611105
1104	9	-0.10459213293191283
1104	10-11	-0.15871538875153135
1104	12-13	-0.1314847223575697
1104	14-15	-0.2421278570764045
1104	16-17	-0.11471764375763627
1104	18-19	-0.1813927586256554
1104	20-21	-0.2560779433448488
1104	22-23	-0.18175303068425563
1104	24-25	-0.2340521036429024
1104	26-27	-0.1579556079296225
1104	28-29	-0.26245723948819233
1104	30-31	-0.28570852809566105
1104	32-33	-0.2682926612878589
1104	34-35	-0.17617878329129866
1104	36-37	-0.20760897709838133
1104	38-39	-0.15312826071645702
1104	40-41	-0.25097850267290056
1104	42-43	-0.26372168970232934
1104	44-45	-0.32617870852114805
1104	46-47	-0.209656263148851
1104	48-49	-0.24507764733127146
1104	50-51	-0.15639176551069767
1104	52-53	-0.23500326460998622
1104	54-55	-0.2576128127931554
1104	56-57	-0.17194263156800815
1104	58-59	-0.11701390552375557
1104	60-61	-0.1721481351984835
1104	62-63	-0.07359722304708072
1104	64-65	-0.2316443298518962
1104	66-67	-0.22677661065468868
1104	68-69	-0.24422370911519664
1104	70-71	-0.21843879132730848
1104	72-73	-0.21652950374482316
1104	74-75	-0.207187905688194
1104	76-77	-0.23785510104946184
1104	78-79	-0.2692062492907894
1104	80-81	-0.09599562489092506
1104	82-83	-0.13721855634899782
1104	84-85	-0.0998558007646082
1104	86-87	-0.09633494704198142
1104	88-89	-0.17971588199779376
1104	90-91	-0.14459206887858755
1104	92-93	-0.1562208328133181
1104	94-95	-0.2567432644132239
1104	96-97	0.0677070890998408
1104	98-99	-0.9823282911978559
1104	100	-0.2294252930921985
1105	1	-0.20702614756047666
1105	2	-1.0701209068928144
1105	3	-0.16176672007274817
1105	4	-0.09661004228239989
1105	5	-0.1444921147482603
1105	6	-0.12044549301145224
1105	7	-0.09846322469504543
1105	8	-0.12736648583990728
1105	9	-0.16151283518291137
1105	10-11	-0.13379606159375612
1105	12-13	-0.12982862491028868
1105	14-15	-0.19751678174926468
1105	16-17	-0.6162264854587747
1105	18-19	-0.1176527381157868
1105	20-21	-0.1978679587696206
1105	22-23	-0.14416308814578116
1105	24-25	-0.15209739484908624
1105	26-27	-0.11241809267435343
1105	28-29	-0.23612229728871625
1105	30-31	-0.17678615412115306
1105	32-33	-0.10881018578104573
1105	34-35	-0.05804656881912962
1105	36-37	-0.13918670058811244
1105	38-39	-0.21642836792710796
1105	40-41	-0.1398427900822199
1105	42-43	-0.20693791410737106
1105	44-45	-0.27535190458528547
1105	46-47	-0.24508530706708598
1105	48-49	-0.2149516642137712
1105	50-51	-0.2363844768809571
1105	52-53	-0.31735683519104185
1105	54-55	-0.3534360465717157
1105	56-57	-0.20702949127035453
1105	58-59	-0.3637676578968012
1105	60-61	-0.3056042169667421
1105	62-63	-0.26149793877357297
1105	64-65	-0.2226741772144294
1105	66-67	-0.29349432244871565
1105	68-69	-0.27447761404094706
1105	70-71	-0.42395802144460504
1105	72-73	-0.39546854198469816
1105	74-75	-0.31067161644824637
1105	76-77	-0.2417835983204597
1105	78-79	-0.3180395388358477
1105	80-81	-0.2715411597576889
1105	82-83	-0.20180344345999401
1105	84-85	-0.3065197059446163
1105	86-87	-0.31938184852683094
1105	88-89	-0.2633444330860044
1105	90-91	-0.24867619110721506
1105	92-93	-0.2739337921664955
1105	94-95	-0.30159349790362455
1105	96-97	-0.4086159878884317
1105	98-99	-0.3298979357500258
1105	100	-0.2577375939261515
1106	1	-0.13251911642078795
1106	2	-0.15781703540527303
1106	3	-0.14342003463422515
1106	4	-0.08154946982386235
1106	5	-0.11080971626994796
1106	6	-0.05748086882233849
1106	7	-0.10476757793816915
1106	8	-0.06370207013467422
1106	9	-0.09058470813233299
1106	10-11	-0.08567423349744274
1106	12-13	-0.05707600432603499
1106	14-15	-0.13471672277572821
1106	16-17	-0.06824975508407505
1106	18-19	-0.10271338325296853
1106	20-21	-0.11936048740501093
1106	22-23	-0.017500045028654654
1106	24-25	-0.0685200689248191
1106	26-27	-0.05803915245357416
1106	28-29	-0.1542277415421225
1106	30-31	-0.0918627158813905
1106	32-33	0.017644165800852818
1106	34-35	0.006661823695388591
1106	36-37	-0.09365067482249145
1106	38-39	-0.12254832506748414
1106	40-41	-0.1477715110211406
1106	42-43	-0.1662155506809313
1106	44-45	-0.1987859912846801
1106	46-47	-0.14768294563411644
1106	48-49	-0.13028415862789444
1106	50-51	-0.08650216437109037
1106	52-53	-0.1576003501794574
1106	54-55	-0.09350217375519065
1106	56-57	-0.067883371894645
1106	58-59	-0.25344692704036476
1106	60-61	-0.0891088123335777
1106	62-63	-0.08679275450205637
1106	64-65	-0.06330340814978541
1106	66-67	-0.054496052200441625
1106	68-69	-0.10645055889654742
1106	70-71	-0.11921750496939154
1106	72-73	-0.13700277777914494
1106	74-75	-0.11442967339507959
1106	76-77	-0.21534492456036247
1106	78-79	-0.11554178149114591
1106	80-81	-0.06621334792477995
1106	82-83	-0.061258260185219626
1106	84-85	0.008887180253083216
1106	86-87	-0.08982323631515499
1106	88-89	-0.13134080745992804
1106	90-91	-0.14726378548561314
1106	92-93	-0.2122106749586834
1106	94-95	-0.19033183817258248
1106	96-97	-0.27142622187213306
1106	98-99	-0.21617169128398572
1106	100	-0.14918217542175327
1107	1	-0.02609241849200572
1107	2	-0.030524095295241693
1107	3	7.359476264880982E-4
1107	4	0.005688530577749873
1107	5	0.027909433943591466
1107	6	-0.031081068391912936
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2316	86-87	-0.2494330169274761
2316	88-89	-0.12101118361507446
2316	90-91	-0.1773147096037988
2316	92-93	-0.24826249210187257
2316	94-95	-0.2228805206895359
2316	96-97	-0.13047623488788673
2316	98-99	0.006902429791406206
2316	100	-0.26710052328934
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	8.0
12	14.0
13	94.0
14	409.0
15	1133.0
16	2695.0
17	5214.0
18	8875.0
19	13360.0
20	18616.0
21	26237.0
22	33961.0
23	38856.0
24	41585.0
25	44647.0
26	49569.0
27	58454.0
28	70800.0
29	86545.0
30	108135.0
31	135719.0
32	173408.0
33	232489.0
34	354070.0
35	673728.0
36	1176136.0
37	897523.0
38	1024611.0
39	229024.0
40	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.25700827272674	19.264647319066803	13.900455494125374	23.577888914081075
2	15.924277930160672	20.831693735406066	39.90661698404739	23.337411350385878
3	18.660878703225933	26.025239429129687	30.44581739364018	24.8680644740042
4	13.096355264417275	17.569997081392817	37.93168643007484	31.40196122411507
5	13.520145137032191	37.29158300745972	34.7055168672399	14.482754988268187
6	30.285615691972396	36.944660370504906	18.50546676353069	14.264257173992009
7	26.74083476524282	32.80298348126452	22.53349996213257	17.922681791360088
8	25.450656162783076	35.16028889796581	20.720276162830295	18.66877877642082
9	26.34225509353255	16.158786033765395	20.670731164676827	36.828227708025224
10-11	24.62489667047127	26.162114961007887	28.682288453817932	20.53069991470291
12-13	25.327258435545218	23.941263839786302	28.46358348622587	22.267894238442615
14-15	22.85023587293967	25.464223719963698	26.389251790128622	25.29628861696801
16-17	22.259960895340686	28.227376221929795	27.014198255046978	22.498464627682544
18-19	22.013607960128166	27.251599243158697	28.98084504259139	21.753947754121743
20-21	22.990689765552812	26.47412953507854	28.846634262843292	21.68854643652536
22-23	22.728816100489208	26.38177700483541	28.642397321658436	22.247009573016946
24-25	22.42410065076174	26.44006756981327	28.79463146536811	22.341200314056877
26-27	22.305585305381243	26.562760386654798	28.77752782172518	22.35412648623878
28-29	22.30103096977928	26.713543845322384	28.7903864306715	22.195038754226836
30-31	22.7630874625078	26.473781845321724	28.750114204413457	22.013016487757014
32-33	22.49310423619494	26.580413160964365	28.622696280882902	22.30378632195779
34-35	22.66165317258186	26.75428626954357	28.54640304805426	22.037657509820313
36-37	22.703755934501107	26.646165380772956	28.40679798980481	22.243280694921122
38-39	22.511144677249113	26.785775097330045	28.464362505572456	22.238717719848392
40-41	22.694664096678768	26.53952945610247	28.551157970254433	22.214648476964328
42-43	22.528759994518662	26.662611058153303	28.28958570406172	22.519043243266314
44-45	22.447723025117288	26.79060331252486	28.33378437530082	22.42788928705703
46-47	22.454090347369853	26.693874674227796	28.036245977972378	22.815789000429973
48-49	22.538396743735966	26.807701928388568	27.99365989828444	22.66024142959103
50-51	22.55239205866766	27.001919532228115	27.83314464971829	22.612543759385932
52-53	22.525916522049013	27.085547930705943	27.65935361016796	22.729181937077083
54-55	22.428631635689364	26.906415708627335	27.84921558661484	22.81573706906846
56-57	22.50824114451562	27.088319641984704	27.577348703484073	22.8260905100156
58-59	22.45221528709423	27.010480548338755	27.75449604526372	22.78280811930329
60-61	22.62000201239914	26.985673098473516	27.782468734255428	22.611856154871912
62-63	22.462438704022457	26.979547453652618	27.820613901498163	22.73739994082676
64-65	22.37748787308731	26.743476122820663	27.768089566836622	23.1109464372554
66-67	22.28978607142189	26.861170638131476	27.651222696803774	23.19782059364286
68-69	22.20896304221532	26.877620220576446	27.71172254094203	23.201694196266196
70-71	22.247338143215842	26.67919233930104	27.710517115341833	23.36295240214129
72-73	22.13999041370546	26.918198830065222	27.510967116902847	23.430843639326472
74-75	22.1382173493947	26.97365589024913	27.375289998443414	23.51283676191275
76-77	22.070748006486042	26.76286753604354	27.535071814634648	23.63131264283577
78-79	21.935874953798766	26.771344063220546	27.5550714200739	23.737709562906787
80-81	21.975305973743687	26.906156307232727	27.287992360373032	23.830545358650557
82-83	22.05088616441481	26.827877564304675	27.395451778213342	23.72578449306717
84-85	22.00192608151384	26.82797258807328	27.312153701486423	23.857947628926457
86-87	21.77062308832747	26.870063205857925	27.445447011493147	23.913866694321463
88-89	21.69068990148727	26.62405427980332	27.5166550459872	24.16860077272221
90-91	21.9057636714362	26.584225498534597	27.428697161834926	24.081313668194277
92-93	21.80867868421724	26.581636262276426	27.386027011655674	24.22365804185066
94-95	21.598464290987764	26.468704725452692	27.44040082884262	24.492430154716928
96-97	21.518643636825953	26.412920685083275	27.532692202909605	24.535743475181167
98-99	21.45489017866808	26.344914465554105	27.436798155853083	24.763397199924736
100	21.417048477400666	26.392926563383728	27.49522229539782	24.694802663817782
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	961.0
1	708.0
2	903.0
3	1267.0
4	927.5
5	772.0
6	1142.5
7	2044.5
8	3482.5
9	4495.5
10	4253.0
11	3740.5
12	3821.5
13	4152.5
14	4628.5
15	5203.5
16	5662.0
17	6177.5
18	6749.5
19	7294.5
20	8198.0
21	9540.5
22	11217.5
23	13699.5
24	17115.5
25	21621.5
26	27702.5
27	34734.0
28	42619.5
29	51677.5
30	61663.0
31	71613.5
32	82117.5
33	93077.5
34	104159.0
35	114935.5
36	127955.5
37	141101.0
38	150970.5
39	158608.5
40	163550.5
41	169555.5
42	176770.5
43	184585.0
44	192302.0
45	198976.0
46	204200.5
47	208582.5
48	215312.0
49	220057.5
50	218675.5
51	212983.0
52	205586.0
53	197364.5
54	188153.5
55	175512.5
56	158790.5
57	142073.0
58	125673.0
59	107337.5
60	89321.5
61	74099.5
62	60709.0
63	47479.5
64	35551.5
65	27161.5
66	20951.5
67	16410.5
68	12811.0
69	9736.5
70	7726.0
71	6167.5
72	4973.0
73	4133.0
74	3236.0
75	2384.0
76	1685.5
77	1109.0
78	694.0
79	460.0
80	315.0
81	228.0
82	155.5
83	90.5
84	56.5
85	35.0
86	32.0
87	21.5
88	12.5
89	10.5
90	7.0
91	5.0
92	3.0
93	1.0
94	1.5
95	1.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030856960823646567
2	0.07617074378715344
3	0.002578980833995181
4	0.0
5	0.0014892706224479213
6	9.262536798151706E-4
7	0.0
8	4.3588408461890383E-4
9	0.0
10-11	2.724275528868149E-5
12-13	9.080918429560497E-5
14-15	0.011814274876858205
16-17	0.0010443056193994572
18-19	1.6345653173208894E-4
20-21	2.451847975981334E-4
22-23	0.0019342356254963859
24-25	7.627971480830817E-4
26-27	0.007564405051823894
28-29	0.008318121281477416
30-31	0.010388570683417208
32-33	0.009026432918983134
34-35	0.004422407275195962
36-37	0.003950199516858816
38-39	0.0031147550213392505
40-41	0.0034235062479443072
42-43	0.001734455420046055
44-45	0.005103476157412999
46-47	0.019142576049513527
48-49	0.019832725850160125
50-51	0.014593035916303718
52-53	0.008018450973301918
54-55	0.0010443056193994572
56-57	0.0025608189971360598
58-59	0.003350858900507823
60-61	0.0033599398189373838
62-63	0.0033417779820782622
64-65	0.003968361353717937
66-67	0.0026879518551499067
68-69	0.007836832604710709
70-71	4.7220775833714583E-4
72-73	0.0049763432993991516
74-75	0.007591647807112575
76-77	0.006965064435472901
78-79	0.004467811867343764
80-81	4.177222477597828E-4
82-83	0.0013621377644340744
84-85	9.716582719629731E-4
86-87	0.0013439759275749533
88-89	0.0011260338852655016
90-91	0.0011623575589837436
92-93	3.7231765561198033E-4
94-95	0.0010897102115472596
96-97	0.00274243736572727
98-99	0.003614205534965077
100	0.004885534115103547
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5506051.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.73677639250447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.71635250211084	50.31106133970204
2	14.038402685088712	18.176018710650446
3	4.079110200060552	7.922053347051125
4	1.6322829948830109	4.226749569961159
5	0.7994142096426604	2.58757494673138
6	0.43992877058575514	1.7087742270023658
7	0.27722298581240157	1.2562565710379916
8	0.18714373531054118	0.9692065716845237
9	0.13678080207939197	0.796927337910091
>10	0.6421728503676767	7.186623712024405
>50	0.0340418980965832	1.4814084981330407
>100	0.01608684941396862	1.8664698570736393
>500	5.810254499591736E-4	0.25696799644034324
>1k	3.75957291206697E-4	0.3897440949088797
>5k	3.417793556408868E-5	0.16589845152529423
>10k+	6.835587112817736E-5	0.6982647681630783
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16387	0.2976180206104157	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15172	0.2755513888265837	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7498	0.1361774527696892	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8161836859120993E-5
2	0.0	0.0	0.0	0.0	1.8161836859120993E-5
3	0.0	0.0	0.0	1.8161836859120993E-5	1.8161836859120993E-5
4	0.0	0.0	0.0	1.8161836859120993E-5	1.8161836859120993E-5
5	0.0	0.0	0.0	1.8161836859120993E-5	1.8161836859120993E-5
6	0.0	0.0	0.0	1.8161836859120993E-5	5.448551057736298E-5
7	0.0	0.0	0.0	1.8161836859120993E-5	5.448551057736298E-5
8	0.0	0.0	0.0	1.8161836859120993E-5	5.448551057736298E-5
9	0.0	0.0	0.0	3.6323673718241986E-5	5.448551057736298E-5
10-11	0.0	0.0	0.0	4.5404592147802486E-5	5.448551057736298E-5
12-13	0.0	0.0	0.0	5.448551057736298E-5	5.448551057736298E-5
14-15	1.8161836859120993E-5	0.0	0.0	5.448551057736298E-5	6.356642900692347E-5
16-17	3.6323673718241986E-5	0.0	0.0	5.448551057736298E-5	7.264734743648397E-5
18-19	3.6323673718241986E-5	0.0	0.0	5.448551057736298E-5	7.264734743648397E-5
20-21	3.6323673718241986E-5	0.0	0.0	7.264734743648397E-5	7.264734743648397E-5
22-23	3.6323673718241986E-5	0.0	0.0	2.905893897459359E-4	7.264734743648397E-5
24-25	3.6323673718241986E-5	0.0	0.0	8.082017402308841E-4	9.080918429560496E-5
26-27	3.6323673718241986E-5	0.0	0.0	0.0016254843988913288	9.080918429560496E-5
28-29	3.6323673718241986E-5	1.8161836859120993E-5	0.0	0.004404245438336841	9.080918429560496E-5
30-31	3.6323673718241986E-5	1.8161836859120993E-5	0.0	0.013276302744017445	9.080918429560496E-5
32-33	3.6323673718241986E-5	1.8161836859120993E-5	0.0	0.037131875458472866	9.080918429560496E-5
34-35	3.6323673718241986E-5	1.8161836859120993E-5	0.0	0.07768725716489004	9.080918429560496E-5
36-37	3.6323673718241986E-5	2.724275528868149E-5	0.0	0.13850216788765668	9.080918429560496E-5
38-39	3.6323673718241986E-5	4.5404592147802486E-5	0.0	0.2250705632766569	9.080918429560496E-5
40-41	3.6323673718241986E-5	6.356642900692347E-5	0.0	0.3217823445514762	9.080918429560496E-5
42-43	3.6323673718241986E-5	1.0897102115472596E-4	0.0	0.42149990982648	9.989010272516546E-5
44-45	3.6323673718241986E-5	1.0897102115472596E-4	0.0	0.5503308995866547	1.1805193958428644E-4
46-47	3.6323673718241986E-5	1.0897102115472596E-4	0.0	0.7051423969738021	1.5437561330252844E-4
48-49	3.6323673718241986E-5	1.0897102115472596E-4	0.0	0.8636316663249215	1.8161836859120992E-4
50-51	3.6323673718241986E-5	1.1805193958428644E-4	0.0	1.0285956305163173	1.8161836859120992E-4
52-53	3.6323673718241986E-5	1.2713285801384694E-4	0.0	1.1938411031790297	2.179420423094519E-4
54-55	3.6323673718241986E-5	1.4529469487296794E-4	0.0	1.3872646657286682	2.542657160276939E-4
56-57	3.6323673718241986E-5	1.5437561330252844E-4	0.0	1.6116359982862491	2.542657160276939E-4
58-59	3.6323673718241986E-5	1.6345653173208894E-4	0.0	1.8603078685613337	2.633466344572544E-4
60-61	4.5404592147802486E-5	1.6345653173208894E-4	0.0	2.1162081499063485	2.724275528868149E-4
62-63	5.448551057736298E-5	1.6345653173208894E-4	0.0	2.380608170901432	3.087512266050569E-4
64-65	5.448551057736298E-5	1.6345653173208894E-4	0.0	2.6534716078728655	3.087512266050569E-4
66-67	6.356642900692347E-5	1.6345653173208894E-4	0.0	2.9683978590100235	3.178321450346174E-4
68-69	7.264734743648397E-5	1.6345653173208894E-4	0.0	3.3225809205181718	3.269130634641779E-4
70-71	7.264734743648397E-5	1.9069928702077044E-4	0.0	3.6938361086738936	3.6323673718241983E-4
72-73	7.264734743648397E-5	2.1794204230945192E-4	0.0	4.058053585046705	3.6323673718241983E-4
74-75	7.264734743648397E-5	2.1794204230945192E-4	0.0	4.427528913190233	3.8139857404154083E-4
76-77	7.264734743648397E-5	2.1794204230945192E-4	0.0	4.835407445372374	3.9047949247110133E-4
78-79	7.264734743648397E-5	2.1794204230945192E-4	0.0	5.284504266306287	4.0864132933022233E-4
80-81	7.264734743648397E-5	2.1794204230945192E-4	0.0	5.751272554504126	4.1772224775978283E-4
82-83	7.264734743648397E-5	2.1794204230945192E-4	0.0	6.2300276550289855	4.1772224775978283E-4
84-85	9.080918429560496E-5	2.1794204230945192E-4	0.0	6.717191686019618	4.1772224775978283E-4
86-87	9.989010272516546E-5	2.1794204230945192E-4	0.0	7.218104227512604	4.1772224775978283E-4
88	1.0897102115472596E-4	2.1794204230945192E-4	0.0	7.6171470260627805	4.3588408461890383E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10000	0.0	50.15597	1
GTATCAA	17330	0.0	38.607037	1
TCAACGC	20490	0.0	32.131634	4
ATCAACG	20675	0.0	31.88987	3
CAACGCA	20825	0.0	31.637316	5
TATCAAC	21620	0.0	30.672365	2
AACGCAG	21565	0.0	30.638298	6
ACGCAGA	24880	0.0	26.51829	7
CGCAGAG	25655	0.0	25.721647	8
GTACATG	18745	0.0	24.573256	1
TACATGG	18815	0.0	24.221632	2
TACCTGG	4440	0.0	23.51548	2
GCAGAGT	28600	0.0	22.875834	9
ACATGGG	18985	0.0	22.871885	3
GAGTACT	16950	0.0	21.76745	12-13
GTGGTAT	5430	0.0	21.055807	1
TAACGCA	505	0.0	20.472363	4
CAGAGTA	27810	0.0	20.441488	10-11
TGGTATC	5295	0.0	20.428934	2
CATGGGG	13135	0.0	20.357237	4
>>END_MODULE