FastQCFastQC Report
Mon 6 Jun 2016
ERR522914_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522914_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8524111
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271390.31837924212859264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223800.26254937318390154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120360.14119947522973364No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138850.053.9132421
GTATCAA255500.042.5394861
TCAACGC310600.034.4065784
ATCAACG314900.033.8935783
CAACGCA315450.033.8922845
TATCAAC327350.032.6950762
AACGCAG328600.032.628036
ACGCAGA373000.028.6437237
CGCAGAG385200.027.6625298
GTACATG312500.025.3399181
GCAGAGT425500.024.5782249
TACATGG327300.023.7531492
ACATGGG328900.022.9485173
GAGTACT239400.021.95146612-13
GTGGTAT75400.021.7783621
CAGAGTA414650.021.18133710-11
TGGTATC76350.020.254362
GTACTTT257450.020.18448814-15
CATGGGG217850.020.0837424
AGAGTAC389800.020.0790310-11
Produced by FastQC (version 0.11.5)