FastQCFastQC Report
Mon 6 Jun 2016
ERR522914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8524111
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245670.28820600764114873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214770.25195589311307653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114330.1341254237538671No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134350.055.4017751
GTATCAA250650.041.596511
TCAACGC306050.033.595784
CAACGCA312450.032.937715
ATCAACG312700.032.8678323
AACGCAG326050.031.635526
TATCAAC328200.031.469922
ACGCAGA374050.027.4750657
CGCAGAG391900.026.206438
GTACATG308550.024.3672281
GCAGAGT431950.023.6242499
TACATGG321750.022.9353982
ACATGGG321400.022.2691783
GTGGTAT73650.021.911711
GAGTACT245300.021.06903612-13
TGGTATC72600.020.730762
CATGGGG212200.020.6616974
CAGAGTA418500.020.63847510-11
AGAGTAC389050.020.35219610-11
GTACTTT257100.019.95343614-15
Produced by FastQC (version 0.11.5)