FastQCFastQC Report
Mon 6 Jun 2016
ERR522913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14405862
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379830.26366350031674607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320990.22281901631433093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158950.11033702807926385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA686100.024.7047791
GTACATG536650.023.5395681
GGTATCA507600.023.3394281
TACATGG533150.023.1121332
CCGTATC68800.022.40194994
ACATGGG536600.022.0780723
TACCTGG125700.022.0752122
TCAACGC759650.021.959874
CAACGCA762900.021.852475
TATAACG10850.021.673472
ATCAACG770450.021.6582183
AACGCAG780000.021.4178356
TATCAAC785700.021.2979282
GAGTACT351250.020.93415312-13
CATGGGG320750.020.2492584
GTACTTT369650.019.57486314-15
ACGCAGA859200.019.3380727
CGTATCA49900.018.92761694
CGCAGAG881050.018.7936888
CGCCGTA100500.018.7022794
Produced by FastQC (version 0.11.5)