FastQCFastQC Report
Mon 6 Jun 2016
ERR522913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14405862
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT797800.5538023340776137No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT437490.3036888733211522RNA PCR Primer, Index 46 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358860.24910692605551824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323780.22475572791131834No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT239950.166564138959543RNA PCR Primer, Index 46 (95% over 23bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT210630.1462113131446074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157110.10905977025185998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA542500.023.8619821
TACCTGG126700.022.8528672
GTATCAA718950.022.7621671
GTACATG537550.022.4716011
TACATGG535800.021.9756452
ACATGGG536900.021.071783
GAGTACT363800.020.66292612-13
TCAACGC792000.020.3319574
CAACGCA794100.020.284395
ATCAACG805300.019.9903963
AACGCAG815250.019.7913726
TATCAAC823600.019.6440562
CATGGGG315350.019.6146264
GTACTTT376800.019.6007314-15
ACCTGGG149400.018.4044913
ACGCAGA897600.017.9126637
GTATAGG42300.017.6813181
TATAACG10650.017.6541582
AGTACTT381500.017.62234112-13
AGAGTAC660500.017.53245210-11
Produced by FastQC (version 0.11.5)