FastQCFastQC Report
Mon 6 Jun 2016
ERR522912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8877994
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT794920.8953824478818074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263420.29671117146508547No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT233180.2626494228313288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209720.23622453450633107No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC172480.19427812183698254No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC163640.1843209175406066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119660.13478269978555968No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA109980.12387933580491267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG327750.028.9754261
TACATGG335600.027.694192
ACATGGG342950.026.4852963
CATGGGG225000.024.5494694
ATGGGGG135400.021.5958735
GAGTACT238500.020.4976812-13
GTACTTT253450.018.96370914-15
GAGTACA251300.018.6145151
AGAGTAC382500.018.57642610-11
AGTACAT244300.017.511442
AGTACTT251200.017.21690612-13
CATGGGA124250.016.2689254
ACTTTTT318300.015.37960816-17
TATACCG5307.2759576E-1215.0789855
ATGGGGA93850.014.3762845
TACTTTT304600.014.02854914-15
GTATTAC19950.013.9036961
GTATAAT38600.013.7629741
GTGTAGC46450.013.4613021
CATGGGT61000.013.4093034
Produced by FastQC (version 0.11.5)