FastQCFastQC Report
Mon 6 Jun 2016
ERR522912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8877994
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT813850.9167048321952009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239140.26936265106734697No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT231920.26123018330492226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198980.22412720711458017No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC180680.2035144425643901No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC174010.1960014841190476No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG136460.15370589347098004No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA133280.1501240032376683No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA115350.12992799950078812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111520.1256139618927429No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG318550.027.682071
TACATGG330150.026.2389622
ACATGGG336950.024.9372793
CATGGGG212700.023.1770134
GAGTACT240650.020.405212-13
ATGGGGG125800.019.836425
AGAGTAC387500.019.2723110-11
GAGTACA253500.019.0717281
GTACTTT257750.018.95699314-15
AGTACAT244450.018.2477282
AGTACTT254750.018.16853112-13
CATGGGA126900.017.3684354
ACTTTTT317750.015.53713516-17
TACTTTT306000.014.95402814-15
GTATAGG21850.014.6363351
ATGGGGA93900.014.5638725
ACCGTCG7850.014.3730058
TACACTG92150.014.0244825
TGGGGGG117700.013.854876
ATACCGT11200.013.8466716
Produced by FastQC (version 0.11.5)