FastQCFastQC Report
Mon 6 Jun 2016
ERR522911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6542411
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT415170.6345825720823715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237130.3624504788830907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189090.2890218911651989No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT127600.19503513307250187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102750.15705219375548252No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC98380.15037269899430042No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA76480.11689880076320489No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG71430.10917993381950478No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG257650.026.5894051
TACATGG261050.025.8090322
ACATGGG263950.024.7612383
CATGGGG179150.023.041654
ATGGGGG111300.021.7971865
GAGTACT201050.021.11292812-13
AGAGTAC319000.020.22719810-11
GTACTTT208200.020.13037114-15
AGTACTT208450.018.84207712-13
GTATAGG19500.017.3610971
TATAGCG2454.153386E-617.2711935
GAGTACA197600.016.6567521
ACTTTTT256850.016.60914616-17
TACCTGG43750.016.0124382
CATGGGA94100.015.9880784
TACTTTT244800.015.79632714-15
GTATAGC22100.015.744131
AGTACAT194450.015.57138252
TGGGGGG122200.014.2642436
GTGTAGC34650.014.2483731
Produced by FastQC (version 0.11.5)