FastQCFastQC Report
Mon 6 Jun 2016
ERR522911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6542411
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT420960.6434325205188117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213880.32691312117199606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183850.2810126114057952No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT124400.1901439698606523No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC100440.15352138531192858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98060.14988358267311547No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC89620.13698313970186218No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA74730.11422394588172463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG257700.026.0601621
TACATGG263950.025.0357912
ACATGGG264750.024.069753
CATGGGG170600.022.3403364
ATGGGGG103250.021.5282635
GAGTACT192800.021.07909212-13
GTACTTT199800.020.16173214-15
AGAGTAC316100.019.4003810-11
AGTACTT201350.019.05164312-13
GAGTACA204150.017.5767461
AGTACAT200600.016.6585182
ACTTTTT247700.016.52589216-17
TACTTTT233550.016.24187314-15
TGGGGGG101950.015.7643776
TATACCG5103.6379788E-1215.6645045
CATGGGA94550.015.6565794
GTATAGG18500.014.9983521
GTATAGA20750.014.9585371
TATACTG29400.014.5456115
TACCTGG45000.013.9955482
Produced by FastQC (version 0.11.5)