FastQCFastQC Report
Mon 6 Jun 2016
ERR522910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7508156
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296800.39530345400388595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238070.31708185072339995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137160.18268134013198448No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92240.12285306805026427No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG117750.045.695832
ACCTGGG127150.041.3928383
GGTATCA173700.039.993221
TATAACG7550.039.2468762
GTACCTG142800.038.3051531
GTATCAA276000.034.866011
ATAACGC9950.031.6711043
TAACGCA10550.029.423894
CCTGGGG117400.029.3259144
TCAACGC331900.028.5687924
ATCAACG335650.028.2497983
CAACGCA338150.027.9971625
TATCAAC341250.027.9516072
TATCACG6100.027.7577422
AACGCAG350350.027.2221326
GTACATG283300.025.9156441
TACATGG292350.024.598912
ACGCAGA395100.024.161077
CATGGGG181300.023.9967564
ACATGGG293300.023.7174283
Produced by FastQC (version 0.11.5)