FastQCFastQC Report
Mon 6 Jun 2016
ERR522910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7508156
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277020.3689587696366458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240040.3197056640805013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137700.18340055800651983No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78110.10403353366658871No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG120700.048.407582
ACCTGGG129300.043.4048233
GGTATCA180300.040.7700651
GTACCTG146750.040.1240161
TATAACG9500.040.0784572
TAACGCA10450.037.3328484
GTATCAA279900.032.9330021
ATAACGC11850.032.525783
CCTGGGG119500.030.8767134
TCAACGC336150.027.028884
ATCAACG343100.026.4952473
CAACGCA343200.026.4877035
TATCAAC349850.025.9985222
AACGCAG356250.025.8042476
GTACATG284700.023.771221
CTGGGGG75700.023.2846935
ACGCAGA404050.022.7865967
TACATGG292700.022.5312082
CGCAGAG414050.022.2022118
ACATGGG289450.022.1824423
Produced by FastQC (version 0.11.5)