FastQCFastQC Report
Mon 6 Jun 2016
ERR522909_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522909_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10084892
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT494120.48996062625162473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328050.3252885603534475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278600.27625481760240966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153200.15191040221352892No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT123940.12289670528945675No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA103340.10247011073594046No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC100870.10002090255403825No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG142450.045.0008962
GTACCTG165250.039.800211
ACCTGGG170650.037.0965083
CCTGGGG160600.026.501164
TATAACG8300.025.4989452
CATGGGG254850.021.3690784
GTACATG489100.020.5554281
GTACCCG18650.020.1804491
TACATGG498600.020.0444642
GAGTACT278750.020.00838512-13
ACATGGG498750.019.4917963
ATAACGC10750.018.8128383
ATGGGGG144300.018.7060245
CTGGGGG101600.018.2363725
TAACGCA12650.018.2166024
GTACTTT317950.017.468114-15
TGGGGGG142800.017.146416
AGTACTT296900.016.68040512-13
TATCACG10000.015.5203572
TACCCGG21950.015.4271522
Produced by FastQC (version 0.11.5)