FastQCFastQC Report
Mon 6 Jun 2016
ERR522909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522909_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10084892
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT507090.5028214481622609No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294350.29187223819551067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267860.26560522413130455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148290.1470417333175209No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT121130.12011035913919554No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC106200.10530603599919564No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA101730.10087366329753457No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG149500.045.7077452
GTACCTG172800.040.189071
ACCTGGG174650.038.288133
TATAACG9150.029.2765832
CCTGGGG162650.028.5739354
TAACGCA12000.022.7130074
ATAACGC11750.022.396393
CATGGGG252550.020.356254
GAGTACT284350.020.00178512-13
GTACATG478650.019.6758771
TACATGG488350.018.8621642
CTGGGGG100750.018.61045
GTATAAC25850.018.3709681
GTATAGG25500.018.2543451
ACATGGG489950.018.2138213
TGGGGGG126200.017.656
GTACTTT319650.017.6146314-15
ATGGGGG140050.017.5823145
GTACCCG16850.017.5797161
AGTACTT305550.016.89874312-13
Produced by FastQC (version 0.11.5)