FastQCFastQC Report
Mon 6 Jun 2016
ERR522908_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522908_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8855610
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245380.2770898899115927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201040.22701993425636402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113160.12778340509575287No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128450.052.1929471
GTATCAA237150.041.581411
TCAACGC279650.034.70084
ATCAACG283650.034.2798583
CAACGCA286450.033.8432735
TATCAAC299200.032.754592
AACGCAG298500.032.485466
ACGCAGA341150.028.3276217
CGCAGAG352250.027.3799178
GTACATG287200.025.9478761
TACATGG295200.024.3455072
ACATGGG293000.023.979213
GCAGAGT400650.023.8254139
CATGGGG194500.021.599924
GAGTACT238350.020.87382312-13
CAGAGTA391300.020.3486510-11
GTGGTAT70150.020.1869131
AGAGTAC367150.019.72312510-11
TGGTATC69400.019.3024772
GTACTTT262200.018.8504414-15
Produced by FastQC (version 0.11.5)