FastQCFastQC Report
Mon 6 Jun 2016
ERR522908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522908_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8855610
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223980.2529244174032054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192920.21785060543542456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104310.11778974006307866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129000.053.64421
GTATCAA233350.040.0983731
TCAACGC277900.033.0747644
ATCAACG283150.032.4452823
CAACGCA285150.032.1843955
TATCAAC297350.031.1097342
AACGCAG297400.030.874166
ACGCAGA341800.026.8771887
CGCAGAG354350.025.9566358
GTACATG287700.023.8569681
GCAGAGT405400.022.4793689
TACATGG297400.022.4230752
ACATGGG294200.021.770813
GTGGTAT69350.020.5539821
GAGTACT234350.020.26603312-13
CAGAGTA396100.019.86303310-11
CATGGGG197750.019.7469944
AGAGTAC370100.019.29641210-11
TGGTATC69900.019.1073782
GTACTTT250550.018.79447614-15
Produced by FastQC (version 0.11.5)