FastQCFastQC Report
Mon 6 Jun 2016
ERR522907_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522907_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7299560
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT501580.6871373069061697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267530.3665015425587296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208070.2850445780293607No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT146710.20098471688704522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118300.16206456279556575No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC110430.15128309103562407No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC101310.1387891872934807No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA85820.11756873017003763No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA73650.10089649239132222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG58750.026.8888452
GTACATG260750.026.7057251
TACATGG272100.025.36522
ACATGGG273150.024.3216863
GTACCTG69750.023.189311
CATGGGG192300.022.5893424
GAGTACT220650.021.39996712-13
ATGGGGG116800.020.5689855
ACCTGGG75150.019.645063
AGAGTAC342500.019.49084910-11
GTACTTT241900.019.25811614-15
AGTACTT231500.018.4486212-13
TATAACG6450.018.2230512
TATCACG7000.016.7912392
GAGTACA204450.016.3744431
ATAACGC9200.016.3536573
CATGGGA89050.016.2074744
ACTTTTT292700.016.13969816-17
GTATAAC19150.015.7139311
GTATATA30350.015.4922651
Produced by FastQC (version 0.11.5)