FastQCFastQC Report
Mon 6 Jun 2016
ERR522907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7299560
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT515640.706398741841974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244880.33547227504123533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204650.2803593641260569No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT147150.20158749294478026No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC118390.16218785789828427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115330.1579958244058546No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC107400.14713215591076723No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA85380.11696595411230265No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA78170.10708864643896344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG259400.026.362631
TACATGG267400.025.2059042
TACCTGG54700.025.0046162
ACATGGG269250.024.2086093
CATGGGG185350.022.9205464
TATAACG5750.022.8878362
ATGGGGG107150.021.0520865
GAGTACT227000.020.56605512-13
GTACCTG68150.020.5657581
TAACGCA7150.019.7180654
AGAGTAC346250.019.32837310-11
GTACTTT247150.018.75243214-15
AGTACTT237450.018.38388812-13
ATAACGC8450.018.3529683
ACCTGGG76250.017.6268733
GTACACG11650.016.5520671
ACTTTTT293250.015.88948216-17
GAGTACA201700.015.6462731
TACTTTT277450.015.50164114-15
TGGGGGG100100.015.3518186
Produced by FastQC (version 0.11.5)