FastQCFastQC Report
Mon 6 Jun 2016
ERR522906_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522906_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6944441
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304030.4378034171504949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253010.364334580709952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158160.22775051296425444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121370.1747728866873518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG104100.052.126432
GTACCTG119300.046.0517961
ACCTGGG120900.044.144083
GGTATCA173150.038.222211
GTATCAA278450.034.6974371
CCTGGGG113750.033.0265924
TCAACGC338100.028.1749174
ATCAACG341300.027.8027533
TATCAAC344250.027.6601162
CAACGCA344400.027.6579235
AACGCAG355400.027.000286
GTACATG319550.026.3192121
TACATGG326450.025.235992
CATGGGG205950.024.8847374
TATAACG8200.024.6579822
ATGGGGG130000.024.0865255
ACATGGG330150.024.0843663
ACGCAGA397850.024.0129137
CGCAGAG410150.023.258258
CTGGGGG75250.023.2423345
Produced by FastQC (version 0.11.5)