FastQCFastQC Report
Mon 6 Jun 2016
ERR522906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6944441
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270680.3897793933305791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240830.34679537201050453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150670.21696490761459417No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109730.15801127837359408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG104150.051.3084642
GTACCTG119800.045.527471
ACCTGGG118700.043.9849663
GGTATCA175700.041.1314931
CCTGGGG108450.033.6691674
TATAACG7350.033.2509352
GTATCAA271300.032.9114881
TCAACGC331800.026.5703544
ATCAACG334600.026.3199183
CAACGCA336500.026.2409465
TATCAAC340900.025.9741732
TAACGCA8900.025.873014
AACGCAG348300.025.567816
GTACATG301050.025.4109731
CATGGGG198650.024.4136124
TACATGG309700.024.3568782
TATCACG5650.024.1233352
ATGGGGG125950.023.3568335
CTGGGGG72750.023.319115
ACATGGG313400.023.2719963
Produced by FastQC (version 0.11.5)