##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522906_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6944441
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.249865611933345	33.0	31.0	34.0	30.0	34.0
2	32.38163489329091	34.0	31.0	34.0	30.0	34.0
3	32.56941833043149	34.0	31.0	34.0	30.0	35.0
4	35.66750354708176	37.0	35.0	37.0	33.0	37.0
5	35.60040815380244	36.0	35.0	37.0	33.0	37.0
6	35.723513382862635	37.0	35.0	37.0	33.0	37.0
7	35.567665561562116	37.0	35.0	37.0	33.0	37.0
8	35.555030995295375	36.0	35.0	37.0	33.0	37.0
9	36.85483093599614	38.0	37.0	39.0	33.0	39.0
10-11	36.65864545468815	38.0	35.0	39.0	33.0	39.0
12-13	36.65988875130482	37.5	35.0	39.0	33.0	39.0
14-15	37.840571616923526	38.0	37.0	41.0	33.0	41.0
16-17	37.652666139722406	38.0	36.5	41.0	33.0	41.0
18-19	37.566909345187035	38.0	36.0	41.0	33.0	41.0
20-21	37.58166712050689	38.0	36.5	41.0	33.0	41.0
22-23	37.43482686367412	38.0	36.0	41.0	33.0	41.0
24-25	37.36032669872204	38.0	36.5	41.0	32.5	41.0
26-27	37.04956856858601	38.0	36.0	40.5	32.5	41.0
28-29	37.013167006530836	38.0	36.0	40.0	32.0	41.0
30-31	36.7620143219591	38.0	35.5	40.0	32.0	41.0
32-33	36.704707549534945	37.5	36.0	40.0	31.5	41.0
34-35	36.47103193763184	38.0	35.0	40.0	30.5	41.0
36-37	36.246577370302376	37.0	35.5	40.0	30.5	41.0
38-39	36.195320256878844	37.0	35.0	40.0	30.0	41.0
40-41	36.381227013088605	37.5	35.5	40.0	30.5	41.0
42-43	36.42072220067821	37.5	35.5	40.0	30.5	41.0
44-45	36.2349122845165	37.0	35.0	40.0	30.5	41.0
46-47	36.119277923161846	37.0	35.5	40.0	29.5	41.0
48-49	35.90011255045582	38.0	35.0	40.0	29.5	41.0
50-51	35.738171006132816	38.0	34.5	40.0	28.5	41.0
52-53	35.7466354743312	37.5	35.0	40.0	28.5	41.0
54-55	35.55115667337371	37.5	34.5	40.0	28.0	41.0
56-57	35.3100165297682	37.0	35.0	40.0	27.5	41.0
58-59	35.130101256530224	37.0	34.5	40.0	27.5	41.0
60-61	34.95157392798067	37.0	34.0	39.0	26.5	41.0
62-63	34.703415652894165	36.5	34.0	39.0	26.0	41.0
64-65	34.52276597929193	37.0	33.5	39.0	26.0	41.0
66-67	34.02959755868039	36.0	33.5	38.0	26.0	40.0
68-69	33.89358632321881	36.5	33.0	37.5	25.0	40.0
70-71	33.47880203748581	35.0	33.0	37.0	25.0	40.0
72-73	33.247651322835054	35.0	33.0	37.0	25.0	39.0
74-75	33.05906191729471	35.0	32.5	37.0	24.0	39.0
76-77	31.44969667104955	34.0	30.0	36.0	22.5	37.5
78-79	32.557792556665106	35.0	32.0	36.5	23.5	37.0
80-81	32.66958902811616	35.0	32.0	36.0	24.0	37.5
82-83	32.56044820598231	35.0	32.0	37.0	24.5	37.0
84-85	32.22920224968431	35.0	32.0	36.0	23.5	36.5
86-87	32.100395481796156	35.0	32.0	36.0	24.0	36.5
88-89	32.05733334043734	35.0	32.0	36.0	23.0	37.0
90-91	31.820150030794416	35.0	32.0	35.0	23.0	36.5
92-93	31.603411059291886	35.0	31.5	35.0	21.5	37.0
94-95	31.738735054412587	35.0	31.0	35.0	21.0	37.0
96-97	31.464343062314157	35.0	31.0	35.0	21.0	37.0
98-99	31.31820818983126	34.0	31.0	35.0	20.5	36.0
100	30.098338944776117	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.11406707642289149
1101	2	-0.14618232892057392
1101	3	-0.1608671041876022
1101	4	-0.14534422709259331
1101	5	-0.17361573789556672
1101	6	-0.11238204903643378
1101	7	-0.14053147144799283
1101	8	-0.25826611911500663
1101	9	-0.20012643764205507
1101	10-11	-0.20880989371180902
1101	12-13	-0.16231468349520384
1101	14-15	-0.3008854198209079
1101	16-17	-0.2862403273717007
1101	18-19	-0.4213976259047101
1101	20-21	-0.3118979428285229
1101	22-23	-0.7999913042245694
1101	24-25	-0.33358573782634693
1101	26-27	-0.32434315789740964
1101	28-29	-0.4053388741849133
1101	30-31	-0.3854267248423824
1101	32-33	-0.3261901338840332
1101	34-35	-0.486465006218296
1101	36-37	-0.5115965457974099
1101	38-39	-0.3340028683632994
1101	40-41	-0.4141335733470015
1101	42-43	-0.3157713037325607
1101	44-45	-0.4992471988774341
1101	46-47	-0.43312907824366675
1101	48-49	-0.4352258321803504
1101	50-51	-0.4191231233468855
1101	52-53	-0.5049307887066021
1101	54-55	-0.48658109960531704
1101	56-57	-0.5273586318444998
1101	58-59	-0.5284321510638392
1101	60-61	-0.6727785865660891
1101	62-63	-0.6441697357813538
1101	64-65	-0.5973431553981072
1101	66-67	-0.6076632035651599
1101	68-69	-0.6292435481196037
1101	70-71	-0.687541384296054
1101	72-73	-0.663099666488506
1101	74-75	-0.6046316438014259
1101	76-77	-0.7140980522920017
1101	78-79	-0.5894491069844747
1101	80-81	-0.5935836716492133
1101	82-83	-0.5181086771003507
1101	84-85	-0.4948190548458129
1101	86-87	-0.5582889566395615
1101	88-89	-0.5570194010843821
1101	90-91	-0.5891237960431432
1101	92-93	-0.5660321721354862
1101	94-95	-0.6436404536420746
1101	96-97	-0.7158678966514884
1101	98-99	-0.7169538563929017
1101	100	-0.7445256800046032
1102	1	-0.03845755619136071
1102	2	-0.03868889680677512
1102	3	-0.08862135771927626
1102	4	-0.027765818289971378
1102	5	-0.14370379472110528
1102	6	-0.1336735274762333
1102	7	-0.04765148039544442
1102	8	-0.17787653213203924
1102	9	-0.09855904075116939
1102	10-11	-0.23913120833802282
1102	12-13	-0.1707373859104493
1102	14-15	-0.25040994653372195
1102	16-17	-0.2643992618029003
1102	18-19	-0.2395627255069357
1102	20-21	-0.2790342464917117
1102	22-23	-0.2640210687281197
1102	24-25	-0.16526040700105682
1102	26-27	-0.26265924608638613
1102	28-29	-0.24471142522466494
1102	30-31	-0.28823965146113295
1102	32-33	-0.12309954082102337
1102	34-35	-0.2849579698556681
1102	36-37	-0.36799592385734314
1102	38-39	-0.3752874305204017
1102	40-41	-0.328550618960719
1102	42-43	-0.3484517190229681
1102	44-45	-0.2783535892394795
1102	46-47	-0.18423422978344917
1102	48-49	-0.2016119769613809
1102	50-51	-0.14272679025008728
1102	52-53	-0.22310364430499874
1102	54-55	-0.15241174453465334
1102	56-57	-0.15855840876388783
1102	58-59	-0.1968913640964729
1102	60-61	-0.3173757671946902
1102	62-63	-0.45491118497366045
1102	64-65	-0.382734329199117
1102	66-67	-0.41601839274957797
1102	68-69	-0.2739043528500673
1102	70-71	-0.24467738608981904
1102	72-73	-0.32022789002433427
1102	74-75	-0.3922827310077608
1102	76-77	-0.2926358284012345
1102	78-79	-0.39972012072523455
1102	80-81	-0.3998161911609728
1102	82-83	-0.34559553131015264
1102	84-85	-0.3443342405253418
1102	86-87	-0.3575822081837927
1102	88-89	-0.38050354683976195
1102	90-91	-0.4260702273988848
1102	92-93	-0.3220314374084552
1102	94-95	-0.4038061514622271
1102	96-97	-0.47727511040469395
1102	98-99	-0.5402915086555318
1102	100	-0.5560476486634336
1103	1	-0.09621934471451965
1103	2	-0.10742397414041704
1103	3	-0.2227086505604703
1103	4	-0.09329884628316876
1103	5	-0.19980923265886474
1103	6	-0.17215240839755097
1103	7	-0.13024581220808784
1103	8	-0.14221165260816804
1103	9	-0.14124711008504676
1103	10-11	-0.2366243627141884
1103	12-13	-0.19597222570788375
1103	14-15	-0.27717308019355613
1103	16-17	-0.3048002110098338
1103	18-19	-0.37298470956368845
1103	20-21	-0.318876706060955
1103	22-23	-0.39190450933451615
1103	24-25	-0.27873106343424325
1103	26-27	-0.3562089658225247
1103	28-29	-0.3052984877441176
1103	30-31	-0.5130641506240678
1103	32-33	-0.3390952954701234
1103	34-35	-0.45616629145047227
1103	36-37	-0.46506039482616046
1103	38-39	-0.4663727346948008
1103	40-41	-0.44157321213460676
1103	42-43	-0.4461451108271248
1103	44-45	-0.283594040085724
1103	46-47	-0.4293492716147185
1103	48-49	-0.4646857567521536
1103	50-51	-0.4480639329402649
1103	52-53	-0.3327134807206562
1103	54-55	-1.2551521493886852
1103	56-57	-0.5558978590001473
1103	58-59	-0.4899842550004436
1103	60-61	-0.5257239653283818
1103	62-63	-0.4523711077284034
1103	64-65	-0.38703798199009043
1103	66-67	-0.4451081006323534
1103	68-69	-0.43219792847874317
1103	70-71	-0.4327640797698393
1103	72-73	-0.46077726779491
1103	74-75	-0.5492592612765819
1103	76-77	-0.4702009488521135
1103	78-79	-0.4270149152374785
1103	80-81	-1.2152534020639045
1103	82-83	-0.30418243799719846
1103	84-85	-0.3289080046246582
1103	86-87	-0.2885107114099199
1103	88-89	-0.3234053536660184
1103	90-91	-0.3215588070838926
1103	92-93	-0.3535124242315959
1103	94-95	-0.3885123460528952
1103	96-97	-0.43884497220082963
1103	98-99	-0.38427819941369634
1103	100	-0.38375373070847374
1104	1	-0.07403595437419597
1104	2	-0.1514338228967418
1104	3	-0.10806439627390318
1104	4	-0.016441286343898298
1104	5	-0.060353578778816086
1104	6	-0.17017369821130757
1104	7	-0.16909163614019462
1104	8	-0.039832658586242076
1104	9	-0.12337504417589429
1104	10-11	-0.16523388132630856
1104	12-13	-0.12564269952967067
1104	14-15	-0.2981225707640718
1104	16-17	-0.26017347915975364
1104	18-19	-0.1919766761018593
1104	20-21	-0.22633012680105224
1104	22-23	-0.23864664169284566
1104	24-25	-0.11588897695698108
1104	26-27	-0.19721997193595797
1104	28-29	-0.2969941209919398
1104	30-31	-0.23148979051894258
1104	32-33	-0.1471824930130552
1104	34-35	-0.1972967379573447
1104	36-37	-0.26023824274751917
1104	38-39	-0.16504284267917058
1104	40-41	-0.18728121261052166
1104	42-43	-0.20692048309430078
1104	44-45	-0.3083809050621511
1104	46-47	-0.23186443944040747
1104	48-49	-0.28774254383763065
1104	50-51	-0.1763464776867778
1104	52-53	-0.2865207189581085
1104	54-55	-0.24174538378324684
1104	56-57	-0.3088519871921349
1104	58-59	-0.36981517007843223
1104	60-61	-0.2744689595540635
1104	62-63	-0.3691202985758437
1104	64-65	-0.48998861923112713
1104	66-67	-0.3364119102572616
1104	68-69	-0.21936782878717054
1104	70-71	-0.140086536416149
1104	72-73	-0.2840258345458153
1104	74-75	-0.29954494930383646
1104	76-77	-0.2624526318110689
1104	78-79	-0.164418415378897
1104	80-81	-0.17462919687863376
1104	82-83	-0.19036830869379884
1104	84-85	-0.7862245391220206
1104	86-87	-0.26295810812229803
1104	88-89	-0.24464522545549983
1104	90-91	-0.31745542577729324
1104	92-93	-0.29754437640699294
1104	94-95	-0.28063376845154764
1104	96-97	-0.189331968764737
1104	98-99	-0.2688430488147837
1104	100	-1.4375890034194256
1105	1	-0.04758961150490393
1105	2	-0.1685099029487631
1105	3	-0.1491268175131637
1105	4	-0.08093722787958768
1105	5	-0.12874678793656358
1105	6	-0.1541060803873009
1105	7	-0.13389323600453196
1105	8	-0.16853744744421562
1105	9	-0.19040192761308106
1105	10-11	-0.2040286941774596
1105	12-13	-0.16704430903504175
1105	14-15	-0.26428848780059866
1105	16-17	-0.24874560809795554
1105	18-19	-0.34007959788163333
1105	20-21	-0.30160620391290394
1105	22-23	-0.341175472481801
1105	24-25	-0.2269040955997994
1105	26-27	-0.2828748595632291
1105	28-29	-0.2902365095337416
1105	30-31	-0.25094112860532647
1105	32-33	-0.20069509370544125
1105	34-35	-0.29033043404876224
1105	36-37	-0.3078354120447244
1105	38-39	-0.25385751879177576
1105	40-41	-0.19521301290576787
1105	42-43	-0.19519705627485706
1105	44-45	-0.35184858435628996
1105	46-47	-0.2706212608125895
1105	48-49	-0.20624616332728607
1105	50-51	-0.11901703677775544
1105	52-53	-0.25573574064358695
1105	54-55	-1.1556967779380543
1105	56-57	-0.19783703948960607
1105	58-59	-0.19573543566493612
1105	60-61	-0.16101328368693402
1105	62-63	-0.18536530113652816
1105	64-65	-0.1212699910474484
1105	66-67	-0.20687925758536352
1105	68-69	-0.09813697666350407
1105	70-71	-0.09299193156513041
1105	72-73	-0.05365939497901451
1105	74-75	-0.13293962171044882
1105	76-77	-0.12192223657661216
1105	78-79	-0.11831133257063442
1105	80-81	-0.06355950554893042
1105	82-83	-0.039619444933897796
1105	84-85	-0.1332417849457741
1105	86-87	-0.0628642960923429
1105	88-89	-0.23549154398289573
1105	90-91	-0.2016951756848151
1105	92-93	-0.03371975156964169
1105	94-95	-0.1733412164566417
1105	96-97	-0.24943854832479317
1105	98-99	-0.0819731774507666
1105	100	-0.09809287384942067
1106	1	-0.0569489985238647
1106	2	-0.1548791818712374
1106	3	-0.15421341834444746
1106	4	-0.1051403201677843
1106	5	-0.10995619574171656
1106	6	-0.12743756329324896
1106	7	-0.10834020300469405
1106	8	-0.16104645271918372
1106	9	-0.15301371043762657
1106	10-11	-0.1423851288104956
1106	12-13	-0.16391824846217418
1106	14-15	-0.20731747976575576
1106	16-17	-0.18477257869939478
1106	18-19	-0.22504431529315383
1106	20-21	-0.23809648667978678
1106	22-23	-0.11992590708329232
1106	24-25	-0.18032448199890183
1106	26-27	-0.1202749012186004
1106	28-29	-0.11221259587153298
1106	30-31	-0.20229768687678273
1106	32-33	-0.10769311134175297
1106	34-35	-0.13726772779456553
1106	36-37	-0.2077176443737656
1106	38-39	-0.16614443958933123
1106	40-41	-0.20311819634286365
1106	42-43	-0.24342606035492764
1106	44-45	-0.17986324588159164
1106	46-47	-0.29557970917529275
1106	48-49	-0.22681146474364766
1106	50-51	-0.15083555051116804
1106	52-53	-0.2183823538221219
1106	54-55	-0.27222123967527523
1106	56-57	-0.21677682757833594
1106	58-59	-0.2744292787204827
1106	60-61	-0.19459760716488006
1106	62-63	-0.3330368121059095
1106	64-65	-0.23350146125764581
1106	66-67	-0.1789514126362448
1106	68-69	-0.17804677040308547
1106	70-71	-0.1928812793268193
1106	72-73	-0.22108461240423338
1106	74-75	-0.2432880538673885
1106	76-77	-0.27683711276808154
1106	78-79	-0.13769781631881983
1106	80-81	-0.09999035474573503
1106	82-83	-0.21476260411960624
1106	84-85	-0.16358086954017637
1106	86-87	-0.03325998882846193
1106	88-89	-0.09406489351369274
1106	90-91	-0.0950122493210408
1106	92-93	-0.05673012723959303
1106	94-95	-0.08633675551601172
1106	96-97	-0.01717840063911069
1106	98-99	-0.08986840343110103
1106	100	-0.22240325773427116
1107	1	-0.01911126154819698
1107	2	-0.08845819863990556
1107	3	-0.1326977692425899
1107	4	-0.008142633945958266
1107	5	-0.06723526724492501
1107	6	-0.11463465738689393
1107	7	-0.19821866745212446
1107	8	-0.07277954721089941
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2308	86-87	0.33039415461205124
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2308	92-93	0.4224989282787561
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2309	3	0.1107145900564177
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2311	2	0.02572874067217157
2311	3	0.025044599623250008
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2311	5	0.047019087040020224
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2311	98-99	0.23549351064049162
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2312	64-65	0.25165668482429027
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2312	82-83	0.1770181183703201
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2313	5	0.17588770687541455
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2313	14-15	0.15259572029163593
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2313	22-23	0.28998670407404603
2313	24-25	-1.0038167359019852
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2313	84-85	-0.22039793735575586
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2313	92-93	0.13695536243963957
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2314	1	-1.906116039600466
2314	2	0.06425047324316324
2314	3	-0.004832894815194777
2314	4	-0.14232907348293367
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2314	9	0.04660783088917242
2314	10-11	-0.854948085693799
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2314	18-19	0.18381831183424424
2314	20-21	-1.2512438178739629
2314	22-23	-1.3168012841956624
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2314	26-27	0.33525142874249525
2314	28-29	0.2787899108079017
2314	30-31	0.26881227250613193
2314	32-33	-2.5509478166231503
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2314	36-37	0.2845779621958755
2314	38-39	0.3269068264665691
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2314	50-51	0.2627584586154015
2314	52-53	0.2613599853182009
2314	54-55	0.2906058678399219
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2314	62-63	0.43523869700316453
2314	64-65	0.41639496182047964
2314	66-67	0.4831421855238247
2314	68-69	0.49662393556699413
2314	70-71	0.3811296003773279
2314	72-73	0.5331127489982492
2314	74-75	0.5195227604280319
2314	76-77	0.5190066421331458
2314	78-79	0.42519733356896694
2314	80-81	0.3519099378246793
2314	82-83	0.32998979986734156
2314	84-85	0.363161650024459
2314	86-87	0.3024630625987612
2314	88-89	0.2819347417068627
2314	90-91	0.2786565710380522
2314	92-93	0.09664870132311165
2314	94-95	-0.08132160812820999
2314	96-97	0.040310368045517464
2314	98-99	0.20985451356231621
2314	100	0.19923645731323347
2315	1	0.1010543909196926
2315	2	0.018615837871251983
2315	3	0.03457741669384973
2315	4	-0.0526575088614436
2315	5	0.03632288015196394
2315	6	0.02045662050357322
2315	7	0.043101481297028954
2315	8	0.03695676110553592
2315	9	0.07346112191955712
2315	10-11	0.10491253633707487
2315	12-13	0.07719909711764927
2315	14-15	0.15314954926110858
2315	16-17	0.20257686473524217
2315	18-19	0.19066420426931785
2315	20-21	0.2191837922029336
2315	22-23	0.23020375815793415
2315	24-25	0.3255416261998576
2315	26-27	0.17025368221059267
2315	28-29	-1.1409922791136893
2315	30-31	0.22645272655923065
2315	32-33	-1.4363358118911478
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2315	40-41	0.27657250961559043
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2315	44-45	-0.7039723461570233
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2315	54-55	0.3637621819065231
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2315	60-61	0.3675069211668358
2315	62-63	0.38031373187343576
2315	64-65	0.45461514379609014
2315	66-67	0.446636398245694
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2315	78-79	0.36021583189909023
2315	80-81	0.2714926585222699
2315	82-83	0.3057687014015542
2315	84-85	0.33924059342326274
2315	86-87	0.32001500978122266
2315	88-89	0.33887754155855987
2315	90-91	0.4046616850384943
2315	92-93	0.44769063793167874
2315	94-95	0.40247632457495897
2315	96-97	0.3821390274815606
2315	98-99	0.4747954767500211
2315	100	0.4342873809036263
2316	1	0.009863082129051293
2316	2	-0.1801148074528811
2316	3	-0.10596850677773517
2316	4	-0.030867073230176345
2316	5	-0.0481285193811658
2316	6	-0.03178853719439445
2316	7	0.030353782748115066
2316	8	-0.03195766376880016
2316	9	-0.06809685873876958
2316	10-11	0.057565209202131484
2316	12-13	0.035473613040416296
2316	14-15	0.07889413637665399
2316	16-17	-1.2549026600751532
2316	18-19	0.011568936891031001
2316	20-21	-0.03787934511316138
2316	22-23	-0.01877623015506913
2316	24-25	0.13778146050150042
2316	26-27	-0.02533141717476184
2316	28-29	0.00801294944051989
2316	30-31	0.025792197095462654
2316	32-33	0.09154868876510136
2316	34-35	0.08012522143229717
2316	36-37	0.02888783012098628
2316	38-39	0.1515692000966311
2316	40-41	0.012833863899849973
2316	42-43	0.05954109935964169
2316	44-45	0.04267146661852195
2316	46-47	0.08611704466679271
2316	48-49	0.04229904796548567
2316	50-51	0.05421489692682968
2316	52-53	0.028767516684155225
2316	54-55	0.1068393191430701
2316	56-57	0.03453445245266806
2316	58-59	0.025340962572251158
2316	60-61	-1.263731472146489
2316	62-63	0.13398152134841013
2316	64-65	0.11406692575711475
2316	66-67	-0.11139749404426169
2316	68-69	0.13648341298522837
2316	70-71	0.0843289383678254
2316	72-73	0.18904791174212932
2316	74-75	0.018514925928670323
2316	76-77	-0.2264116443495361
2316	78-79	-0.2855101539235463
2316	80-81	-0.12077113106284543
2316	82-83	-0.10570077014427426
2316	84-85	-0.6702781899053214
2316	86-87	-0.10926015167983394
2316	88-89	0.011307396633682743
2316	90-91	0.055243281384317555
2316	92-93	0.17455680958367736
2316	94-95	0.14640535819496137
2316	96-97	0.1608414649240082
2316	98-99	0.20340368395863706
2316	100	-0.0062499761830672185
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	1.0
10	2.0
11	8.0
12	44.0
13	226.0
14	940.0
15	2527.0
16	5490.0
17	10603.0
18	17367.0
19	24579.0
20	32802.0
21	42901.0
22	54027.0
23	61887.0
24	67270.0
25	73981.0
26	84387.0
27	98877.0
28	119107.0
29	145205.0
30	177452.0
31	218885.0
32	273597.0
33	354319.0
34	507136.0
35	894547.0
36	1413347.0
37	1026950.0
38	1016390.0
39	219414.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.26548434155406	18.12044191540472	13.007492323415507	23.606581419625723
2	16.67746299604909	20.46554886013516	39.2426218921909	23.614366251624848
3	19.04913584837138	26.292742641200352	29.769667565755114	24.888453944673156
4	13.272827209064658	16.548636668197393	36.512360065727584	33.66617605701037
5	13.562747293906085	36.642662258714516	33.64030885809166	16.154281589287745
6	30.66715242697755	35.217324939087966	19.03326978129266	15.082252852641826
7	27.71989213239543	32.30510052719882	21.97427322783021	18.000734112575543
8	26.912412600881137	33.981929415424005	20.605549623547486	18.50010836014737
9	27.64514811199346	15.89359028322078	20.292734865196493	36.16852673958926
10-11	25.466497332146098	25.481775743024325	28.17314485927914	20.87858206555043
12-13	25.712673113859218	23.52743599247626	28.19122495218462	22.568665941479903
14-15	23.441924830975438	24.816631277920077	26.27894368497916	25.462500206125316
16-17	22.765420226581746	27.267131477058214	27.021413275306195	22.946035021053845
18-19	22.47002727002727	26.312008712008712	29.02966582966583	22.188298188298187
20-21	23.332363244051518	25.818079027380136	28.911624626424427	21.937933102143923
22-23	23.161982401894825	25.47414572696441	28.79716905055942	22.566702820581348
24-25	22.704818125053283	25.624601100903906	28.849419479409317	22.821161294633498
26-27	22.568525065349153	25.629527713881068	28.896261644621728	22.905685576148045
28-29	22.59992286317604	26.018419225100402	28.867664260677532	22.51399365104603
30-31	23.462640392317933	25.6012092014754	28.925893997427327	22.01025640877934
32-33	23.073888324006106	25.770046120212957	28.803520663440967	22.352544892339964
34-35	23.236431998552945	25.95436711270717	28.636161885686207	22.173039003053674
36-37	23.135723028516594	25.95809134961661	28.49681516463053	22.409370457236268
38-39	23.028731966647594	26.172509020906382	28.31969138902157	22.479067623424445
40-41	23.09722219022032	25.85015449237561	28.468762816761213	22.583860500642857
42-43	22.888863639047468	26.119314429362706	28.15696223094158	22.83485970064825
44-45	22.589974265293574	26.209747218073915	28.342271595479794	22.858006921152718
46-47	22.7114099044033	25.958977068083698	28.01183033631096	23.317782691202037
48-49	22.81331979781408	26.15532418814429	27.985903069234443	23.045452944807185
50-51	22.721081959164263	26.396353065740836	27.925311415471977	22.957253559622924
52-53	22.88378468602806	26.522903097306518	27.48175814262421	23.111554074041216
54-55	22.652234602076824	26.300274748576758	27.862125147623896	23.185365501722526
56-57	22.840723290410896	26.52599582324931	27.369302293699132	23.263978592640658
58-59	22.706988202428178	26.484273480615638	27.71481758257141	23.093920734384767
60-61	23.181346798830376	26.43959588820504	27.707814785046054	22.671242527918537
62-63	22.96594536916637	26.538203999464322	27.71072643831099	22.78512419305832
64-65	22.842310183517178	26.2242278618597	27.604609918354566	23.328852036268554
66-67	22.70642883860277	26.35359062319886	27.51296823435091	23.42701230384747
68-69	22.457292074217104	26.39724429784589	27.652983243998037	23.49248038393897
70-71	22.545091010022063	26.198019923168616	27.724632448103176	23.53225661870615
72-73	22.420725051944178	26.7091969185656	27.18182647516101	23.688251554329216
74-75	22.573780160406194	26.643755953380865	27.14741674483301	23.635047141379932
76-77	22.424972456700985	26.308359806174092	27.41249669772028	23.854171039404648
78-79	22.274876056681812	26.451273562370915	27.414753530012987	23.859096850934286
80-81	22.356569556569557	26.640660240660242	27.006600606600607	23.996169596169597
82-83	22.512084953888607	26.61574202675286	27.10921307722404	23.762959942134497
84-85	22.490433855995736	26.82477459396313	26.750311851434518	23.934479698606616
86-87	22.231824654640032	26.811796116623686	27.003014362176486	23.953364866559788
88-89	22.021933704676695	26.4828276316266	27.23972958746125	24.255509076235455
90-91	22.62094654247855	26.405399968406247	27.11751939797342	23.856134091141783
92-93	22.400159207281373	26.490098649186123	26.99827746246403	24.111464681068476
94-95	22.11331572115699	26.342830046146283	27.032389086057073	24.511465146639654
96-97	21.997272591124833	26.285165430164508	27.2018930579236	24.51566892078706
98-99	21.965487515182165	26.240930750893398	27.039536594481795	24.754045139442642
100	21.787040579710563	26.460224026993718	27.15071976379346	24.602015629502258
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2976.0
1	2299.0
2	4751.0
3	6581.0
4	3816.5
5	2612.0
6	3601.5
7	6375.5
8	9764.5
9	11426.0
10	9999.5
11	7962.5
12	7921.0
13	8537.5
14	9107.5
15	9699.0
16	10429.5
17	11113.5
18	11787.0
19	12382.5
20	13300.0
21	14678.0
22	16470.5
23	18988.5
24	22416.5
25	26829.0
26	32565.0
27	39500.5
28	46765.5
29	54989.5
30	65228.5
31	75800.0
32	87168.0
33	99584.5
34	112409.0
35	124882.5
36	141186.0
37	157544.0
38	167732.5
39	176576.0
40	182308.0
41	186730.5
42	192797.0
43	201575.5
44	211869.0
45	222455.5
46	233401.5
47	243762.5
48	257973.0
49	274795.5
50	288079.0
51	286718.0
52	276649.5
53	270292.5
54	262641.5
55	248529.5
56	228018.0
57	209135.5
58	191662.0
59	167713.0
60	138646.0
61	112128.5
62	91648.0
63	72845.5
64	55533.0
65	42852.0
66	32649.5
67	25317.0
68	19914.0
69	15211.0
70	11959.0
71	9492.5
72	7897.0
73	6715.0
74	5380.0
75	4053.5
76	2923.5
77	1939.0
78	1282.0
79	861.0
80	584.5
81	415.5
82	271.5
83	166.5
84	129.0
85	90.0
86	52.0
87	33.5
88	20.5
89	17.5
90	14.0
91	6.0
92	6.5
93	4.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04612322287711855
2	0.010411205163957762
3	0.0
4	2.8800014284807084E-4
5	3.1680015713287795E-4
6	1.4400007142403542E-5
7	2.8800014284807084E-5
8	8.640004285442126E-5
9	0.0
10-11	2.1600010713605316E-5
12-13	3.312001642752815E-4
14-15	0.01223280606747181
16-17	0.0014976007428099685
18-19	5.0400024998412396E-5
20-21	9.072004499714232E-4
22-23	8.20800407117002E-4
24-25	0.004737602349850765
26-27	0.01671120828875931
28-29	0.013464006678147311
30-31	0.009864004892546427
32-33	0.007524003731905851
34-35	0.009748804835407198
36-37	0.005205602581978881
38-39	0.004233602099866641
40-41	0.005889602921243049
42-43	0.007243203592628982
44-45	0.007358403649768211
46-47	0.015912007892355917
48-49	0.017676008767300348
50-51	0.017056808460176997
52-53	0.011448005678210816
54-55	0.00394560195701857
56-57	0.004132802049869816
58-59	0.010425605171100165
60-61	0.002512801246349418
62-63	1.5840007856643897E-4
64-65	3.312001642752815E-4
66-67	9.864004892546426E-4
68-69	5.760002856961417E-5
70-71	1.944000964224478E-4
72-73	0.0029880014820487352
74-75	0.0055080027319693555
76-77	0.005392802674830127
78-79	0.003434401703463245
80-81	5.0400024998412396E-5
82-83	8.928004428290197E-4
84-85	4.10400203558501E-4
86-87	2.8800014284807084E-4
88-89	2.0880010356485136E-4
90-91	4.320002142721063E-4
92-93	5.04000249984124E-4
94-95	4.320002142721063E-5
96-97	0.0017424008642308285
98-99	3.816001892736939E-4
100	1.4400007142403542E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6944441.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.8795291444513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.77444865879063	38.671734983382336
2	16.30780632256727	17.57313852077661
3	5.468570630662255	8.839320320197722
4	2.3812231795146497	5.131967348004103
5	1.205178413334956	3.2467222722772173
6	0.6955531683974778	2.2485646324912407
7	0.44228967572538674	1.6681251631475122
8	0.2976840355290711	1.2831260534501172
9	0.22323441927478319	1.082498885942431
>10	1.0962582084584682	10.440568262377143
>50	0.06632779443639646	2.4557813733003635
>100	0.037302301770722086	3.662266746922413
>500	0.002808038423226762	1.0013286273828028
>1k	0.0011018850419828175	1.0078374550309002
>5k	7.108935754381453E-5	0.20871092797338753
>10k+	1.4217871508762905E-4	1.4783084273438938
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27068	0.3897793933305791	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24083	0.34679537201050453	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15067	0.21696490761459417	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10973	0.15801127837359408	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.4400007142403542E-5	0.0	1.4400007142403542E-5	0.0
5	0.0	1.4400007142403542E-5	0.0	1.4400007142403542E-5	0.0
6	0.0	1.4400007142403542E-5	0.0	1.4400007142403542E-5	2.8800014284807084E-5
7	0.0	1.4400007142403542E-5	0.0	1.4400007142403542E-5	2.8800014284807084E-5
8	0.0	1.4400007142403542E-5	0.0	1.4400007142403542E-5	5.760002856961417E-5
9	0.0	1.4400007142403542E-5	0.0	1.4400007142403542E-5	8.640004285442125E-5
10-11	7.200003571201771E-6	1.4400007142403542E-5	0.0	2.1600010713605313E-5	8.640004285442125E-5
12-13	1.4400007142403542E-5	1.4400007142403542E-5	0.0	2.8800014284807084E-5	8.640004285442125E-5
14-15	1.4400007142403542E-5	1.4400007142403542E-5	0.0	4.3200021427210625E-5	1.1520005713922833E-4
16-17	4.3200021427210625E-5	1.4400007142403542E-5	0.0	5.0400024998412396E-5	1.5840007856643897E-4
18-19	4.3200021427210625E-5	1.4400007142403542E-5	0.0	6.480003214081594E-5	1.9440009642244783E-4
20-21	4.3200021427210625E-5	1.4400007142403542E-5	0.0	1.0800005356802656E-4	2.0160009999364959E-4
22-23	4.3200021427210625E-5	1.4400007142403542E-5	0.0	2.952001464192726E-4	2.3760011784965845E-4
24-25	4.3200021427210625E-5	1.4400007142403542E-5	0.0	8.856004392578179E-4	2.592001285632638E-4
26-27	4.3200021427210625E-5	1.4400007142403542E-5	0.0	0.0017424008642308287	2.592001285632638E-4
28-29	4.3200021427210625E-5	1.4400007142403542E-5	0.0	0.004831202396276388	2.592001285632638E-4
30-31	4.3200021427210625E-5	1.4400007142403542E-5	0.0	0.013507206699574522	2.592001285632638E-4
32-33	4.3200021427210625E-5	1.4400007142403542E-5	0.0	0.0341352169310676	2.808001392768691E-4
34-35	4.3200021427210625E-5	1.4400007142403542E-5	0.0	0.06858003401569687	2.8800014284807084E-4
36-37	4.3200021427210625E-5	2.1600010713605313E-5	0.0	0.1214784602533163	2.952001464192726E-4
38-39	4.3200021427210625E-5	2.8800014284807084E-5	1.4400007142403542E-5	0.20266570052218746	3.240001607040797E-4
40-41	4.3200021427210625E-5	2.8800014284807084E-5	1.4400007142403542E-5	0.2971153473692123	3.312001642752815E-4
42-43	4.3200021427210625E-5	2.8800014284807084E-5	1.4400007142403542E-5	0.3890953929913149	3.312001642752815E-4
44-45	4.3200021427210625E-5	2.8800014284807084E-5	1.4400007142403542E-5	0.4992842476449868	3.528001749888868E-4
46-47	4.3200021427210625E-5	2.8800014284807084E-5	1.4400007142403542E-5	0.6261339105624196	3.744001857024921E-4
48-49	4.3200021427210625E-5	2.8800014284807084E-5	1.4400007142403542E-5	0.7609179774153167	4.0320019998729917E-4
50-51	4.3200021427210625E-5	2.8800014284807084E-5	1.4400007142403542E-5	0.9067684497571511	4.0320019998729917E-4
52-53	4.3200021427210625E-5	2.8800014284807084E-5	1.4400007142403542E-5	1.0585229250273709	4.1040020355850095E-4
54-55	4.3200021427210625E-5	3.6000017856008854E-5	1.4400007142403542E-5	1.2347286124253918	4.248002107009045E-4
56-57	5.0400024998412396E-5	4.3200021427210625E-5	1.4400007142403542E-5	1.434672711597665	4.4640022141450984E-4
58-59	5.760002856961417E-5	4.3200021427210625E-5	1.4400007142403542E-5	1.65991042331557	4.5360022498571156E-4
60-61	5.760002856961417E-5	4.3200021427210625E-5	1.4400007142403542E-5	1.890893737883294	4.6080022855691334E-4
62-63	5.760002856961417E-5	4.3200021427210625E-5	1.4400007142403542E-5	2.134858658889895	4.6080022855691334E-4
64-65	5.760002856961417E-5	4.3200021427210625E-5	1.4400007142403542E-5	2.3732867771502413	4.6080022855691334E-4
66-67	5.760002856961417E-5	4.3200021427210625E-5	1.4400007142403542E-5	2.6463109125702124	4.6080022855691334E-4
68-69	5.760002856961417E-5	5.760002856961417E-5	1.4400007142403542E-5	2.9541470652569446	4.6080022855691334E-4
70-71	5.760002856961417E-5	5.760002856961417E-5	1.4400007142403542E-5	3.2893720315285275	4.752002356993169E-4
72-73	5.760002856961417E-5	7.200003571201771E-5	1.4400007142403542E-5	3.6045593878614564	6.048002999809488E-4
74-75	5.760002856961417E-5	7.200003571201771E-5	1.4400007142403542E-5	3.9318931502190027	6.192003071233523E-4
76-77	5.760002856961417E-5	7.200003571201771E-5	1.4400007142403542E-5	4.292354129007649	6.192003071233523E-4
78-79	5.760002856961417E-5	7.200003571201771E-5	1.4400007142403542E-5	4.703582332976837	6.192003071233523E-4
80-81	6.480003214081594E-5	7.200003571201771E-5	1.4400007142403542E-5	5.118460938756625	6.624003285505629E-4
82-83	7.200003571201771E-5	7.200003571201771E-5	1.4400007142403542E-5	5.548315551964514	7.488003714049842E-4
84-85	7.200003571201771E-5	8.640004285442125E-5	1.4400007142403542E-5	5.9824037672722685	7.488003714049842E-4
86-87	7.200003571201771E-5	8.640004285442125E-5	1.4400007142403542E-5	6.431223189886702	7.632003785473878E-4
88	7.200003571201771E-5	8.640004285442125E-5	1.4400007142403542E-5	6.787716966707616	7.632003785473878E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	10415	0.0	51.308464	2
GTACCTG	11980	0.0	45.52747	1
ACCTGGG	11870	0.0	43.984966	3
GGTATCA	17570	0.0	41.131493	1
CCTGGGG	10845	0.0	33.669167	4
TATAACG	735	0.0	33.250935	2
GTATCAA	27130	0.0	32.911488	1
TCAACGC	33180	0.0	26.570354	4
ATCAACG	33460	0.0	26.319918	3
CAACGCA	33650	0.0	26.240946	5
TATCAAC	34090	0.0	25.974173	2
TAACGCA	890	0.0	25.87301	4
AACGCAG	34830	0.0	25.56781	6
GTACATG	30105	0.0	25.410973	1
CATGGGG	19865	0.0	24.413612	4
TACATGG	30970	0.0	24.356878	2
TATCACG	565	0.0	24.123335	2
ATGGGGG	12595	0.0	23.356833	5
CTGGGGG	7275	0.0	23.31911	5
ACATGGG	31340	0.0	23.271996	3
>>END_MODULE